{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:59:25Z","timestamp":1791284365028,"version":"4.1.0"},"reference-count":99,"publisher":"Microbiology Society","issue":"7","funder":[{"name":"Cosmetic Valley"},{"name":"Europe","award":["FEDER no. 31970"],"award-info":[{"award-number":["FEDER no. 31970"]}]},{"name":"Conseil G\u00e9n\u00e9ral de l'Eure"},{"name":"Communaut\u00e9 d'Agglom\u00e9ration d'Evreux"},{"name":"Haute-Normandie"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,7,1]]},"abstract":"<jats:p><jats:italic>Pseudomonas aeruginosa<\/jats:italic>coordinates its virulence expression and establishment in the host in response to modification of its environment. During the infectious process, bacteria are exposed to and can detect eukaryotic products including hormones. It has been shown that<jats:italic>P. aeruginosa<\/jats:italic>is sensitive to natriuretic peptides, a family of eukaryotic hormones, through a cyclic nucleotide-dependent sensor system that modulates its cytotoxicity. We observed that pre-treatment of<jats:italic>P. aeruginosa<\/jats:italic>PAO1 with C-type natriuretic peptide (CNP) increases the capacity of the bacteria to kill<jats:italic>Caenorhabditis elegans<\/jats:italic>through diffusive toxin production. In contrast, brain natriuretic peptide (BNP) did not affect the capacity of the bacteria to kill<jats:italic>C. elegans<\/jats:italic>. The bacterial production of hydrogen cyanide (HCN) was enhanced by both BNP and CNP whereas the production of phenazine pyocyanin was strongly inhibited by CNP. The amount of 2-heptyl-4-quinolone (HHQ), a precursor to 2-heptyl-3-hydroxyl-4-quinolone (<jats:italic>Pseudomonas<\/jats:italic>quinolone signal; PQS), decreased after CNP treatment. The quantity of 2-nonyl-4-quinolone (HNQ), another quinolone which is synthesized from HHQ, was also reduced after CNP treatment. Conversely, both BNP and CNP significantly enhanced bacterial production of acylhomoserine lactone (AHL) [e.g. 3-oxo-dodecanoyl-homoserine lactone (3OC12-HSL) and butanoylhomoserine lactone (C4-HSL)]. These results correlate with an induction of<jats:italic>lasI<\/jats:italic>transcription 1 h after bacterial exposure to BNP or CNP. Concurrently, pre-treatment of<jats:italic>P. aeruginosa<\/jats:italic>PAO1 with either BNP or CNP enhanced PAO1 exotoxin A production, via a higher<jats:italic>toxA<\/jats:italic>mRNA level. At the same time, CNP led to elevated amounts of<jats:italic>algC<\/jats:italic>mRNA, indicating that<jats:italic>algC<\/jats:italic>is involved in<jats:italic>C. elegans<\/jats:italic>killing. Finally, we observed that in PAO1, Vfr protein is essential to the pro-virulent effect of CNP whereas the regulator PtxR supports only a part of the CNP pro-virulent activity. Taken together, these data reinforce the hypothesis that during infection natriuretic peptides, particularly CNP, could enhance the virulence of PAO1. This activity is relayed by Vfr and PtxR activation, and a general diagram of the virulence activation cascade involving AHL, HCN and exotoxin A is proposed.<\/jats:p>","DOI":"10.1099\/mic.0.046755-0","type":"journal-article","created":{"date-parts":[[2011,4,22]],"date-time":"2011-04-22T00:56:17Z","timestamp":1303433777000},"page":"1929-1944","source":"Crossref","is-referenced-by-count":62,"title":["C-type natriuretic peptide modulates quorum sensing molecule and toxin production in Pseudomonas aeruginosa"],"prefix":"10.1099","volume":"157","author":[{"given":"Anne-Sophie","family":"Blier","sequence":"first","affiliation":[{"name":"Laboratory of Cold Microbiology \u2013 Signals and Micro-environment EA 4312, University of Rouen, 55 Rue Saint Germain, 27000 Evreux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilfried","family":"Veron","sequence":"additional","affiliation":[{"name":"Laboratory of Cold Microbiology \u2013 Signals and Micro-environment EA 4312, University of Rouen, 55 Rue Saint Germain, 27000 Evreux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexis","family":"Bazire","sequence":"additional","affiliation":[{"name":"Laboratoire de Biotechnologie et Chimie Marines, Universit\u00e9 de Bretagne-Sud B.P. 92116, 56321 Lorient Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elo\u00efse","family":"Gerault","sequence":"additional","affiliation":[{"name":"Laboratory of Cold Microbiology \u2013 Signals and Micro-environment EA 4312, University of Rouen, 55 Rue Saint Germain, 27000 Evreux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laure","family":"Taupin","sequence":"additional","affiliation":[{"name":"Laboratoire de Biotechnologie et Chimie Marines, Universit\u00e9 de Bretagne-Sud B.P. 92116, 56321 Lorient Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Vieillard","sequence":"additional","affiliation":[{"name":"SIMA, UMR 6014 COBRA, University of Rouen, 27000 Evreux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Rehel","sequence":"additional","affiliation":[{"name":"Laboratoire de Biotechnologie et Chimie Marines, Universit\u00e9 de Bretagne-Sud B.P. 92116, 56321 Lorient Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Dufour","sequence":"additional","affiliation":[{"name":"Laboratoire de Biotechnologie et Chimie Marines, Universit\u00e9 de Bretagne-Sud B.P. 92116, 56321 Lorient Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franck","family":"Le Derf","sequence":"additional","affiliation":[{"name":"SIMA, UMR 6014 COBRA, University of Rouen, 27000 Evreux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Orange","sequence":"additional","affiliation":[{"name":"Laboratory of Cold Microbiology \u2013 Signals and Micro-environment EA 4312, University of Rouen, 55 Rue Saint Germain, 27000 Evreux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Hulen","sequence":"additional","affiliation":[{"name":"Laboratory of Cold Microbiology \u2013 Signals and Micro-environment EA 4312, University of Rouen, 55 Rue Saint Germain, 27000 Evreux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc G. 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