{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T02:00:47Z","timestamp":1783130447587,"version":"3.54.6"},"reference-count":37,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,3,1]]},"abstract":"<jats:p>The alkaliphilic bacterium<jats:italic>Pseudomonas pseudoalcaligenes<\/jats:italic>CECT5344 is able to grow with cyanide as the sole nitrogen source. Membrane fractions from cells grown under cyanotrophic conditions catalysed the production of oxaloacetate from<jats:sc>l<\/jats:sc>-malate. Several enzymic activities of the tricarboxylic acid and glyoxylate cycles in association with the cyanide-insensitive respiratory pathway seem to be responsible for the oxaloacetate formation<jats:italic>in vivo<\/jats:italic>. Thus, in cyanide-grown cells, citrate synthase and isocitrate lyase activities were significantly higher than those observed with other nitrogen sources. Malate dehydrogenase activity was undetectable, but a malate\u200a:\u200aquinone oxidoreductase activity coupled to the cyanide-insensitive alternative oxidase was found in membrane fractions from cyanide-grown cells. Therefore, oxaloacetate production was linked to the cyanide-insensitive respiration in<jats:italic>P. pseudoalcaligenes<\/jats:italic>CECT5344. Cyanide and oxaloacetate reacted chemically inside the cells to produce a cyanohydrin (2-hydroxynitrile), which was further converted to ammonium. In addition to cyanide, strain CECT5344 was able to grow with several cyano derivatives, such as 2- and 3-hydroxynitriles. The specific system required for uptake and metabolization of cyanohydrins was induced by cyanide and by 2-hydroxynitriles, such as the cyanohydrins of oxaloacetate and 2-oxoglutarate.<\/jats:p>","DOI":"10.1099\/mic.0.045286-0","type":"journal-article","created":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T04:50:46Z","timestamp":1293079846000},"page":"739-746","source":"Crossref","is-referenced-by-count":30,"title":["Cyanide degradation by Pseudomonas pseudoalcaligenes CECT5344 involves a malate\u200a:\u200aquinone oxidoreductase and an associated cyanide-insensitive electron transfer chain"],"prefix":"10.1099","volume":"157","author":[{"given":"Victor M.","family":"Luque-Almagro","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Campus de Rabanales, Edificio Severo Ochoa, 1a Planta, Universidad de C\u00f3rdoba, C\u00f3rdoba, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faustino","family":"Merch\u00e1n","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Gen\u00e9tica, Facultad de Veterinaria, Universidad de Extremadura, C\u00e1ceres, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Blasco","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Gen\u00e9tica, Facultad de Veterinaria, Universidad de Extremadura, C\u00e1ceres, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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