{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T13:54:40Z","timestamp":1686837280976},"reference-count":57,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,4,1]]},"abstract":"<jats:p>The replacement of the bladder with a neobladder made from ileal tissue is the prescribed treatment in some cases of bladder cancer or trauma. Studies have demonstrated that individuals with an ileal neobladder have recurrent colonization by <jats:italic>Escherichia coli<\/jats:italic> and other species that are commonly associated with urinary tract infections; however, pyelonephritis and complicated symptomatic infections with ileal neobladders are relatively rare. This study examines the genomic content of two <jats:italic>E. coli<\/jats:italic> isolates from individuals with neobladders using comparative genomic hybridization (CGH) with a pan-<jats:italic>E. coli<\/jats:italic>\/<jats:italic>Shigella<\/jats:italic> microarray. Comparisons of the neobladder genome hybridization patterns with reference genomes demonstrate that the neobladder isolates are more similar to the commensal, laboratory-adapted <jats:italic>E. coli<\/jats:italic> and a subset of enteroaggregative <jats:italic>E. coli<\/jats:italic> than they are to uropathogenic <jats:italic>E. coli<\/jats:italic> isolates. Genes identified by CGH as exclusively present in the neobladder isolates among the 30 examined isolates were primarily from large enteric isolate plasmids. Isolations identified a large plasmid in each isolate, and sequencing confirmed similarity to previously identified plasmids of enteric species. Screening, via PCR, of more than 100 isolates of <jats:italic>E. coli<\/jats:italic> from environmental, diarrhoeagenic and urinary tract sources did not identify neobladder-specific genes that were widely distributed in these populations. These results taken together demonstrate that the neobladder isolates, while distinct, are genomically more similar to gastrointestinal or commensal <jats:italic>E. coli<\/jats:italic>, suggesting why they can colonize the transplanted intestinal tissue but rarely progress to acute pyelonephritis or more severe disease.<\/jats:p>","DOI":"10.1099\/mic.0.043018-0","type":"journal-article","created":{"date-parts":[[2011,1,21]],"date-time":"2011-01-21T02:51:19Z","timestamp":1295578279000},"page":"1088-1102","source":"Crossref","is-referenced-by-count":8,"title":["Genomic characterization of asymptomatic Escherichia coli isolated from the neobladder"],"prefix":"10.1099","volume":"157","author":[{"given":"Jason W.","family":"Sahl","sequence":"first","affiliation":[{"name":"Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda L.","family":"Lloyd","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School, 1150 West Medical Center Drive, 5641 Medical Science II, Ann Arbor, MI 48109, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia C.","family":"Redman","sequence":"additional","affiliation":[{"name":"Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas A.","family":"Cebula","sequence":"additional","affiliation":[{"name":"Johns Hopkins University, Department of Biology, 3400 North Charles Street, Baltimore, MD 21218, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David P.","family":"Wood","sequence":"additional","affiliation":[{"name":"University of Michigan Medical School, Department of Urology, 1500 East Medical Center Drive, Ann Arbor, MI 48109, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harry L. 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