{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T03:05:23Z","timestamp":1777518323747,"version":"3.51.4"},"reference-count":42,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,3,1]]},"abstract":"<jats:p><jats:italic>Listeria monocytogenes<\/jats:italic>is a foodborne pathogen able to infect humans and many other mammalian species, leading to serious, often fatal disease. We have previously identified a five-gene locus in the genome of<jats:italic>L. monocytogenes<\/jats:italic>EGD-e which comprised three contiguous genes encoding paralogous type I signal peptidases. In the present study, we focused on the two distal genes of the locus (<jats:italic>lmo1272<\/jats:italic>and<jats:italic>lmo1273<\/jats:italic>), encoding proteins sharing significant similarities with the YlqF and RnhB proteins, respectively, of<jats:italic>Bacillus subtilis<\/jats:italic>.<jats:italic>lmo1273<\/jats:italic>could complement an<jats:italic>Escherichia coli rnhA-rnhB<\/jats:italic>thermosensitive growth phenotype, suggesting that it encodes a functional RNase H. Strikingly, inactivation of<jats:italic>lmo1273<\/jats:italic>provoked a strong attenuation of virulence in the mouse model, and kinetic studies in infected mice revealed that multiplication of the<jats:italic>lmo1273<\/jats:italic>mutant in target organs was significantly impaired. However, the mutation did not impair<jats:italic>L. monocytogenes<\/jats:italic>intracellular multiplication or cell-to-cell spread in cell culture models. Transcriptional profiles obtained with an<jats:italic>lmo1273<\/jats:italic>-overexpressing strain were compared to those of the wild-type strain, using microarray analyses. The data obtained suggest a pleiotropic regulatory role of Lmo1273 and possible links with amino acid uptake.<\/jats:p>","DOI":"10.1099\/mic.0.022277-0","type":"journal-article","created":{"date-parts":[[2009,2,26]],"date-time":"2009-02-26T22:56:47Z","timestamp":1235689007000},"page":"891-902","source":"Crossref","is-referenced-by-count":7,"title":["lmo1273, a novel gene involved in Listeria monocytogenes virulence"],"prefix":"10.1099","volume":"155","author":[{"given":"Armelle","family":"Bigot","sequence":"first","affiliation":[{"name":"Inserm, U570, Unit\u00e9 de Pathog\u00e9nie des Infections Syst\u00e9miques, Paris F-75015, France"},{"name":"Universit\u00e9 Paris Descartes, Facult\u00e9 de M\u00e9decine Ren\u00e9 Descartes, Paris F-75015, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Raynaud","sequence":"additional","affiliation":[{"name":"Inserm, U570, Unit\u00e9 de Pathog\u00e9nie des Infections Syst\u00e9miques, Paris F-75015, 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