{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T01:55:37Z","timestamp":1770688537055,"version":"3.49.0"},"reference-count":30,"publisher":"Microbiology Society","issue":"7","funder":[{"name":"Strategic Medical Research Center of the Ministry of Education, Culture, Sports, Science and Technology, Japan"},{"name":"Grants-in-Aid for Scientific Research","award":["20592463"],"award-info":[{"award-number":["20592463"]}]},{"name":"Grants-in-Aid for Scientific Research","award":["20592181"],"award-info":[{"award-number":["20592181"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,7,1]]},"abstract":"<jats:p>A third enzyme that produces hydrogen sulfide from <jats:sc>l<\/jats:sc>-cysteine was identified in <jats:italic>Fusobacterium nucleatum<\/jats:italic> subsp. <jats:italic>nucleatum<\/jats:italic>. The <jats:italic>fn1055<\/jats:italic> gene was cloned from a cosmid library constructed with genomic DNA of <jats:italic>F. nucleatum<\/jats:italic> ATCC 25586. Despite the database annotation that the product of <jats:italic>fn1055<\/jats:italic> is a cysteine synthase, reverse-phase HPLC revealed that no <jats:sc>l<\/jats:sc>-cysteine was produced <jats:italic>in vitro<\/jats:italic> by the purified Fn1055 protein; however, the enzyme did produce <jats:sc>l<\/jats:sc>-serine. In addition, a cysteine auxotroph, <jats:italic>Escherichia coli<\/jats:italic> NK3, transformed with a plasmid containing the <jats:italic>fn1055<\/jats:italic> gene did not grow without cysteine, which further suggests that Fn1055 does not function as a cysteine synthase. The Michaelis\u2013Menten kinetics (<jats:italic>K<\/jats:italic>\n                  <jats:sub>m<\/jats:sub>\u200a=\u200a0.09\u00b10.001 mM and <jats:italic>k<\/jats:italic>\n                  <jats:sub>cat<\/jats:sub>\u200a=\u200a5.43\u00b10.64 s<jats:sup>\u22121<\/jats:sup>) of the purified enzyme showed that the capacity of Fn1055 to produce hydrogen sulfide was between that of two other enzymes, Fn0625 and Fn1220. Incubation of Fn1055 with <jats:sc>l<\/jats:sc>-cysteine resulted in the production of hydrogen sulfide, but not of pyruvate, ammonia or lanthionine, which are all byproducts produced in addition to hydrogen sulfide when Fn0625 or Fn1220 is incubated with <jats:sc>l<\/jats:sc>-cysteine. Instead, Fn1055 produced <jats:sc>l<\/jats:sc>-serine in its reaction with <jats:sc>l<\/jats:sc>-cysteine. Fn1055 produces hydrogen sulfide from <jats:sc>l<\/jats:sc>-cysteine by a mechanism that is different from that of Fn0625 or Fn1220.<\/jats:p>","DOI":"10.1099\/mic.0.048934-0","type":"journal-article","created":{"date-parts":[[2011,4,15]],"date-time":"2011-04-15T00:52:30Z","timestamp":1302828750000},"page":"2164-2171","source":"Crossref","is-referenced-by-count":21,"title":["Identification and enzymic analysis of a novel protein associated with production of hydrogen sulfide and l-serine from l-cysteine in Fusobacterium nucleatum subsp. nucleatum ATCC 25586"],"prefix":"10.1099","volume":"157","author":[{"given":"Yasuo","family":"Yoshida","sequence":"first","affiliation":[{"name":"Department of Microbiology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyosuke","family":"Suwabe","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Oral Rehabilitation, School of Dentistry, Iwate Medical University, Morioka, Japan"},{"name":"Department of Microbiology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiji","family":"Nagano","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuichiro","family":"Kezuka","sequence":"additional","affiliation":[{"name":"Department of Structural Biology, School of Pharmacy, Iwate Medical University, Yahaba, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirohisa","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Pathogenesis and Control of Oral Disease, School of Dentistry, Iwate Medical University, Morioka, 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