{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:16:35Z","timestamp":1789996595018,"version":"4.0.1"},"reference-count":26,"publisher":"Microbiology Society","issue":"5","funder":[{"name":"Fonds pour la Formation \u00e0 la Recherche dans l\u2019Industrie et dans lAgriculture"},{"name":"Fonds National de la Recherche Scientifique"},{"name":"Fonds Adrien Bauchau"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,5,1]]},"abstract":"<jats:p>The genome of <jats:italic>Brucella melitensis<\/jats:italic> contains genes coding for the sigma factors RpoD, RpoN, RpoH1, RpoH2, RpoE1 and RpoE2. Previously published data show that <jats:italic>B. melitensis<\/jats:italic> is flagellated and that an <jats:italic>rpoE1<\/jats:italic> mutant overexpresses the flagellar protein FlgE. In this study, we demonstrate that mutation of <jats:italic>rpoE1<\/jats:italic> causes an overexpression of the flagellar genes <jats:italic>fliF<\/jats:italic>, <jats:italic>flgE<\/jats:italic>, <jats:italic>fliC<\/jats:italic>, <jats:italic>flaF<\/jats:italic> and <jats:italic>flbT<\/jats:italic>, correlating with the production of a longer filament and thereby demonstrating that RpoE1 acts as a flagellar repressor. Moreover, mutation of <jats:italic>rpoE1<\/jats:italic> increases the promoter activity of the flagellar master regulator <jats:italic>ftcR<\/jats:italic>, suggesting that RpoE1 acts upstream of <jats:italic>ftcR.<\/jats:italic> Together, these data show that RpoE1 represses the flagellar synthesis and filament length in <jats:italic>B. melitensis<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/mic.0.044875-0","type":"journal-article","created":{"date-parts":[[2011,1,28]],"date-time":"2011-01-28T02:46:30Z","timestamp":1296182790000},"page":"1263-1268","source":"Crossref","is-referenced-by-count":22,"title":["RpoE1, an extracytoplasmic function sigma factor, is a repressor of the flagellar system in Brucella melitensis"],"prefix":"10.1099","volume":"157","author":[{"given":"Jonathan","family":"Ferooz","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie Mol\u00e9culaire (URBM), Facult\u00e9s Universitaires Notre-Dame de la Paix Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Lemaire","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie Mol\u00e9culaire (URBM), Facult\u00e9s Universitaires Notre-Dame de la Paix Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Delory","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie Mol\u00e9culaire (URBM), Facult\u00e9s Universitaires Notre-Dame de la Paix Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"De Bolle","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie Mol\u00e9culaire (URBM), Facult\u00e9s Universitaires Notre-Dame de la Paix Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Letesson","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie Mol\u00e9culaire (URBM), Facult\u00e9s Universitaires Notre-Dame de la Paix Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.06005.x"},{"key":"r2","doi-asserted-by":"crossref","first-page":"5574","DOI":"10.1128\/JB.179.17.5574-5581.1997","article-title":"A transcriptional activator, FleQ, regulates mucin adhesion and flagellar gene expression in Pseudomonas aeruginosa in a cascade manner","volume":"179","author":"Arora","year":"1997","journal-title":"J Bacteriol"},{"key":"r3","volume-title":"Current Protocols in Molecular Biology","author":"Ausubel","year":"1991"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.14.4613-4619.2004"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00644-06"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-3-333"},{"key":"r7","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1099\/mic.0.044867-0","article-title":"Role of FlbT in flagellin production in Brucella melitensis","volume":"157","author":"Ferooz","year":"2011","journal-title":"Microbiology"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0810291106"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00502.x"},{"key":"r10","doi-asserted-by":"crossref","first-page":"7401","DOI":"10.1128\/JB.181.23.7401-7404.1999","article-title":"Negative control of flagellum synthesis in Pseudomonas aeruginosa is modulated by the alternative sigma factor AlgT (AlgU)","volume":"181","author":"Garrett","year":"1999","journal-title":"J Bacteriol"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06769.x"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00712-06"},{"key":"r13","doi-asserted-by":"crossref","first-page":"7149","DOI":"10.1128\/JB.181.23.7149-7153.1999","article-title":"The bacterial flagellum: reversible rotary propellor and type III export apparatus","volume":"181","author":"Macnab","year":"1999","journal-title":"J Bacteriol"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01626-08"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1039\/b808065h"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040002"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00175-07"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1183-784"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2164(08)01008-3"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.23.7955-7962.2005"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00636-06"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn304"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.97"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1021\/pr100068p"},{"key":"r25","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07079.x"},{"key":"r26","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.09.002"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.044875-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T11:51:37Z","timestamp":1605786697000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.044875-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,5,1]]},"references-count":26,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2011,5,1]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.044875-0","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,5,1]]}}}