{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:55:36Z","timestamp":1792641336888,"version":"4.5.0"},"reference-count":38,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,3,1]]},"abstract":"<jats:p>\n                  <jats:italic>Listeria monocytogenes<\/jats:italic> is a ubiquitous bacterium capable of infecting humans, particularly pregnant women and immunocompromised individuals. Although the intracellular invasion and pathogenesis of listeriosis in mammalian tissues has been well studied, little is known about the ecology of <jats:italic>L. monocytogenes<\/jats:italic>, and in particular the environmental reservoir for this bacterium has not been identified. This study used short-term co-culture at 15, 22 and 37\u2005\u00b0C to examine the interaction of <jats:italic>L. monocytogenes<\/jats:italic> strains with <jats:italic>Acanthamoeba polyphaga<\/jats:italic> ACO12. Survival of <jats:italic>L. monocytogenes<\/jats:italic> cells phagocytosed by monolayers of trophozoites was assessed by culture techniques and microscopy. <jats:italic>A. polyphaga<\/jats:italic> trophozoites eliminated bacterial cells within a few hours post-phagocytosis, irrespective of the incubation temperature used. Wild-type <jats:italic>L. monocytogenes<\/jats:italic> and a phenotypic listeriolysin O mutant were unable to either multiply or survive within trophozoites. By contrast, <jats:italic>Salmonella enterica<\/jats:italic> serovar Typhimurium C5 cells used as controls were able to survive and multiply within <jats:italic>A. polyphaga<\/jats:italic> trophozoites. The data presented indicate that <jats:italic>A. polyphaga<\/jats:italic> ACO12 is unlikely to harbour <jats:italic>L. monocytogenes<\/jats:italic>, or act as an environmental reservoir for this bacterium.<\/jats:p>","DOI":"10.1099\/mic.0.031146-0","type":"journal-article","created":{"date-parts":[[2009,11,6]],"date-time":"2009-11-06T01:41:37Z","timestamp":1257471697000},"page":"809-818","source":"Crossref","is-referenced-by-count":17,"title":["Listeria monocytogenes does not survive ingestion by Acanthamoeba polyphaga"],"prefix":"10.1099","volume":"156","author":[{"given":"Alisha","family":"Akya","sequence":"first","affiliation":[{"name":"School of Molecular and Biomedical Science, The University of Adelaide, Adelaide 5005, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Pointon","sequence":"additional","affiliation":[{"name":"South Australian Research and Development Institute, Glenside, South 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