{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T09:47:51Z","timestamp":1791884871480,"version":"4.3.4"},"reference-count":50,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Ireland","award":["22\/PATH-S\/10678"],"award-info":[{"award-number":["22\/PATH-S\/10678"]}],"id":[{"id":"https:\/\/ror.org\/010t7sr36","id-type":"ROR","asserted-by":"crossref"}]},{"name":"UCD Foundation","id":[{"id":"https:\/\/ror.org\/02yc19841","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Research Ireland","award":["FRL4862"],"award-info":[{"award-number":["FRL4862"]}],"id":[{"id":"https:\/\/ror.org\/010t7sr36","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Research Ireland","award":["IRCLA\/2022\/3619"],"award-info":[{"award-number":["IRCLA\/2022\/3619"]}],"id":[{"id":"https:\/\/ror.org\/010t7sr36","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,9]]},"abstract":"<jats:p>\n                    Non-tuberculous mycobacterial (NTM) lung disease is now recognized as a chronic condition with considerable morbidity and mortality with incidence rising globally. This is concerning as these infections are often mis- or undiagnosed and currently available treatment regimens have lengthy (&gt;1\u2009year) treatment courses with variable to poor success rates. Identification of vulnerable targets and pathways in NTM pathogens is urgently needed to develop improved therapeutics. Itaconic acid (ITA), a macrophage-produced metabolite with immunomodulatory and antibacterial activity, inhibits growth of mycobacteria and here, we provide further important insights into the activity and mechanism of action for ITA. ITA displayed robust bactericidal activity against the prominent NTM pathogens\n                    <jats:italic>Mycobacterium avium<\/jats:italic>\n                    (\n                    <jats:italic>M. avium<\/jats:italic>\n                    ) and\n                    <jats:italic>M. abscessus<\/jats:italic>\n                    in a pH- and fatty acid-dependent manner, conditions that these bacteria experience within phagosomes during infection. Using a genetically encoded intrabacterial pH reporter, we demonstrate that ITA induces intracellular acidification in a pH-dependent manner. This work reveals that disruption of NTM pH homeostasis is a vulnerability in these difficult-to-treat pathogens which could be exploited for therapeutic benefit.\n                  <\/jats:p>","DOI":"10.1099\/mic.0.001782","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:16:56Z","timestamp":1791217016000},"source":"Crossref","is-referenced-by-count":0,"title":["Fatty acid-dependent cidality in Mycobacterium avium and Mycobacterium abscessus: intracellular acidification by itaconic acid"],"prefix":"10.1099","volume":"172","author":[{"given":"Hannah","family":"Lynch","sequence":"first","affiliation":[{"name":"School of Medicine, Trinity College Dublin, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronan","family":"Murray","sequence":"additional","affiliation":[{"name":"School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donal J","family":"Cox","sequence":"additional","affiliation":[{"name":"Trinity Translational Medicine Institute, St James\u2019s Hospital, Trinity College Dublin, Dublin, Ireland"},{"name":"School of Medicine, Trinity College Dublin, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam J","family":"Byrne","sequence":"additional","affiliation":[{"name":"Conway Institute, University College Dublin, Dublin 4, Ireland"},{"name":"School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cormac","family":"McCarthy","sequence":"additional","affiliation":[{"name":"St. Vincent\u2019s University Hospital, Dublin, Ireland"},{"name":"Conway Institute, University College Dublin, Dublin 4, Ireland"},{"name":"School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne","family":"Cloonan","sequence":"additional","affiliation":[{"name":"Discipline of Clinical Medicine, Trinity Centre for Health Sciences, Tallaght University Hospital, Dublin, Ireland"},{"name":"Division of Pulmonary and Critical Care Medicine, Department of Medicine, Joan and Sanford I. Weill, New York, NY, USA"},{"name":"School of Medicine, Trinity College Dublin, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6903-2455","authenticated-orcid":false,"given":"Claire","family":"Healy","sequence":"additional","affiliation":[{"name":"Conway Institute, University College Dublin, Dublin 4, Ireland"},{"name":"School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland"},{"name":"School of Medicine, Trinity College Dublin, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200604-571ST"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1660864"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201804-236OC"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf8156"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007083"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1183\/20734735.0171-2021"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa241"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/S1995-7645(10)60094-6"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005408"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-017-8192-x"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1172\/JCI148548"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1021\/pr101054m"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2423114122"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02121-21"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00104-22"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1218599110"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2024571118"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00303-20"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0303516"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1795"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1002\/2211-5463.13944"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2006.05.005"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00402-19"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2025.1545856"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1038\/jid.1953.18"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-020-0210-0"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1054\/tube.2002.0335"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00069"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.23.7161-7167.1999"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1038\/35021074"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1128\/jb.131.1.136-144.1977"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.001050"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.160.3.1290"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.1999.tb00660.x"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1038\/nm1252"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.abc1884"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2021.100277"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1111\/all.70107"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12092"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.43621"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1126\/science.182.4118.1258"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(93)81542-X"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.084343"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00013-14"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaz1333"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-65582-6"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg287"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1101\/2025.09.26.678883"},{"key":"R49","doi-asserted-by":"publisher","DOI":"10.64898\/2026.03.10.710584"},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1164\/arrd.1986.134.6.1287"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.001782?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T09:16:37Z","timestamp":1791882997000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.001782"}},"subtitle":["&lt;xhtml:span xmlns:xhtml=\"http:\/\/www.w3.org\/1999\/xhtml\" xml:lang=\"en\"&gt;&lt;p&gt;This article is part of our &lt;span style=\"color: rgb(33, 51, 104);\"&gt;&lt;a href=\"\/content\/emerging-investigators-collection-2025-26\"&gt;Emerging Investigators collection&lt;\/a&gt;&lt;\/span&gt;.&lt;\/p&gt;&lt;p style=\"line-height: 1.2;\"&gt;&lt;img src=\"\/upload\/emerging\/Claire_Healey.png\" alt=\"photograph of Claire Healy\" class=\"ImageBorder img-responsive eb-people fr-fic fr-dib\" style=\"width: 120px; float: left; margin: 0 10px 10px 0;\" \/&gt;&lt;span style=\"font-size: 12px; font-weight: normal;\"&gt;&lt;strong&gt;Claire Healy&lt;\/strong&gt; is an Assistant Professor and Ad Astra Fellow at University College Dublin. She obtained her PhD in Infection Biology at University College Dublin before conducting postdoctoral research at Weill Cornell Medicine, USA, where she investigated the molecular mechanisms by which &lt;em&gt;Mycobacterium tuberculosis&lt;\/em&gt;, the causative agent of tuberculosis, persists in the host using several complementary approaches, including genome-wide mutant fitness profiling, transcriptomics, metabolomics and murine infection models. As a Research Fellow at Trinity College Dublin, Dr Healy broadened her expertise to pathogen-host interactions, particularly in the context of chronic lung disease. Her research is now focused on the emerging non-tuberculous mycobacterial (NTM) pathogens &lt;em&gt;M. avium&amp;#160;&lt;\/em&gt;and &lt;em&gt;M. abscessus.&lt;\/em&gt; Her groups employs molecular microbiology and mycobacterial genetics approaches, as well as &lt;em&gt;in vitro\/ex vivo&lt;\/em&gt; macrophage infection models to investigate the role of host-derived nutrients on bacterial pathogenesis and host response during opportunistic NTM infection&lt;\/span&gt;&lt;\/p&gt;\n&lt;\/xhtml:span&gt;"],"short-title":[],"issued":{"date-parts":[[2026,10,9]]},"references-count":50,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10,9]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.001782","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,9]]}}}