{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T18:44:30Z","timestamp":1772045070777,"version":"3.50.1"},"reference-count":51,"publisher":"Microbiology Society","issue":"5","funder":[{"DOI":"10.13039\/501100003134","name":"Fonds pour la Formation \u00e0 la Recherche dans l\u2019Industrie et dans l\u2019Agriculture","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003134","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Actions de Recherches Concert\u00e9es fellowship","award":["04\/09-325"],"award-info":[{"award-number":["04\/09-325"]}]},{"name":"Actions de Recherches Concert\u00e9es fellowship","award":["08\/13-015"],"award-info":[{"award-number":["08\/13-015"]}]},{"name":"Fonds de la Recherche Fondamentale Collective","award":["2.4521.04"],"award-info":[{"award-number":["2.4521.04"]}]},{"name":"Fonds de la Recherche Fondamentale Collective","award":["2.4521.08"],"award-info":[{"award-number":["2.4521.08"]}]},{"name":"Commission of the European Communities","award":["QLK2-CT-1999-00014"],"award-info":[{"award-number":["QLK2-CT-1999-00014"]}]},{"name":"Fonds National de la Recherche Scientifique"},{"name":"Fonds Adrien Bauchau"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,5,1]]},"abstract":"<jats:p>It was recently demonstrated that the pathogen<jats:italic>Brucella melitensis<\/jats:italic>produces a polar sheathed flagellum under the control of the master regulator FtcR. However, the regulatory mechanism controlling the flagellar assembly remains unknown. In this work, we investigate the flagellar hierarchy of<jats:italic>B. melitensis<\/jats:italic>as well as the flagellin FliC regulation. We show that a mutation in<jats:italic>fliF<\/jats:italic>or<jats:italic>flgE<\/jats:italic>(coding for the basal body structure and the hook, respectively) does not affect FliC synthesis, suggesting that production of FliC does not depend on the flagellar assembly. We demonstrate that FlbT is a FliC activator since inactivation of<jats:italic>flbT<\/jats:italic>causes a decrease in<jats:italic>fliC<\/jats:italic>expression by using a<jats:italic>fliC<\/jats:italic>\u2013<jats:italic>lacZ<\/jats:italic>translational reporter construct. Moreover, the quantitative real-time PCR and Western blot analysis show a marked decrease in<jats:italic>fliC<\/jats:italic>mRNA and FliC protein level, respectively. Conversely, the<jats:italic>B. melitensis<\/jats:italic>wild-type strain overexpressing<jats:italic>flaF<\/jats:italic>fails to produce FliC, suggesting an opposite function. Interestingly, the expression of the<jats:italic>flbT<\/jats:italic>gene in an<jats:italic>ftcR<\/jats:italic>or an<jats:italic>flbT<\/jats:italic>mutant restores FliC production, demonstrating that FlbT plays a regulatory checkpoint role in FliC synthesis. This mechanism could be conserved in the<jats:italic>Rhizobiales<\/jats:italic>since complementation of an<jats:italic>flbT<\/jats:italic>or an<jats:italic>ftcR<\/jats:italic>mutant with<jats:italic>flbT<\/jats:italic>from<jats:italic>Sinorhizobium meliloti<\/jats:italic>restores FliC synthesis.<\/jats:p>","DOI":"10.1099\/mic.0.044867-0","type":"journal-article","created":{"date-parts":[[2011,1,28]],"date-time":"2011-01-28T02:46:30Z","timestamp":1296182790000},"page":"1253-1262","source":"Crossref","is-referenced-by-count":23,"title":["Role of FlbT in flagellin production in Brucella melitensis"],"prefix":"10.1099","volume":"157","author":[{"given":"Jonathan","family":"Ferooz","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie Mol\u00e9culaire (URBM), Facult\u00e9s Universitaires Notre-Dame de la Paix Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien","family":"Lemaire","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie Mol\u00e9culaire (URBM), Facult\u00e9s Universitaires Notre-Dame de la Paix Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Jacques","family":"Letesson","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie Mol\u00e9culaire (URBM), Facult\u00e9s Universitaires Notre-Dame de la Paix Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, 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