{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:33:57Z","timestamp":1790026437936,"version":"4.0.1"},"reference-count":41,"publisher":"Microbiology Society","issue":"8","funder":[{"DOI":"10.13039\/100000774","name":"Defense Threat Reduction Agency","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000774","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Department of Defense Chemical and Biological Defense Program"},{"name":"Transformational Medical Technologies Program","award":["W911NF-08-C-0023"],"award-info":[{"award-number":["W911NF-08-C-0023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,8,1]]},"abstract":"<jats:p>\n                  <jats:italic>Burkholderia pseudomallei<\/jats:italic> is an intracellular pathogen and the causative agent of melioidosis, a life-threatening disease of humans. Within host cells, superoxide is an important mediator of pathogen killing. In this study, we have identified the <jats:italic>B. pseudomallei<\/jats:italic> K96243 <jats:italic>sodC<\/jats:italic> gene, shown that it has superoxide dismutase activity, and constructed an allelic deletion mutant of this gene. Compared with the wild-type, the mutant was more sensitive to killing by extracellular superoxide, but not to superoxide generated intracellularly. The <jats:italic>sodC<\/jats:italic> mutant showed a markedly decreased survival in J774A.1 mouse macrophages, and reduced numbers of bacteria were recovered from human polymorphonuclear neutrophils (PMNs) when compared with the wild-type. The numbers of wild-type or mutant bacteria recovered from human diabetic neutrophils were significantly lower than from normal human neutrophils. The <jats:italic>sodC<\/jats:italic> mutant was attenuated in BALB\/c mice. Our results indicate that SodC plays a key role in the virulence of <jats:italic>B. pseudomallei<\/jats:italic>, but that diabetics are not more susceptible to infection because of a reduced ability of PMNs to kill by superoxide.<\/jats:p>","DOI":"10.1099\/mic.0.050823-0","type":"journal-article","created":{"date-parts":[[2011,6,10]],"date-time":"2011-06-10T08:15:07Z","timestamp":1307693707000},"page":"2392-2400","source":"Crossref","is-referenced-by-count":53,"title":["Superoxide dismutase C is required for intracellular survival and virulence of Burkholderia pseudomallei"],"prefix":"10.1099","volume":"157","author":[{"given":"Muthita","family":"Vanaporn","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Mahidol University, Rajvithi Road, Bangkok 10400, Thailand"},{"name":"College of Life and Environmental Sciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, 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