{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:06:51Z","timestamp":1791425211777,"version":"4.3.3"},"reference-count":56,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,9,1]]},"abstract":"<jats:p>\n                    Biofilm formation enhances the survival and persistence of the facultative human pathogen\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    in natural ecosystems and its transmission during seasonal cholera outbreaks. A major component of the\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    biofilm matrix is the\n                    <jats:italic>Vibrio<\/jats:italic>\n                    polysaccharide (VPS), which is essential for development of three-dimensional biofilm structures. The\n                    <jats:italic>vps<\/jats:italic>\n                    genes are clustered in two regions, the\n                    <jats:italic>vps-<\/jats:italic>\n                    I cluster (\n                    <jats:italic>vpsU<\/jats:italic>\n                    ,\n                    <jats:italic>vpsA\u2013K<\/jats:italic>\n                    , VC0916\u201327) and the\n                    <jats:italic>vps-<\/jats:italic>\n                    II cluster (\n                    <jats:italic>vpsL\u2013Q<\/jats:italic>\n                    , VC0934\u201339), separated by an intergenic region containing the\n                    <jats:italic>rbm<\/jats:italic>\n                    gene cluster that encodes biofilm matrix proteins. In-frame deletions of the\n                    <jats:italic>vps<\/jats:italic>\n                    clusters and genes encoding matrix proteins drastically altered biofilm formation phenotypes. To determine which genes within the\n                    <jats:italic>vps<\/jats:italic>\n                    gene clusters are required for biofilm formation and VPS synthesis, we generated in-frame deletion mutants for all the\n                    <jats:italic>vps<\/jats:italic>\n                    genes. Many of these mutants exhibited reduced capacity to produce VPS and biofilms. Infant mouse colonization assays revealed that mutants lacking either\n                    <jats:italic>vps<\/jats:italic>\n                    clusters or\n                    <jats:italic>rbmA<\/jats:italic>\n                    (encoding secreted matrix protein RbmA) exhibited a defect in intestinal colonization compared to the wild-type. Understanding the roles of the various\n                    <jats:italic>vps<\/jats:italic>\n                    gene products will aid in the biochemical characterization of the VPS biosynthetic pathway and elucidate how\n                    <jats:italic>vps<\/jats:italic>\n                    gene products contribute to VPS biosynthesis, biofilm formation and virulence in\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1099\/mic.0.040196-0","type":"journal-article","created":{"date-parts":[[2010,5,13]],"date-time":"2010-05-13T20:35:53Z","timestamp":1273782953000},"page":"2757-2769","source":"Crossref","is-referenced-by-count":265,"title":["Role of Vibrio polysaccharide (vps) genes in VPS production, biofilm formation and Vibrio cholerae pathogenesis"],"prefix":"10.1099","volume":"156","author":[{"given":"Jiunn C. 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