{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T19:54:15Z","timestamp":1790798055592,"version":"4.1.0"},"reference-count":73,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,12,1]]},"abstract":"<jats:p>\n                  <jats:italic>Klebsiella pneumoniae<\/jats:italic> is an enteric pathogen causing community-acquired and hospital-acquired infections in humans. Epidemiological studies have revealed significant diversity in capsular polysaccharide (CPS) type and clinical manifestation of <jats:italic>K. pneumoniae<\/jats:italic> infection in different geographical areas of the world. We have sequenced the capsular polysaccharide synthesis (<jats:italic>cps<\/jats:italic>) region of seven clinical isolates and compared the sequences with the publicly available <jats:italic>cps<\/jats:italic> sequence data of five strains: NTUH-K2044 (K1 serotype), Chedid (K2 serotype), MGH78578 (K52 serotype), A1142 (K57 serotype) and A1517. Among all strains, six genes at the 5\u2032 end of the <jats:italic>cps<\/jats:italic> clusters that encode proteins for CPS transportation and processing at the bacterial surface are highly similar to each other. The central region of the <jats:italic>cps<\/jats:italic> gene clusters, which encodes proteins for polymerization and assembly of the CPS subunits, is highly divergent. Based on the collected sequence, we found that either the <jats:italic>wbaP<\/jats:italic> gene or the <jats:italic>wcaJ<\/jats:italic> gene exists in a given <jats:italic>K. pneumoniae<\/jats:italic> strain, suggesting that there is a major difference in the CPS biosynthesis pathway and that the <jats:italic>K. pneumoniae<\/jats:italic> strains can be classified into at least two distinct groups. All isolates contain <jats:italic>gnd<\/jats:italic>, encoding gluconate-6-phosphate dehydrogenase, at the 3\u2032 end of the <jats:italic>cps<\/jats:italic> gene clusters. The <jats:italic>rmlBADC<\/jats:italic> genes were found in CPS K9-positive, K14-positive and K52-positive strains, while <jats:italic>manC<\/jats:italic> and <jats:italic>manB<\/jats:italic> were found in K1, K2, K5, K14, K62 and two undefined strains. Our data indicate that, while overall genomic organization is similar between different pathogenic <jats:italic>K. pneumoniae<\/jats:italic> strains, the genetic variation of the sugar moiety and polysaccharide linkage generate the diversity in CPS molecules that could help evade host immune attack.<\/jats:p>","DOI":"10.1099\/mic.0.029017-0","type":"journal-article","created":{"date-parts":[[2009,11,3]],"date-time":"2009-11-03T22:52:21Z","timestamp":1257288741000},"page":"4170-4183","source":"Crossref","is-referenced-by-count":110,"title":["Genetic diversity of capsular polysaccharide biosynthesis in Klebsiella pneumoniae clinical isolates"],"prefix":"10.1099","volume":"155","author":[{"given":"Hung-Yu","family":"Shu","sequence":"first","affiliation":[{"name":"Genome Research Center, National Yang-Ming University, Taipei, Taiwan, ROC"},{"name":"Department of Bioscience Technology, Chang Jung Christian University, Tainan County, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-Phone","family":"Fung","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC"},{"name":"Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yen-Ming","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan, Miaoli County, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keh-Ming","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Informatics, National Yang-Ming University, Taipei, Taiwan, ROC"},{"name":"Division of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan, Miaoli County, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Tsong","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan, Miaoli County, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling-Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan, Miaoli County, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tze-Tze","family":"Liu","sequence":"additional","affiliation":[{"name":"Genome Research Center, National Yang-Ming University, Taipei, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph","family":"Kirby","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih-Feng","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan, ROC"},{"name":"Division of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan, Miaoli County, Taiwan, ROC"},{"name":"Genome Research Center, National Yang-Ming University, Taipei, Taiwan, ROC"},{"name":"Institute of Biomedical Informatics, National Yang-Ming University, Taipei, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1093\/glycob\/10.9.875","article-title":"Preparative synthesis of GDP- \u03b2-l-fucose by recombinant enzymes from enterobacterial sources","volume":"10","author":"Albermann","year":"2000","journal-title":"Glycobiology"},{"key":"R2","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped blast and psi-blast: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res"},{"key":"R3","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1128\/JB.180.4.998-1001.1998","article-title":"Identification of the fucose synthetase gene in the colanic acid gene cluster of Escherichia coli K-12","volume":"180","author":"Andrianopoulos","year":"1998","journal-title":"J Bacteriol"},{"key":"R4","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1128\/JB.177.7.1788-1796.1995","article-title":"Genomic organization of the Klebsiella pneumoniae cps region responsible for serotype K2 capsular polysaccharide synthesis in the virulent strain Chedid","volume":"177","author":"Arakawa","year":"1995","journal-title":"J Bacteriol"},{"key":"R5","doi-asserted-by":"crossref","first-page":"D138","DOI":"10.1093\/nar\/gkh121","article-title":"The Pfam protein families database","volume":"32","author":"Bateman","year":"2004","journal-title":"Nucleic Acids Res"},{"key":"R6","doi-asserted-by":"crossref","first-page":"28227","DOI":"10.1074\/jbc.M402913200","article-title":"Three-dimensional structure of Wza, the protein required for translocation of group 1 capsular polysaccharide across the outer membrane of Escherichia coli","volume":"279","author":"Beis","year":"2004","journal-title":"J Biol Chem"},{"key":"R7","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/0097-8485(93)85004-V","article-title":"genmark: Parallel gene recognition for both DNA strands","volume":"17","author":"Borodovsky","year":"1993","journal-title":"Comput Chem"},{"key":"R8","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1093\/oxfordjournals.jbchem.a022035","article-title":"Sequence\u2013function relationships of prokaryotic and eukaryotic galactosyltransferases","volume":"123","author":"Breton","year":"1998","journal-title":"J Biochem"},{"key":"R9","doi-asserted-by":"crossref","first-page":"3388","DOI":"10.1128\/JCM.42.8.3388-3398.2004","article-title":"Molecular serotyping of Klebsiella species isolates by restriction of the amplified capsular antigen gene cluster","volume":"42","author":"Brisse","year":"2004","journal-title":"J Clin Microbiol"},{"key":"R10","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/0008-6215(95)00181-R","article-title":"The structure of the acidic exopolysaccharide produced by Pseudomonas \u201cgingeri\u201d strain Pf9","volume":"275","author":"Cescutti","year":"1995","journal-title":"Carbohydr Res"},{"key":"R11","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1128\/JB.141.2.971-972.1980","article-title":"Primary structure of the Escherichia coli serotype K30 capsular polysaccharide","volume":"141","author":"Chakraborty","year":"1980","journal-title":"J Bacteriol"},{"key":"R12","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1086\/499968","article-title":"Genetic determinants of capsular serotype K1 of Klebsiella pneumoniae causing primary pyogenic liver abscess","volume":"193","author":"Chuang","year":"2006","journal-title":"J Infect Dis"},{"key":"R13","doi-asserted-by":"crossref","first-page":"2144","DOI":"10.1074\/jbc.M508078200","article-title":"Periplasmic protein\u2013protein contacts in the inner membrane protein Wzc form a tetrameric complex required for the assembly of Escherichia coli group 1 capsules","volume":"281","author":"Collins","year":"2006","journal-title":"J Biol Chem"},{"key":"R14","doi-asserted-by":"crossref","first-page":"2212","DOI":"10.1016\/j.carres.2005.07.006","article-title":"1H and 13C NMR characterization and secondary structure of the K2 polysaccharide of Klebsiella pneumoniae strain 52145","volume":"340","author":"Corsaro","year":"2005","journal-title":"Carbohydr Res"},{"key":"R15","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0022-2836(03)00307-3","article-title":"An evolving hierarchical family classification for glycosyltransferases","volume":"328","author":"Coutinho","year":"2003","journal-title":"J Mol Biol"},{"key":"R16","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1111\/j.1574-6968.2008.01295.x","article-title":"Membrane topology of the Salmonella enterica serovar Typhimurium group B O-antigen translocase Wzx","volume":"287","author":"Cunneen","year":"2008","journal-title":"FEMS Microbiol Lett"},{"key":"R17","doi-asserted-by":"crossref","first-page":"4919","DOI":"10.1128\/AEM.71.8.4919-4924.2005","article-title":"Development of PCR assays targeting genes in O-antigen gene clusters for detection and identification of Escherichia coli O45 and O55 serogroups","volume":"71","author":"DebRoy","year":"2005","journal-title":"Appl Environ Microbiol"},{"key":"R18","doi-asserted-by":"crossref","first-page":"4636","DOI":"10.1093\/nar\/27.23.4636","article-title":"Improved microbial gene identification with GLIMMER","volume":"27","author":"Delcher","year":"1999","journal-title":"Nucleic Acids Res"},{"key":"R19","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1038\/nature05267","article-title":"Wza the translocon for E. coli capsular polysaccharides defines a new class of membrane protein","volume":"444","author":"Dong","year":"2006","journal-title":"Nature"},{"key":"R20","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1046\/j.1365-2958.1999.01277.x","article-title":"Gene products required for surface expression of the capsular form of the group 1 K antigen in Escherichia coli (O9a\u200a:\u200aK30","volume":"31","author":"Drummelsmith","year":"1999","journal-title":"Mol Microbiol"},{"key":"R21","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1101\/gr.8.3.186","article-title":"Base-calling of automated sequencer traces using phred. II. Error probabilities","volume":"8","author":"Ewing","year":"1998","journal-title":"Genome Res"},{"key":"R22","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1086\/519262","article-title":"Klebsiella pneumoniae genotype K1: an emerging pathogen that causes septic ocular or central nervous system complications from pyogenic liver abscess","volume":"45","author":"Fang","year":"2007","journal-title":"Clin Infect Dis"},{"key":"R23","doi-asserted-by":"crossref","first-page":"D247","DOI":"10.1093\/nar\/gkj149","article-title":"Pfam: clans, web tools and services","volume":"34","author":"Finn","year":"2006","journal-title":"Nucleic Acids Res"},{"key":"R24","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1093\/glycob\/cwg098","article-title":"Fold recognition analysis of glycosyltransferase families: further members of structural superfamilies","volume":"13","author":"Franco","year":"2003","journal-title":"Glycobiology"},{"key":"R25","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1086\/315488","article-title":"A 5-year study of the seroepidemiology of Klebsiella pneumoniae: high prevalence of capsular serotype K1 in Taiwan and implication for vaccine efficacy","volume":"181","author":"Fung","year":"2000","journal-title":"J Infect Dis"},{"key":"R26","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1136\/gut.50.3.420","article-title":"A global emerging disease of Klebsiella pneumoniae liver abscess: is serotype K1 an important factor for complicated endophthalmitis?","volume":"50","author":"Fung","year":"2002","journal-title":"Gut"},{"key":"R27","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/S0959-440X(00)00145-7","article-title":"The rhamnose pathway","volume":"10","author":"Giraud","year":"2000","journal-title":"Curr Opin Struct Biol"},{"key":"R28","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1101\/gr.8.3.195","article-title":"Consed: a graphical tool for sequence finishing","volume":"8","author":"Gordon","year":"1998","journal-title":"Genome Res"},{"key":"R29","doi-asserted-by":"crossref","first-page":"42566","DOI":"10.1074\/jbc.M401340200","article-title":"Analysis of the DXD motifs in human xylosyltransferase I required for enzyme activity","volume":"279","author":"Go\u0308tting","year":"2004","journal-title":"J Biol Chem"},{"key":"R30","doi-asserted-by":"crossref","first-page":"39323","DOI":"10.1074\/jbc.M305134200","article-title":"Autophosphorylation of the Escherichia coli protein kinase Wzc regulates tyrosine phosphorylation of Ugd, a UDP-glucose dehydrogenase","volume":"278","author":"Grangeasse","year":"2003","journal-title":"J Biol Chem"},{"key":"R31","doi-asserted-by":"crossref","first-page":"3708","DOI":"10.1128\/AEM.68.8.3708-3715.2002","article-title":"Homologs of the Rml enzymes from Salmonella enterica are responsible for dTDP- \u03b2-l-rhamnose biosynthesis in the Gram-positive thermophile Aneurinibacillus thermoaerophilus DSM 10155","volume":"68","author":"Graninger","year":"2002","journal-title":"Appl Environ Microbiol"},{"key":"R32","first-page":"166","article-title":"TMbase \u2013 a database of membrane spanning protein segments","volume":"374","author":"Hofmann","year":"1993","journal-title":"Biol Chem Hoppe Seyler"},{"key":"R33","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1139\/m88-204","article-title":"Monoclonal antibodies against the capsular K antigen of Escherichia coli (O9\u200a:\u200aK30(A)\u200a:\u200aH12): characterisation and use in analysis of K antigen organisation on the cell surface","volume":"34","author":"Homonylo","year":"1988","journal-title":"Can J Microbiol"},{"key":"R34","first-page":"113","article-title":"Capsules of Escherichia coli","volume-title":"Escherichia coli: Mechanisms of Virulence","author":"Jann","year":"1997"},{"key":"R35","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1128\/IAI.69.3.1244-1255.2001","article-title":"Molecular characterization of S treptococcus pneumoniae type 4, 6B, 8, and 18C capsular polysaccharide gene clusters","volume":"69","author":"Jiang","year":"2001","journal-title":"Infect Immun"},{"key":"R36","doi-asserted-by":"crossref","first-page":"17890","DOI":"10.1074\/jbc.M701941200","article-title":"A \u03b2-1,2-xylosyltransferase from Cryptococcus neoformans defines a new family of glycosyltransferases","volume":"282","author":"Klutts","year":"2007","journal-title":"J Biol Chem"},{"key":"R37","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1099\/jmm.0.45924-0","article-title":"A molecular-capsular-type prediction system for 90 Streptococcus pneumoniae serotypes using partial cpsA- cpsB sequencing and wzy- or wzx-specific PCR","volume":"54","author":"Kong","year":"2005","journal-title":"J Med Microbiol"},{"key":"R38","doi-asserted-by":"crossref","first-page":"7786","DOI":"10.1128\/JB.175.24.7786-7792.1993","article-title":"A 3.9-kb DNA region of Xanthomonas campestris pv. campestris that is necessary for lipopolysaccharide production encodes a set of enzymes involved in the synthesis of dTDP-rhamnose","volume":"175","author":"Ko\u0308plin","year":"1993","journal-title":"J Bacteriol"},{"key":"R39","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1139\/m88-173","article-title":"Transfer and expression of the genetic determinants for O and K antigen synthesis in Escherichia coli O9\u200a:\u200aK(A)30 and Klebsiella sp. O1\u200a:\u200aK20, in Escherichia coli K12","volume":"34","author":"Laakso","year":"1988","journal-title":"Can J Microbiol"},{"key":"R40","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1128\/JB.185.3.788-800.2003","article-title":"RmpA2, an activator of capsule biosynthesis in Klebsiella pneumoniae CG43, regulates K2 cps gene expression at the transcriptional level","volume":"185","author":"Lai","year":"2003","journal-title":"J Bacteriol"},{"key":"R41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/abio.1996.0419","article-title":"Enzymatic synthesis of UDP-galactofuranose and an assay for UDP-galactopyranose mutase based on high-performance liquid chromatography","volume":"242","author":"Lee","year":"1996","journal-title":"Anal Biochem"},{"key":"R42","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1093\/glycob\/11.3.217","article-title":"The DXD motif is required for GM2 synthase activity but is not critical for nucleotide binding","volume":"11","author":"Li","year":"2001","journal-title":"Glycobiology"},{"key":"R43","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1110\/ps.0302103","article-title":"Three monophyletic superfamilies account for the majority of the known glycosyltransferases","volume":"12","author":"Liu","year":"2003","journal-title":"Protein Sci"},{"key":"R44","doi-asserted-by":"crossref","first-page":"3408","DOI":"10.1128\/JB.175.11.3408-3413.1993","article-title":"Glycosyl transferases of O-antigen biosynthesis in Salmonella enterica: identification and characterization of transferase genes of groups B, C2, and E1","volume":"175","author":"Liu","year":"1993","journal-title":"J Bacteriol"},{"key":"R45","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1093\/nar\/26.4.1107","article-title":"GeneMark.hmm: new solutions for gene finding","volume":"26","author":"Lukashin","year":"1998","journal-title":"Nucleic Acids Res"},{"key":"R46","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1128\/AAC.45.5.1407-1416.2001","article-title":"Drug targeting Mycobacterium tuberculosis cell wall synthesis: genetics of dTDP-rhamnose synthetic enzymes and development of a microtiter plate-based screen for inhibitors of conversion of dTDP-glucose to dTDP-rhamnose","volume":"45","author":"Ma","year":"2001","journal-title":"Antimicrob Agents Chemother"},{"key":"R47","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1046\/j.1365-2958.1996.01531.x","article-title":"The GalF protein of Escherichia coli is not a UDP-glucose pyrophosphorylase but interacts with the GalU protein possibly to regulate cellular levels of UDP-glucose","volume":"22","author":"Marolda","year":"1996","journal-title":"Mol Microbiol"},{"key":"R48","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00203-005-0018-z","article-title":"Topological and transcriptional analysis of pssL gene product: a putative Wzx-like exopolysaccharide translocase in Rhizobium leguminosarum bv. trifolii TA1","volume":"184","author":"Mazur","year":"2005","journal-title":"Arch Microbiol"},{"key":"R49","volume-title":"Experiments in Molecular Genetics","author":"Miller","year":"1972"},{"key":"R50","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1111\/j.1348-0421.1989.tb00976.x","article-title":"Identification of species and capsular types of Klebsiella clinical isolates, with special reference to Klebsiella planticola","volume":"33","author":"Mori","year":"1989","journal-title":"Microbiol Immunol"},{"key":"R51","doi-asserted-by":"crossref","first-page":"5550","DOI":"10.1128\/IAI.00932-07","article-title":"Identification of a Wzy polymerase required for group IV capsular polysaccharide and lipopolysaccharide biosynthesis in Vibrio vulnificus","volume":"75","author":"Nakhamchik","year":"2007","journal-title":"Infect Immun"},{"key":"R52","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1128\/JB.178.4.1047-1052.1996","article-title":"Galactofuranose biosynthesis in Escherichia coli K-12: identification and cloning of UDP-galactopyranose mutase","volume":"178","author":"Nassau","year":"1996","journal-title":"J Bacteriol"},{"key":"R53","doi-asserted-by":"crossref","first-page":"10227","DOI":"10.1073\/pnas.91.21.10227","article-title":"Intergeneric transfer and recombination of the 6-phosphogluconate dehydrogenase gene ( gnd) in enteric bacteria","volume":"91","author":"Nelson","year":"1994","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R54","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1128\/JB.133.1.390-391.1978","article-title":"Primary structure of the Escherichia coli serotype K42 capsular polysaccharide and its serological identity with the Klebsiella K63 polysaccharide","volume":"133","author":"Niemann","year":"1978","journal-title":"J Bacteriol"},{"key":"R55","first-page":"195","article-title":"MacVector: an integrated sequence analysis program for the Macintosh","volume":"25","author":"Olson","year":"1994","journal-title":"Methods Mol Biol"},{"key":"R56","doi-asserted-by":"crossref","first-page":"2231","DOI":"10.1128\/JCM.01716-07","article-title":"Capsular polysaccharide synthesis regions in Klebsiella pneumoniae serotype K57 and a new capsular serotype","volume":"46","author":"Pan","year":"2008","journal-title":"J Clin Microbiol"},{"key":"R57","doi-asserted-by":"crossref","first-page":"9564","DOI":"10.1074\/jbc.M610998200","article-title":"Structural effects of naturally occurring human blood group B galactosyltransferase mutations adjacent to the DXD motif","volume":"282","author":"Persson","year":"2007","journal-title":"J Biol Chem"},{"key":"R58","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1128\/CMR.11.4.589","article-title":"Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors","volume":"11","author":"Podschun","year":"1998","journal-title":"Clin Microbiol Rev"},{"key":"R59","doi-asserted-by":"crossref","first-page":"D61","DOI":"10.1093\/nar\/gkl842","article-title":"NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins","volume":"35","author":"Pruitt","year":"2007","journal-title":"Nucleic Acids Res"},{"key":"R60","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1046\/j.1365-2958.2003.03354.x","article-title":"Transcriptional organization and regulation of the Escherichia coli K30 group 1 capsule biosynthesis ( cps) gene cluster","volume":"47","author":"Rahn","year":"2003","journal-title":"Mol Microbiol"},{"key":"R61","doi-asserted-by":"crossref","first-page":"2307","DOI":"10.1128\/JB.181.7.2307-2313.1999","article-title":"Conserved organization in the cps gene clusters for expression of Escherichia coli group 1 K antigens: relationship to the colanic acid biosynthesis locus and the cps genes from Klebsiella pneumoniae","volume":"181","author":"Rahn","year":"1999","journal-title":"J Bacteriol"},{"key":"R62","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1038\/nsb1001-858","article-title":"UDP-galactopyranose mutase has a novel structure and mechanism","volume":"8","author":"Sanders","year":"2001","journal-title":"Nat Struct Biol"},{"key":"R63","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0008-6215(97)00106-7","article-title":"The structure of the capsular polysaccharide from Klebsiella type 52, using the computerised approach CASPER and NMR spectroscopy","volume":"302","author":"Stenutz","year":"1997","journal-title":"Carbohydr Res"},{"key":"R64","doi-asserted-by":"crossref","first-page":"4885","DOI":"10.1128\/JB.178.16.4885-4893.1996","article-title":"Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid","volume":"178","author":"Stevenson","year":"1996","journal-title":"J Bacteriol"},{"key":"R65","doi-asserted-by":"crossref","first-page":"27274","DOI":"10.1074\/jbc.271.44.27274","article-title":"Synthesis of GDP-l-fucose by the human FX protein","volume":"271","author":"Tonetti","year":"1996","journal-title":"J Biol Chem"},{"key":"R66","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1006\/jmbi.1998.2107","article-title":"Principles governing amino acid composition of integral membrane proteins: application to topology prediction","volume":"283","author":"Tusna\u0301dy","year":"1998","journal-title":"J Mol Biol"},{"key":"R67","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1146\/annurev.biochem.75.103004.142545","article-title":"Biosynthesis and assembly of capsular polysaccharides in Escherichia coli","volume":"75","author":"Whitfield","year":"2006","journal-title":"Annu Rev Biochem"},{"key":"R68","doi-asserted-by":"crossref","first-page":"2491","DOI":"10.1016\/j.carres.2003.08.010","article-title":"Biosynthesis and assembly of group 1 capsular polysaccharides in Escherichia coli and related extracellular polysaccharides in other bacteria","volume":"338","author":"Whitfield","year":"2003","journal-title":"Carbohydr Res"},{"key":"R69","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1046\/j.1365-2958.1999.01276.x","article-title":"Structure, assembly and regulation of expression of capsules in Escherichia coli","volume":"31","author":"Whitfield","year":"1999","journal-title":"Mol Microbiol"},{"key":"R70","doi-asserted-by":"crossref","first-page":"7945","DOI":"10.1073\/pnas.95.14.7945","article-title":"Activity of the yeast MNN1 \u03b1-1,3-mannosyltransferase requires a motif conserved in many other families of glycosyltransferases","volume":"95","author":"Wiggins","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R71","doi-asserted-by":"crossref","first-page":"13543","DOI":"10.1073\/pnas.0705833104","article-title":"Structural basis for the acyl chain selectivity and mechanism of UDP-N-acetylglucosamine acyltransferase","volume":"104","author":"Williams","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R72","doi-asserted-by":"crossref","first-page":"4492","DOI":"10.1128\/JB.00315-09","article-title":"Genome sequencing and comparative analysis of Klebsiella pneumoniae NTUH-K2044, a strain causing liver abscess and meningitis","volume":"191","author":"Wu","year":"2009","journal-title":"J Bacteriol"},{"key":"R73","doi-asserted-by":"crossref","first-page":"986","DOI":"10.3201\/eid1307.070187","article-title":"Virulence characteristics of Klebsiella and clinical manifestations of K. pneumoniae bloodstream infections","volume":"13","author":"Yu","year":"2007","journal-title":"Emerg Infect Dis"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.029017-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T05:24:02Z","timestamp":1605590642000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.029017-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12,1]]},"references-count":73,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2009,12,1]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.029017-0","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,12,1]]}}}