{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:40:39Z","timestamp":1791200439131,"version":"4.2.0"},"reference-count":34,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,4,1]]},"abstract":"<jats:p>In this report we examine the PEP-dependent phosphotransferase systems (PTSs) of<jats:italic>Listeria monocytogenes<\/jats:italic>EGD-e, especially those involved in glucose and cellobiose transport. This<jats:italic>L. monocytogenes<\/jats:italic>strain possesses in total 86<jats:italic>pts<\/jats:italic>genes, encoding 29 complete PTSs for the transport of carbohydrates and sugar alcohols, and several single PTS components, possibly supporting transport of these compounds. By a systematic deletion analysis we identified the major PTSs involved in glucose, mannose and cellobiose transport, when<jats:italic>L. monocytogenes<\/jats:italic>grows in a defined minimal medium in the presence of these carbohydrates. Whereas all four PTS permeases belonging to the PTS<jats:sup>Man<\/jats:sup>family may be involved in mannose transport, only two of these (PTS<jats:sup>Man<\/jats:sup>-2 and PTS<jats:sup>Man<\/jats:sup>-3), and in addition at least one (PTS<jats:sup>Glc<\/jats:sup>-1) of the five PTS permeases belonging to the PTS<jats:sup>Glc<\/jats:sup>family, are able to transport glucose, albeit with different efficiencies. Cellobiose is transported mainly by one (PTS<jats:sup>Lac<\/jats:sup>-4) of the six members belonging to the PTS<jats:sup>Lac<\/jats:sup>family. In addition, PTS<jats:sup>Glc<\/jats:sup>-1 appears to be also able to transport cellobiose. The transcription of the operons encoding PTS<jats:sup>Man<\/jats:sup>-2 and PTS<jats:sup>Lac<\/jats:sup>-4 (but not that of the operon for PTS<jats:sup>Man<\/jats:sup>-3) is regulated by LevR-homologous PTS regulation domain (PRD) activators. Whereas the growth rate of the mutant lacking PTS<jats:sup>Man<\/jats:sup>-2, PTS<jats:sup>Man<\/jats:sup>-3 and PTS<jats:sup>Glc<\/jats:sup>-1 is drastically reduced (compared with the wild-type strain) in the presence of glucose, and that of the mutant lacking PTS<jats:sup>Lac<\/jats:sup>-4 and PTS<jats:sup>Glc<\/jats:sup>-1 in the presence of cellobiose, replication of both mutants within epithelial cells or macrophages is as efficient as that of the wild-type strain.<\/jats:p>","DOI":"10.1099\/mic.0.034934-0","type":"journal-article","created":{"date-parts":[[2010,1,8]],"date-time":"2010-01-08T03:23:31Z","timestamp":1262921011000},"page":"1069-1083","source":"Crossref","is-referenced-by-count":102,"title":["The major PEP-phosphotransferase systems (PTSs) for glucose, mannose and cellobiose of Listeria monocytogenes, and their significance for extra- and intracellular 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