{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T17:56:00Z","timestamp":1779299760376,"version":"3.51.4"},"reference-count":32,"publisher":"Microbiology Society","issue":"4","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS","award":["22310130"],"award-info":[{"award-number":["22310130"]}]},{"name":"JSPS","award":["18H02128"],"award-info":[{"award-number":["18H02128"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,20]]},"abstract":"\n Geobacillus kaustophilus<\/jats:italic>\n HTA426 is a thermophilic Gram-positive bacterium that encodes the\n myo<\/jats:italic>\n -inositol-inducible\n iol<\/jats:italic>\n operons, the products of which form the\n myo<\/jats:italic>\n -inositol catabolic pathway. Strain PS8 is a mutant of HTA426 that is defective in\n myo<\/jats:italic>\n -inositol catabolism due to constitutive repression of the\n iol<\/jats:italic>\n operons. Several spontaneous suppressor mutants of PS8, which restored growth on\n myo<\/jats:italic>\n -inositol, were obtained. These suppressors had mutations that may affect the translation of\n crh<\/jats:italic>\n . Inclusion of a plasmid-based copy of\n crh<\/jats:italic>\n into the suppressor mutants concomitantly restored the repression of the\n iol<\/jats:italic>\n operons. PS8 and its suppressor mutants shared a mutated allele of\n hprK<\/jats:italic>\n (G268R) that may encode a defective HPr kinase\/phosphorylase, which could lead to the accumulation of the phosphorylated form of Crh. Inclusion of a plasmid-based copy of the wild-type\n hprK<\/jats:italic>\n gene into PS8 restored the induction of the\n iol<\/jats:italic>\n operons. Conversely, inclusion of a plasmid-based copy of\n hprK<\/jats:italic>\n (G268R) into HTA426 significantly repressed the\n iol<\/jats:italic>\n operons. In HTA426, ribose, rather than glucose, repressed the\n iol<\/jats:italic>\n operons. During the growth on ribose, the expression of\n ptsH<\/jats:italic>\n , which encodes HPr, was kept at a low level, while that of\n crh<\/jats:italic>\n was elevated. Deletion of\n hprK<\/jats:italic>\n ,\n ccpA<\/jats:italic>\n (encoding global regulator CcpA) or the\n rbs<\/jats:italic>\n operon (encoding the components for ribose catabolism) abolishes the ribose-induced repression of\n iol<\/jats:italic>\n operons. These results suggest an atypical catabolite repression of the\n iol<\/jats:italic>\n operons of\n G. kaustophilus<\/jats:italic>\n HTA426, where HPrK phosphorylates Crh in the presence of ribose, and the phosphorylated form of Crh cooperates with CcpA to repress the transcription of the\n iol<\/jats:italic>\n operons.\n <\/jats:p>","DOI":"10.1099\/mic.0.001702","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T17:16:46Z","timestamp":1777396606000},"source":"Crossref","is-referenced-by-count":1,"title":["Atypical catabolite repression of Geobacillus kaustophilus iol operons in the presence of ribose"],"prefix":"10.1099","volume":"172","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3383-4664","authenticated-orcid":false,"given":"Ken-ichi","family":"Yoshida","sequence":"first","affiliation":[{"name":"Department of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaho","family":"Fukui","sequence":"additional","affiliation":[{"name":"Department of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuma","family":"Osawa","sequence":"additional","affiliation":[{"name":"Department of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moeka","family":"Tsuji","sequence":"additional","affiliation":[{"name":"Department of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miyuki","family":"Kado-Matsushita","sequence":"additional","affiliation":[{"name":"Department of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, 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