{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T02:38:02Z","timestamp":1789785482157,"version":"4.0.1"},"reference-count":42,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,10,1]]},"abstract":"<jats:p>Lipophilic yeasts of the genus<jats:italic>Malassezia<\/jats:italic>are associated with several skin diseases, such as pityriasis versicolor,<jats:italic>Malassezia<\/jats:italic>folliculitis, seborrhoeic dermatitis and atopic dermatitis, and are also increasingly associated with catheter-related fungaemia. The cell wall components of pathogenic micro-organisms behave as an antigen and\/or ligand of the innate immune response. Live cells of<jats:italic>Malassezia furfur<\/jats:italic>and<jats:italic>Malassezia pachydermatis<\/jats:italic>did not react with an anti-<jats:italic>\u03b1<\/jats:italic>-1,2-mannoside antibody. However, they showed a strong hydrophobicity and reactivity with an anti-<jats:italic>\u03b2<\/jats:italic>-1,3-glucan antibody compared to those of<jats:italic>C. albicans<\/jats:italic>. The cell wall polysaccharides of<jats:italic>M. furfur<\/jats:italic>and<jats:italic>M. pachydermatis<\/jats:italic>were isolated and their structures analysed by<jats:sup>1<\/jats:sup>H and<jats:sup>13<\/jats:sup>C NMR experiments. Both polysaccharides were shown to be<jats:italic>\u03b2<\/jats:italic>-1,6-linked linear galactofuranosyl polymers with a small amount of mannan. The presence of galactomannan on cells of<jats:italic>Malassezia<\/jats:italic>species has not been described previously. The galactomannan did not react with an anti-<jats:italic>Aspergillus fumigatus<\/jats:italic>monoclonal antibody which has specificity for<jats:italic>\u03b2<\/jats:italic>-1,5-linked galactofuranosyl residues. An anti-<jats:italic>M. furfur<\/jats:italic>antibody strongly reacted with the galactomannans of<jats:italic>M. furfur<\/jats:italic>and<jats:italic>M. pachydermatis,<\/jats:italic>but did not react with the galactomannans of<jats:italic>Trichophyton rubrum<\/jats:italic>,<jats:italic>A. fumigatus<\/jats:italic>or<jats:italic>Fonsecaea pedrosoi.<\/jats:italic>The characteristics of the anti-<jats:italic>M. furfur<\/jats:italic>antibody suggest that there is potential for diagnosis of<jats:italic>Malassezia<\/jats:italic>infections by antigen detection.<\/jats:p>","DOI":"10.1099\/mic.0.029967-0","type":"journal-article","created":{"date-parts":[[2009,9,29]],"date-time":"2009-09-29T00:25:45Z","timestamp":1254183945000},"page":"3420-3429","source":"Crossref","is-referenced-by-count":38,"title":["The cell wall galactomannan antigen from Malassezia furfur and Malassezia pachydermatis contains \u03b2-1,6-linked linear galactofuranosyl residues and its detection has diagnostic potential"],"prefix":"10.1099","volume":"155","author":[{"given":"Nobuyuki","family":"Shibata","sequence":"first","affiliation":[{"name":"Department of Infection and Host Defense, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomomi","family":"Saitoh","sequence":"additional","affiliation":[{"name":"Department of Infection and Host Defense, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukari","family":"Tadokoro","sequence":"additional","affiliation":[{"name":"Department of Infection and Host Defense, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshio","family":"Okawa","sequence":"additional","affiliation":[{"name":"Department of Infection and Host Defense, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"8568","DOI":"10.1021\/bi00099a012","article-title":"Complete structure of the cell surface polysaccharide of Streptococcus oralis C104: a 600-MHz NMR study","volume":"30","author":"Abeygunawardana","year":"1991","journal-title":"Biochemistry"},{"key":"R2","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.1099\/00221287-147-7-1839","article-title":"Comparison of cell-wall polysaccharides from Nectria cinnabarina with those from the group of Nectria with Sesquicillium anamorphs","volume":"147","author":"Ahrazem","year":"2001","journal-title":"Microbiology"},{"key":"R3","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1128\/CMR.15.1.21-57.2002","article-title":"Immunology of diseases associated with Malassezia species","volume":"15","author":"Ashbee","year":"2002","journal-title":"Clin Microbiol Rev"},{"key":"R4","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1128\/EC.5.2.238-247.2006","article-title":"MNN5 encodes an iron-regulated alpha-1,2-mannosyltransferase important for protein glycosylation, cell wall integrity, morphogenesis, and virulence in Candida albicans","volume":"5","author":"Bai","year":"2006","journal-title":"Eukaryot Cell"},{"key":"R5","doi-asserted-by":"crossref","first-page":"5589","DOI":"10.1021\/bi00318a032","article-title":"Carbohydrate structures of three novel phosphoinositol-containing sphingolipids from the yeast Histoplasma capsulatum","volume":"23","author":"Barr","year":"1984","journal-title":"Biochemistry"},{"key":"R6","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1074\/jbc.M510360200","article-title":"Outer chain N-glycans are required for cell wall integrity and virulence of Candida albicans","volume":"281","author":"Bates","year":"2006","journal-title":"J Biol Chem"},{"key":"R7","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1155\/2005\/739101","article-title":"Evaluation of relative yeast cell surface hydrophobicity measured by flow cytometry","volume":"13","author":"Colling","year":"2005","journal-title":"Infect Dis Obstet Gynecol"},{"key":"R8","doi-asserted-by":"crossref","first-page":"2504","DOI":"10.1139\/v79-399","article-title":"The conformations of furanosides. 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