{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:03:29Z","timestamp":1790697809409,"version":"4.1.0"},"reference-count":36,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,8,1]]},"abstract":"<jats:p>\n                  <jats:italic>Pseudomonas aeruginosa<\/jats:italic> frequently acts as an opportunistic pathogen of mucosal surfaces; yet, despite causing aggressive prostatitis in some men, its role as a pathogen in the prostate has not been investigated. Consequently, we developed a <jats:italic>Ps. aeruginosa<\/jats:italic> infection model in the rat prostate by instilling wild-type (WT) <jats:italic>Ps. aeruginosa<\/jats:italic> strain PAO1 into the rat prostate. It was found that <jats:italic>Ps. aeruginosa<\/jats:italic> produced acute and chronic infections in this mucosal tissue as determined by bacterial colonization, gross morphology, tissue damage and inflammatory markers. WT strain PAO1 and its isogenic mutant PAO-JP2, in which both the <jats:italic>lasI<\/jats:italic> and <jats:italic>rhlI<\/jats:italic> quorum-sensing signal systems have been silenced, were compared during both acute and chronic prostate infections. In acute infections, bacterial numbers and inflammatory markers were comparable between WT PA01 and PAO-JP2; however, considerably less tissue damage occurred in infections with PAO-JP2. Chronic infections with PAO-JP2 resulted in reduced bacterial colonization, tissue damage and inflammation as compared to WT PAO1 infections. Therefore, the quorum-sensing <jats:italic>lasI<\/jats:italic> and <jats:italic>rhlI<\/jats:italic> genes in <jats:italic>Ps. aeruginosa<\/jats:italic> affect acute prostate infections, but play a considerably more important role in maintaining chronic infections. We have thus developed a highly reproducible model for the study of <jats:italic>Ps. aeruginosa<\/jats:italic> virulence in the prostate.<\/jats:p>","DOI":"10.1099\/mic.0.028464-0","type":"journal-article","created":{"date-parts":[[2009,7,29]],"date-time":"2009-07-29T01:51:17Z","timestamp":1248832277000},"page":"2612-2619","source":"Crossref","is-referenced-by-count":34,"title":["Pseudomonas aeruginosa las and rhl quorum-sensing systems are important for infection and inflammation in a rat prostatitis model"],"prefix":"10.1099","volume":"155","author":[{"given":"Lisa K.","family":"Nelson","sequence":"first","affiliation":[{"name":"Biofilm Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevieve H.","family":"D'Amours","sequence":"additional","affiliation":[{"name":"Biofilm Research Group, Department of Biological Sciences, University of 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