{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T14:34:10Z","timestamp":1718030050925},"reference-count":74,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,8,1]]},"abstract":"<jats:p>\n                  <jats:italic>Paracoccidioides brasiliensis<\/jats:italic> is a thermally dimorphic fungus, and causes the most prevalent systemic mycosis in Latin America. Infection is initiated by inhalation of conidia or mycelial fragments by the host, followed by further differentiation into the yeast form. Information regarding gene expression by either form has rarely been addressed with respect to multiple time points of growth in culture. Here, we report on the construction of a genomic DNA microarray, covering approximately 25\u200a% of the genome of the organism, and its utilization in identifying genes and gene expression patterns during growth <jats:italic>in vitro<\/jats:italic>. Cloned, amplified inserts from randomly sheared genomic DNA (gDNA) and known control genes were printed onto glass slides to generate a microarray of over 12\u200a000 elements. To examine gene expression, mRNA was extracted and amplified from mycelial or yeast cultures grown in semi-defined medium for 5, 8 and 14\u2005days. Principal components analysis and hierarchical clustering indicated that yeast gene expression profiles differed greatly from those of mycelia, especially at earlier time points, and that mycelial gene expression changed less than gene expression in yeasts over time. Genes upregulated in yeasts were found to encode proteins shown to be involved in methionine\/cysteine metabolism, respiratory and metabolic processes (of sugars, amino acids, proteins and lipids), transporters (small peptides, sugars, ions and toxins), regulatory proteins and transcription factors. Mycelial genes involved in processes such as cell division, protein catabolism, nucleotide biosynthesis and toxin and sugar transport showed differential expression. Sequenced clones were compared with <jats:italic>Histoplasma capsulatum<\/jats:italic> and <jats:italic>Coccidioides posadasii<\/jats:italic> genome sequences to assess potentially common pathways across species, such as sulfur and lipid metabolism, amino acid transporters, transcription factors and genes possibly related to virulence. We also analysed gene expression with time in culture and found that while transposable elements and components of respiratory pathways tended to increase in expression with time, genes encoding ribosomal structural proteins and protein catabolism tended to sharply decrease in expression over time, particularly in yeast. These findings expand our knowledge of the different morphological forms of <jats:italic>P. brasiliensis<\/jats:italic> during growth in culture.<\/jats:p>","DOI":"10.1099\/mic.0.027441-0","type":"journal-article","created":{"date-parts":[[2009,7,29]],"date-time":"2009-07-29T01:51:17Z","timestamp":1248832277000},"page":"2795-2808","source":"Crossref","is-referenced-by-count":26,"title":["Genomic DNA microarray comparison of gene expression patterns in Paracoccidioides brasiliensis mycelia and yeasts in vitro"],"prefix":"10.1099","volume":"155","author":[{"given":"Jomar Patr\u00edcio","family":"Monteiro","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Santa Clara Valley Medical Center, San Jose, CA, USA"},{"name":"Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA, USA"},{"name":"California Institute for Medical Research, San Jose, CA, USA"},{"name":"Genetics Department, Biosciences Institute, UNESP, Botucatu, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl V.","family":"Clemons","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA, USA"},{"name":"Department of Medicine, Division of Infectious Diseases, Santa Clara Valley Medical Center, San Jose, CA, USA"},{"name":"California Institute for Medical Research, San Jose, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurence F.","family":"Mirels","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA, USA"},{"name":"Department of Medicine, Division of Infectious Diseases, Santa Clara Valley Medical Center, San Jose, CA, USA"},{"name":"California Institute for Medical Research, San Jose, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John A.","family":"Coller","sequence":"additional","affiliation":[{"name":"Stanford Functional Genomics Facility, Stanford University, Stanford, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas D.","family":"Wu","sequence":"additional","affiliation":[{"name":"Bioinformatics Department, Genentech, Inc., South San Francisco, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jata","family":"Shankar","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA, USA"},{"name":"Department of Medicine, Division of Infectious Diseases, Santa Clara Valley Medical Center, San Jose, CA, USA"},{"name":"California Institute for Medical Research, San Jose, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catalina 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