{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:29:01Z","timestamp":1787164141892,"version":"build-2736575974"},"reference-count":67,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,7,1]]},"abstract":"<jats:p>\n                    Optical maps were generated for 33 uropathogenic\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (UPEC) isolates. For individual genomes, the\n                    <jats:italic>Nco<\/jats:italic>\n                    I restriction fragments aligned into a unique chromosome map for each individual isolate, which was then compared with the\n                    <jats:italic>in silico<\/jats:italic>\n                    restriction maps of all of the sequenced\n                    <jats:italic>E. coli<\/jats:italic>\n                    and\n                    <jats:italic>Shigella<\/jats:italic>\n                    strains. All of the UPEC isolates clustered separately from the\n                    <jats:italic>Shigella<\/jats:italic>\n                    strains as well as the laboratory and enterohaemorrhagic\n                    <jats:italic>E. coli<\/jats:italic>\n                    strains. Moreover, the individual strains appeared to cluster into distinct subgroups based on the dendrogram analyses. Phylogenetic grouping of these 33 strains showed that 32\/33 were the B2 subgroup and 1\/33 was subgroup A. To further characterize the similarities and differences among the 33 isolates, pathogenicity island (PAI), haemolysin and virulence gene comparisons were performed. A strong correlation was observed between individual subgroups and virulence factor genes as well as haemolysis activity. Furthermore, there was considerable conservation of sequenced-strain PAIs in the specific subgroups. Strains with different antibiotic-resistance patterns also appeared to sort into separate subgroups. Thus, the optical maps distinguished the UPEC strains from other\n                    <jats:italic>E. coli<\/jats:italic>\n                    strains and further subdivided the strains into distinct subgroups. This optical mapping procedure holds promise as an alternative way to subgroup all\n                    <jats:italic>E. coli<\/jats:italic>\n                    strains, including those involved in infections outside of the intestinal tract and epidemic strains with distinct patterns of antibiotic resistance.\n                  <\/jats:p>","DOI":"10.1099\/mic.0.033977-0","type":"journal-article","created":{"date-parts":[[2010,4,8]],"date-time":"2010-04-08T20:51:29Z","timestamp":1270759889000},"page":"2124-2135","source":"Crossref","is-referenced-by-count":20,"title":["Use of optical mapping to sort uropathogenic Escherichia coli strains into distinct subgroups"],"prefix":"10.1099","volume":"156","author":[{"given":"William R.","family":"Schwan","sequence":"first","affiliation":[{"name":"University of Wisconsin-La Crosse, La Crosse, WI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Briska","sequence":"additional","affiliation":[{"name":"OpGen Inc., 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