{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T18:45:12Z","timestamp":1790966712590,"version":"4.1.0"},"reference-count":41,"publisher":"Microbiology Society","issue":"8","funder":[{"DOI":"10.13039\/501100006488","name":"Institut National de la Recherche Agronomique","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006488","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,8,1]]},"abstract":"<jats:p>\n                  <jats:italic>Streptococcus agalactiae<\/jats:italic> is the leading cause of bacterial sepsis and meningitis in neonates and is also the causative agent of several serious infections in immunocompromised adults. <jats:italic>S. agalactiae<\/jats:italic> encounters multiple niches during an infection, suggesting that regulatory mechanisms control the expression of specific virulence factors in this bacterium. The present study describes the functional characterization of a gene from <jats:italic>S. agalactiae<\/jats:italic>, designated <jats:italic>rga<\/jats:italic>, which encodes a protein with significant similarity to members of the RofA-like protein (RALP) family of transcriptional regulators. After deletion of the <jats:italic>rga<\/jats:italic> gene in the genome of <jats:italic>S. agalactiae<\/jats:italic>, the mutant strain exhibited significantly reduced expression of the genes <jats:italic>srr-1<\/jats:italic> and <jats:italic>pilA,<\/jats:italic> which encode a serine-rich repeat surface glycoprotein and a pilus protein, respectively, and moderately increased expression of the <jats:italic>fbsA<\/jats:italic> gene, which encodes a fibrinogen-binding protein. Electrophoretic mobility shift assays demonstrated specific DNA binding of purified Rga to the promoter regions of <jats:italic>pilA<\/jats:italic> and <jats:italic>fbsA<\/jats:italic>, suggesting that Rga directly controls <jats:italic>pilA<\/jats:italic> and <jats:italic>fbsA<\/jats:italic>. Adherence assays revealed significantly reduced binding of the \u0394<jats:italic>rga<\/jats:italic> mutant to epithelial HEp-2 cells and to immobilized human keratin 4, respectively. In contrast, the adherence of the \u0394<jats:italic>rga<\/jats:italic> mutant to A549 cells and its binding to human fibrinogen was significantly increased. Immunoblot and immunoelectron microscopy revealed that the quantity of pilus structures was significantly reduced in the \u0394<jats:italic>rga<\/jats:italic> mutant compared with the parental strain. The wild-type phenotype could be restored by plasmid-mediated expression of <jats:italic>rga<\/jats:italic>, demonstrating that the mutant phenotypes resulted from a loss of Rga function.<\/jats:p>","DOI":"10.1099\/mic.0.044933-0","type":"journal-article","created":{"date-parts":[[2011,2,18]],"date-time":"2011-02-18T03:22:47Z","timestamp":1297999367000},"page":"2319-2327","source":"Crossref","is-referenced-by-count":20,"title":["Rga is a regulator of adherence and pilus formation in Streptococcus agalactiae"],"prefix":"10.1099","volume":"157","author":[{"given":"Ulrike","family":"Samen","sequence":"first","affiliation":[{"name":"Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beate","family":"Heinz","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heike","family":"Boisvert","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard J.","family":"Eikmanns","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter J.","family":"Reinscheid","sequence":"additional","affiliation":[{"name":"University of Applied Sciences Bonn-Rhein-Sieg, 53359 Rheinbach, Germany"},{"name":"Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Borges","sequence":"additional","affiliation":[{"name":"Laboratoire d'Ing\u00e9nierie des Biomol\u00e9cules, Nancy Universit\u00e9, 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