{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:58:04Z","timestamp":1791219484020,"version":"4.1.0"},"reference-count":37,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Aeronautics and Space Administration","award":["80NSSC18K1463"],"award-info":[{"award-number":["80NSSC18K1463"]}],"id":[{"id":"https:\/\/ror.org\/027ka1x80","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,5]]},"abstract":"<jats:p>\n                    <jats:italic>Streptococcus mutans<\/jats:italic>\n                    is a cariogenic member of human dental plaque biofilm. Our previous study of\n                    <jats:italic>S. mutans<\/jats:italic>\n                    early stationary-phase rotary cell culture system (RCCS) cultures revealed significant transcriptome changes under low-shear modelled microgravity (LSMMG) compared to normal gravity (NG), as well as an altered cell clumping phenotype. In this current study,\n                    <jats:italic>S. mutans<\/jats:italic>\n                    mid-exponential phase and late-exponential phase cultures were compared using the RCCS (LSMMG and NG orientations) as well as simulated microgravity (\u20180\u2009g\u2019) using a random positioning machine (0\u2009g RPM). The previously described stationary-phase LSMMG-altered cell clumping phenotype was also observed at earlier growth phases in LSMMG and 0\u2009g RPM. Significant fold-change differences in gene expression (late-exponential\/mid-exponential phase) were observed in LSMMG, NG and 0\u2009g RPM, with 24 genes shared between LSMMG and 0\u2009g RPM cultures (including upregulated pyrimidine biosynthesis genes and decreased genes involved in purine biosynthesis, iron transport and malolactic fermentation). Additional significant functional enrichments included TCA cycle (LSMMG and NG), starch and sucrose metabolism (LSMMG and 0\u2009g RPM) and folate biosynthesis (LSMMG, 0\u2009g RPM and NG). Overall, these data provide insight into\n                    <jats:italic>S. mutans<\/jats:italic>\n                    physiology in response to simulated microgravity. Further investigation of transcriptome changes shared between LSMMG and 0\u2009g RPM may allow us to better predict how this bacterium responds to spaceflight conditions.\n                  <\/jats:p>","DOI":"10.1099\/mic.0.001776","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:16:55Z","timestamp":1791217015000},"source":"Crossref","is-referenced-by-count":0,"title":["Comparison of Streptococcus mutans growth-phase-dependent transcriptome changes in two different simulated microgravity models"],"prefix":"10.1099","volume":"172","author":[{"given":"Ke Aira T.","family":"Davis","sequence":"first","affiliation":[{"name":"Present address: Health Sciences (Biotechnology), Central Georgia Technical College, 12 Macon, GA 31206, USA"},{"name":"Department of Microbiology and Cell Science, IFAS, University of Florida, Gainesville, Florida, 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1335-4409","authenticated-orcid":false,"given":"Kelly C.","family":"Rice","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science, IFAS, University of Florida, Gainesville, Florida, 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/S0011-8532(22)00816-3","article-title":"Microbiologic aspects of dental plaque and dental caries","volume":"43","author":"Marsh","year":"1999","journal-title":"Dent Clin North Am"},{"key":"R2","first-page":"411","volume-title":"Molecular Biology of Streptococci Horizon Bioscience","author":"Schachtele","year":"2007"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.GPP3-0051-2018"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1089\/ast.2009.0415"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.14219\/jada.archive.1976.0502"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-020-00830-z"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1038\/s41526-016-0006-4"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1038\/s41526-024-00427-y"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0170826"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1038\/s41526-018-0061-0"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-32818-z"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1089\/ast.2020.2235"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1677-2_18"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.5.1374-1382.2000"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1550-8_10"},{"key":"R16","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1007\/978-1-4419-9863-7_1215","volume-title":"Encyclopedia of Systems Biology","author":"Haynes","year":"2013"},{"key":"R17","article-title":"Venny. An interactive tool for comparing lists with Venn\u2019s diagrams. 2007\u20132015","author":"Venny"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac352"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz931"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1099"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(62)90050-x"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1128\/iai.12.3.475-479.1975"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00425-09"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1038\/s44260-025-00068-6"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-022-12045-3"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.01279"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2015.04.004"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1128\/jb.00115-25"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01447-14"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00321"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1038\/npjmgrav.2016.21"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062437"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707155104"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.68.2.345-361.2004"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080677"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.3390\/life11040283"},{"key":"R37","first-page":"71","article-title":"A brief history of aerospace dentistry","volume":"50","author":"Savage","year":"2002","journal-title":"J Hist Dent"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.001776?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:16:56Z","timestamp":1791217016000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.001776"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,5]]},"references-count":37,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10,5]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.001776","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,5]]}}}