{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T03:30:55Z","timestamp":1762659055125},"reference-count":29,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,3,1]]},"abstract":"<jats:p>\n                  <jats:italic>Streptomyces ansochromogenes<\/jats:italic> SanG is a pathway-specific regulator that mainly controls the transcription of two transcriptional units involved in nikkomycin biosynthesis. SanG consists of three major functional domains: an N-terminal <jats:italic>Streptomyces<\/jats:italic> antibiotic regulatory protein (SARP) domain, a central ATPase domain, and a C-terminal half homologous to guanylate cyclases belonging to the LuxR family. SanG was expressed in <jats:italic>Escherichia coli<\/jats:italic> as a C-terminally His<jats:sub>6<\/jats:sub>-tagged protein. The purified SanG-His<jats:sub>6<\/jats:sub> was shown to be a dimer in solution by dynamic light scattering. An electrophoretic mobility-shift assay showed that the purified SanG protein could bind to the DNA fragment containing the bidirectional <jats:italic>sanN\u2013sanO<\/jats:italic> promoter region. The SanG-binding sites within the bidirectional <jats:italic>sanN\u2013sanO<\/jats:italic> promoter region were determined by footprinting analysis and identified a consensus-directed repeat sequence 5\u2032-CGGCAAG-3\u2032. SanG showed significant ATPase\/GTPase activity <jats:italic>in vitro<\/jats:italic>, and addition of ATP\/GTP enhanced the affinity of SanG for target DNA, but ATP\/GTP hydrolysis was not essential for SanG binding to the target DNA. However, real-time reverse transcription PCR showed that mutation of the ATPase\/GTPase domain of SanG significantly decreased the transcriptional level of <jats:italic>sanN\u2013I<\/jats:italic> and <jats:italic>sanO\u2013V<\/jats:italic>. These results indicated that the ATPase\/GTPase activity of SanG modulated the transcriptional activation of SanG target genes during nikkomycin biosynthesis.<\/jats:p>","DOI":"10.1099\/mic.0.033605-0","type":"journal-article","created":{"date-parts":[[2009,12,4]],"date-time":"2009-12-04T02:11:10Z","timestamp":1259892670000},"page":"828-837","source":"Crossref","is-referenced-by-count":29,"title":["SanG, a transcriptional activator, controls nikkomycin biosynthesis through binding to the sanN\u2013sanO intergenic region in Streptomyces ansochromogenes"],"prefix":"10.1099","volume":"156","author":[{"given":"Xihong","family":"He","sequence":"first","affiliation":[{"name":"Graduate School of Chinese Academy of Sciences, Beijing100039, China"},{"name":"The Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China"},{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of 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