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The structural subunit of<jats:italic>E. coli<\/jats:italic>O157\u200a:\u200aH7 flagella is flagellin, which is both the antigenic determinant of the H7 serotype, an important factor in colonization, and an immunomodulatory protein that has been determined to be a major pro-inflammatory component through the instigation of host cell signalling pathways. Flagellin has highly conserved N- and C-terminal regions that are recognized by the host cell pattern recognition receptor Toll-like receptor (TLR) 5. Activation of this receptor triggers cell signalling cascades, which are known to activate host cell kinases and transcription factors that respond with the production of inflammatory mediators such as the chemokine interleukin-8 (IL-8), although the exact components of this pathway are not yet fully characterized. We demonstrate that<jats:italic>E. coli<\/jats:italic>O157\u200a:\u200aH7-derived flagellin induces rapid phosphorylation of the epidermal growth factor receptor (EGFR), as an early event in intestinal epithelial cell signalling, and that this is required for the release of the pro-inflammatory cytokine IL-8.<\/jats:p>","DOI":"10.1099\/mic.0.047670-0","type":"journal-article","created":{"date-parts":[[2011,5,6]],"date-time":"2011-05-06T04:05:18Z","timestamp":1304654718000},"page":"2339-2347","source":"Crossref","is-referenced-by-count":10,"title":["Phosphorylation of the epidermal growth factor receptor (EGFR) is essential for interleukin-8 release from intestinal epithelial cells in response to challenge with Escherichia coli O157\u200a:\u200aH7 flagellin"],"prefix":"10.1099","volume":"157","author":[{"given":"Douglas J.","family":"Fraser-Pitt","sequence":"first","affiliation":[{"name":"Biomedical Sciences and Microbiology Group, School of Life, Sport and Social Sciences, Faculty of Health, Life and Social Sciences, Sighthill Campus, Edinburgh Napier University, Edinburgh EH11 4BN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Cameron","sequence":"additional","affiliation":[{"name":"Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom N.","family":"McNeilly","sequence":"additional","affiliation":[{"name":"Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Boyd","sequence":"additional","affiliation":[{"name":"Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin D. 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