{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T09:53:43Z","timestamp":1789984423820,"version":"4.0.1"},"reference-count":45,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,9,1]]},"abstract":"<jats:p>\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    is a well-known pathogen in chronic respiratory diseases such as cystic fibrosis. Infectivity of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    is related to the ability to grow under oxygen-limited conditions using the anaerobic metabolism of denitrification, in which nitrate is reduced to dinitrogen via nitric oxide (NO). Denitrification is activated by a cascade of redox-sensitive transcription factors, among which is the DNR regulator, sensitive to nitrogen oxides. To gain further insight into the mechanism of NO-sensing by DNR, we have developed an\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    -based reporter system to investigate different aspects of DNR activity. In\n                    <jats:italic>E. coli<\/jats:italic>\n                    DNR responds to NO, as shown by its ability to transactivate the\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    <jats:italic>norCB<\/jats:italic>\n                    promoter. The direct binding of DNR to the target DNA is required, since mutations in the helix\u2013turn\u2013helix domain of DNR and specific nucleotide substitutions in the consensus sequence of the\n                    <jats:italic>norCB<\/jats:italic>\n                    promoter abolish the transcriptional activity. Using an\n                    <jats:italic>E. coli<\/jats:italic>\n                    strain deficient in haem biosynthesis, we have also confirmed that haem is required\n                    <jats:italic>in vivo<\/jats:italic>\n                    for the NO-dependent DNR activity, in agreement with the property of DNR to bind haem\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Finally, we have shown, we believe for the first time, that DNR is able to discriminate\n                    <jats:italic>in vivo<\/jats:italic>\n                    between different diatomic signal molecules, NO and CO, both ligands of the reduced haem iron\n                    <jats:italic>in vitro<\/jats:italic>\n                    , suggesting that DNR responds specifically to NO.\n                  <\/jats:p>","DOI":"10.1099\/mic.0.028027-0","type":"journal-article","created":{"date-parts":[[2009,8,31]],"date-time":"2009-08-31T18:45:30Z","timestamp":1251744330000},"page":"2838-2844","source":"Crossref","is-referenced-by-count":54,"title":["The transcription factor DNR from Pseudomonas aeruginosa specifically requires nitric oxide and haem for the activation of a target promoter in Escherichia coli"],"prefix":"10.1099","volume":"155","author":[{"given":"Nicoletta","family":"Castiglione","sequence":"first","affiliation":[{"name":"Department of Biochemical Sciences \u2018A. Rossi Fanelli\u2019, Sapienza University of Rome, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena","family":"Rinaldo","sequence":"additional","affiliation":[{"name":"Department of Biochemical Sciences \u2018A. Rossi Fanelli\u2019, Sapienza University of Rome, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio","family":"Giardina","sequence":"additional","affiliation":[{"name":"Department of Biochemical Sciences \u2018A. Rossi Fanelli\u2019, Sapienza University of Rome, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Cutruzzol\u00e0","sequence":"additional","affiliation":[{"name":"Consorzio INBB, 00136 Rome, Italy"},{"name":"Department of Biochemical Sciences \u2018A. 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