{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T02:54:14Z","timestamp":1792032854403,"version":"4.4.0"},"reference-count":75,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,4,1]]},"abstract":"<jats:p>Bacterial spores possess a series of concentrically arranged protective structures that contribute to dormancy, survival and, ultimately, germination. One of these structures, the coat, is present in all spores. In<jats:italic>Bacillus anthracis<\/jats:italic>, however, the spore is surrounded by an additional, poorly understood, morphologically complex structure called the exosporium. Here, we characterize three previously discovered exosporium proteins called ExsFA (also known as BxpB), ExsFB (a highly related paralogue of<jats:italic>exsFA\/bxpB<\/jats:italic>) and IunH (similar to an inosine\u2013uridine-preferring nucleoside hydrolase). We show that in the absence of ExsFA\/BxpB, the exosporium protein BclA accumulates asymmetrically to the forespore pole closest to the midpoint of the sporangium (i.e. the mother-cell-proximal pole of the forespore), instead of uniformly encircling the exosporium. ExsFA\/BxpB may also have a role in coat assembly, as mutant spore surfaces lack ridges seen in wild-type spores and have a bumpy appearance. ExsFA\/BxpB also has a modest but readily detected effect on germination. Nonetheless, an<jats:italic>exsFA\/bxpB<\/jats:italic>mutant strain is fully virulent in both intramuscular and aerosol challenge models in Guinea pigs. We show that the pattern of localization of ExsFA\/BxpB\u2013GFP is a ring, consistent with a location for this protein in the basal layer of the exosporium. In contrast, ExsFB\u2013GFP fluorescence is a solid oval, suggesting a distinct subcellular location for ExsFB\u2013GFP. We also used these fusion proteins to monitor changes in the subcellular locations of these proteins during sporulation. Early in sporulation, both fusions were present throughout the mother cell cytoplasm. As sporulation progressed, GFP fluorescence moved from the mother cell cytoplasm to the forespore surface and formed either a ring of fluorescence, in the case of ExsFA\/BxpB, or a solid oval of fluorescence, in the case of ExsFB. IunH\u2013GFP also resulted in a solid oval of fluorescence. We suggest the interpretation that at least some ExsFB\u2013GFP and IunH\u2013GFP resides in the region between the coat and the exosporium, called the interspace.<\/jats:p>","DOI":"10.1099\/mic.0.023333-0","type":"journal-article","created":{"date-parts":[[2009,3,31]],"date-time":"2009-03-31T07:52:51Z","timestamp":1238485971000},"page":"1133-1145","source":"Crossref","is-referenced-by-count":49,"title":["Localization and assembly of proteins comprising the outer structures of the Bacillus anthracis spore"],"prefix":"10.1099","volume":"155","author":[{"given":"Rebecca","family":"Giorno","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Loyola University Medical Center, Maywood, IL 60153, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Mallozzi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Loyola University Medical Center, Maywood, IL 60153, 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Bacteriol"},{"key":"R7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.micpath.2004.10.001","article-title":"Construction of a rhamnose mutation in Bacillus anthracis affects adherence to macrophages but not virulence in guinea pigs","volume":"38","author":"Bozue","year":"2005","journal-title":"Microb Pathog"},{"key":"R8","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1128\/IAI.01202-06","article-title":"Fully virulent Bacillus anthracis does not require the immunodominant protein BclA for pathogenesis","volume":"75","author":"Bozue","year":"2007a","journal-title":"Infect Immun"},{"key":"R9","doi-asserted-by":"crossref","first-page":"4498","DOI":"10.1128\/IAI.00434-07","article-title":"Bacillus anthracis spores of the bclA mutant exhibit increased adherence to epithelial, fibroblast, and endothelial cells but not macrophages","volume":"75","author":"Bozue","year":"2007b","journal-title":"Infect 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anthracis live spore vaccine and protective antigen vaccine against anthrax in the guinea pig","volume":"52","author":"Little","year":"1986","journal-title":"Infect Immun"},{"key":"R38","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1146\/annurev.ge.20.120186.003205","article-title":"Genetics of endospore formation in Bacillus subtilis","volume":"20","author":"Losick","year":"1986","journal-title":"Annu Rev Genet"},{"key":"R39","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1111\/j.1574-6968.2008.01380.x","article-title":"Characterization of a Bacillus anthracis spore coat-surface protein that influences coat-surface morphology","volume":"289","author":"Mallozzi","year":"2008","journal-title":"FEMS Microbiol Lett"},{"key":"R40","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1214\/aoms\/1177730491","article-title":"On a test of whether one of two random variables is stochastically larger than the 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Bacteriol"},{"key":"R44","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.micpath.2004.06.008","article-title":"The NheA component of the non-hemolytic enterotoxin of Bacillus cereus is produced by Bacillus anthracis but is not required for virulence","volume":"37","author":"Mendelson","year":"2004","journal-title":"Microb Pathog"},{"key":"R45","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1146\/annurev.micro.55.1.647","article-title":"Anthrax","volume":"55","author":"Mock","year":"2001","journal-title":"Annu Rev Microbiol"},{"key":"R46","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/s00018-002-8432-8","article-title":"Spore germination","volume":"59","author":"Moir","year":"2002","journal-title":"Cell Mol Life Sci"},{"key":"R47","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1007\/s00018-002-8433-7","article-title":"Roles of Bacillus endospores in the environment","volume":"59","author":"Nicholson","year":"2002","journal-title":"Cell Mol Life Sci"},{"key":"R48","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1128\/JB.115.3.1179-1190.1973","article-title":"Exosporium and spore coat formation in Bacillus cereus T","volume":"115","author":"Ohye","year":"1973","journal-title":"J Bacteriol"},{"key":"R49","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1073\/pnas.0709321105","article-title":"The integrin Mac-1 (CR3) mediates internalization and directs Bacillus anthracis spores into professional phagocytes","volume":"105","author":"Oliva","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R50","doi-asserted-by":"crossref","first-page":"2617","DOI":"10.1128\/JB.173.8.2617-2624.1991","article-title":"Mry, a trans -acting positive regulator of the M protein gene of Streptococcus pyogenes with similarity to the receptor proteins of two-component regulatory systems","volume":"173","author":"Perez-Casal","year":"1991","journal-title":"J Bacteriol"},{"key":"R51","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1529\/biophysj.104.049312","article-title":"The high-resolution architecture and structural dynamics of Bacillus spores","volume":"88","author":"Plomp","year":"2004","journal-title":"Biophys J"},{"key":"R52","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1111\/j.1365-2958.1995.mmi_18030459.x","article-title":"Visualization of the subcellular location of sporulation proteins in Bacillus subtilis using immunofluorescence microscopy","volume":"18","author":"Pogliano","year":"1995","journal-title":"Mol Microbiol"},{"key":"R53","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1007\/s00018-002-8435-5","article-title":"Specialized peptidoglycan of the bacterial endospore: the inner wall of the lockbox","volume":"59","author":"Popham","year":"2002","journal-title":"Cell Mol Life 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