{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:55:58Z","timestamp":1792083358257,"version":"4.4.0"},"reference-count":45,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,15]]},"abstract":"<jats:p>\n                    Carbapenem resistance in\n                    <jats:italic>Enterobacteriaceae<\/jats:italic>\n                    poses a major threat to public health because of the limited therapeutic options. The dissemination of the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC<\/jats:sub>\n                    gene, which encodes KPC carbapenemases, typically occurs through Tn\n                    <jats:italic>4401<\/jats:italic>\n                    transposons and plasmids that facilitate its transmission. In this study, we analysed 802 genomes of ESKAPE pathogens and other\n                    <jats:italic>Enterobacteriaceae<\/jats:italic>\n                    from Ecuador, including both clinical and non-clinical isolates, to determine the prevalence and genetic context of\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC-2<\/jats:sub>\n                    and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC-3<\/jats:sub>\n                    and their associated mobile genetic elements. Whole-genome analysis revealed several isolates carrying\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC<\/jats:sub>\n                    , with\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC-3<\/jats:sub>\n                    restricted to\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    . While most isolates harboured the Tn\n                    <jats:italic>4401a<\/jats:italic>\n                    transposon, we identified a novel variant, Tn\n                    <jats:italic>4401l<\/jats:italic>\n                    , in two\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolates collected between 2020 and 2021. Additionally, two putative plasmids carrying Tn\n                    <jats:italic>4401a<\/jats:italic>\n                    were reconstructed: pECU0072020, a mosaic multidrug-resistant plasmid with a unique genetic organization, and pECU0062021, a more compact structure carrying only\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC-2<\/jats:sub>\n                    . The findings demonstrate the wide distribution of\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC<\/jats:sub>\n                    within diverse bacterial hosts across multiple regions of Ecuador, the emergence of a novel Tn\n                    <jats:italic>4401l<\/jats:italic>\n                    variant and the presence of putative plasmids that could potentially accelerate multidrug resistance dissemination. This study highlights the need for continuous genomic surveillance and coordinated strategies and practices regarding the use of antibiotics to mitigate the risk of outbreaks and interspecies transmission of carbapenem resistance.\n                  <\/jats:p>","DOI":"10.1099\/mic.0.001772","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:16:03Z","timestamp":1792080963000},"source":"Crossref","is-referenced-by-count":0,"title":["Genomic characterization of bla\n            KPC transmission via Tn4401a and Tn4401l transposons across Enterobacteriaceae and ESKAPE in Ecuador"],"prefix":"10.1099","volume":"172","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6839-4636","authenticated-orcid":false,"given":"Isa\u00edas","family":"Mejia-Limones","sequence":"first","affiliation":[{"name":"Omics Science Laboratory, Faculty of Health Science, Universidad Esp\u00edritu Santo, Samborond\u00f3n, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8181-7074","authenticated-orcid":false,"given":"Mar\u00eda","family":"Benites-Villegas","sequence":"additional","affiliation":[{"name":"Omics Science Laboratory, Faculty of Health Science, Universidad Esp\u00edritu Santo, Samborond\u00f3n, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8554-1735","authenticated-orcid":false,"given":"Mar\u00eda","family":"C\u00e1ceres-Valdiviezo","sequence":"additional","affiliation":[{"name":"Omics Science Laboratory, Faculty of Health Science, Universidad Esp\u00edritu Santo, Samborond\u00f3n, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2651-5884","authenticated-orcid":false,"given":"Derly","family":"Andrade-Molina","sequence":"additional","affiliation":[{"name":"Omics Science Laboratory, Faculty of Health Science, Universidad 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