{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T15:54:07Z","timestamp":1791129247398,"version":"4.1.0"},"reference-count":51,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,9,1]]},"abstract":"<jats:p>BacA proteins play key roles in the chronic intracellular infections of<jats:italic>Sinorhizobium meliloti<\/jats:italic>,<jats:italic>Brucella abortus<\/jats:italic>and<jats:italic>Mycobacterium tuberculosis<\/jats:italic>within their respective hosts.<jats:italic>S. meliloti<\/jats:italic>,<jats:italic>B. abortus<\/jats:italic>and<jats:italic>M. tuberculosis<\/jats:italic>BacA-deficient mutants have increased resistance to the thiazole-modified peptide bleomycin. BacA has been previously hypothesized, but not experimentally verified, to be involved in bleomycin uptake. In this paper, we show that a BacA-dependent mechanism is the major route of bleomycin internalization in<jats:italic>S. meliloti<\/jats:italic>. We also determined that the<jats:italic>B. abortus<\/jats:italic>and<jats:italic>S. meliloti<\/jats:italic>BacA proteins are functional homologues and that the<jats:italic>B. abortus<\/jats:italic>BacA protein is involved in the uptake of both bleomycin and proline-rich peptides. Our findings also provide evidence that there is a second, BacA-independent minor mechanism for bleomycin internalization in<jats:italic>S. meliloti<\/jats:italic>. We determined that the BacA-dependent and -independent mechanisms of bleomycin uptake are energy-dependent, consistent with both mechanisms of bleomycin uptake involving transport systems.<\/jats:p>","DOI":"10.1099\/mic.0.039909-0","type":"journal-article","created":{"date-parts":[[2010,5,28]],"date-time":"2010-05-28T00:46:08Z","timestamp":1275007568000},"page":"2702-2713","source":"Crossref","is-referenced-by-count":41,"title":["Internalization of a thiazole-modified peptide in Sinorhizobium meliloti occurs by BacA-dependent and -independent mechanisms"],"prefix":"10.1099","volume":"156","author":[{"given":"Silvia","family":"Wehmeier","sequence":"first","affiliation":[{"name":"School of Medicine and Dentistry, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus F. 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