{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:10:03Z","timestamp":1792786203081,"version":"4.5.0"},"reference-count":62,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Biotechnology and Biological Sciences Research Council","award":["BB\/X01651X"],"award-info":[{"award-number":["BB\/X01651X"]}],"id":[{"id":"https:\/\/ror.org\/00cwqg982","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Biotechnology and Biological Sciences Research Council","award":["n.a."],"award-info":[{"award-number":["n.a."]}],"id":[{"id":"https:\/\/ror.org\/00cwqg982","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,15]]},"abstract":"<jats:p>\n                    Inorganic phosphate (Pi) limitation is a pervasive environmental constraint that shapes microbial behaviour in soil and plant-associated niches. To determine how Pi scarcity influences the pathogenic potential of rice-infecting\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    species, we performed comparative proteomic and lipidomic analyses of\n                    <jats:italic>Burkholderia glumae<\/jats:italic>\n                    and\n                    <jats:italic>Burkholderia plantarii<\/jats:italic>\n                    grown under Pi-replete and Pi-deplete conditions. Pi starvation triggered strong activation of the Pho regulon in both organisms, including induction of alkaline phosphatases, phosphonate transporters and the high-affinity PstSABC uptake system. Beyond classical phosphate-stress responses, Pi limitation markedly enriched proteins associated with virulence, secretion systems, toxins, chemotaxis and cell envelope modification. Both species also activated PlcP\/Agt-mediated lipid remodelling pathways and synthesized phosphorus-free glycolipids exclusively during Pi depletion, indicating substantial membrane lipid restructuring. These results demonstrate that Pi limitation acts as a major environmental cue coordinating nutrient acquisition with multiple virulence-related functions. Our findings provide new insight into how phosphate scarcity shapes the environmental fitness and pathogenic capacity of\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    phytopathogens and highlight nutrient limitation as an important driver of plant disease emergence.\n                  <\/jats:p>","DOI":"10.1099\/mic.0.001758","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:16:15Z","timestamp":1792080975000},"source":"Crossref","is-referenced-by-count":0,"title":["Proteomics insight into phosphate limitation response in Burkholderia phytopathogens"],"prefix":"10.1099","volume":"172","author":[{"given":"Lixian","family":"Xu","sequence":"first","affiliation":[{"name":"School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manpreet","family":"Kaur","sequence":"additional","affiliation":[{"name":"School of Biosciences, University 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