{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T10:05:37Z","timestamp":1790935537607,"version":"4.1.0"},"reference-count":37,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,4,1]]},"abstract":"<jats:p>To study the role of carbohydrates, in particular glucose, glucose 6-phosphate and mannose, as carbon substrates for extra- and intracellular replication of facultative intracellular enteric bacteria, mutants of two enteroinvasive<jats:italic>Escherichia coli<\/jats:italic>(EIEC) strains and a<jats:italic>Salmonella enterica<\/jats:italic>serovar Typhimurium isolate were constructed that were defective in the uptake of glucose and mannose (\u0394<jats:italic>ptsG<\/jats:italic>,<jats:italic>manXYZ<\/jats:italic>), glucose 6-phosphate (\u0394<jats:italic>uhpT<\/jats:italic>) or all three carbohydrates (\u0394<jats:italic>ptsG<\/jats:italic>,<jats:italic>manXYZ<\/jats:italic>,<jats:italic>uhpT<\/jats:italic>). The ability of these mutants to grow in RPMI medium containing the respective carbohydrates and in Caco-2 cells was compared with that of the corresponding wild-type strains. In the three strains, deletions of<jats:italic>ptsG<\/jats:italic>,<jats:italic>manXYZ<\/jats:italic>or<jats:italic>uhpT<\/jats:italic>resulted in considerably different levels of inhibition of growth<jats:italic>in vitro<\/jats:italic>in the presence of glucose, mannose and glucose 6-phosphate, respectively, but hardly reduced their capability for intracellular replication in Caco-2 cells. Even the triple mutants \u0394<jats:italic>ptsG<\/jats:italic>,<jats:italic>manXYZ<\/jats:italic>,<jats:italic>uhpT<\/jats:italic>of the three enterobacterial strains were still able to replicate in Caco-2 cells, albeit at strain-specific lower rates than the corresponding wild-type strains.<\/jats:p>","DOI":"10.1099\/mic.0.034744-0","type":"journal-article","created":{"date-parts":[[2010,1,15]],"date-time":"2010-01-15T01:25:02Z","timestamp":1263518702000},"page":"1176-1187","source":"Crossref","is-referenced-by-count":39,"title":["Glucose and glucose 6-phosphate as carbon sources in extra- and intracellular growth of enteroinvasive Escherichia coli and Salmonella enterica"],"prefix":"10.1099","volume":"156","author":[{"given":"Andreas","family":"G\u00f6tz","sequence":"first","affiliation":[{"name":"Lehrstuhl f\u00fcr Mikrobiologie, Biozentrum der Universit\u00e4t W\u00fcrzburg, D-97074 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Goebel","sequence":"additional","affiliation":[{"name":"Lehrstuhl f\u00fcr Mikrobiologie, Biozentrum der Universit\u00e4t W\u00fcrzburg, D-97074 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"11105","DOI":"10.1073\/pnas.0402424101","article-title":"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors","volume":"101","author":"Bell","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R2","doi-asserted-by":"crossref","first-page":"3235","DOI":"10.1093\/emboj\/19.13.3235","article-title":"Salmonella maintains the integrity of its intracellular vacuole through the action of SifA","volume":"19","author":"Beuz\u00f3n","year":"2000","journal-title":"EMBO J"},{"key":"R3","doi-asserted-by":"crossref","first-page":"2705","DOI":"10.1099\/00221287-148-9-2705","article-title":"Growth and killing of a Salmonella enterica serovar Typhimurium sifA mutant strain in the cytosol of different host cell lines","volume":"148","author":"Beuz\u00f3n","year":"2002","journal-title":"Microbiology"},{"key":"R4","first-page":"153","article-title":"Construction and characterization of new cloning vehicles. 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