{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:30:54Z","timestamp":1791405054739,"version":"4.3.3"},"reference-count":38,"publisher":"Microbiology Society","issue":"11","funder":[{"name":"Kansas State University"},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["P20RR16475"],"award-info":[{"award-number":["P20RR16475"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Kansas Technology Enterprise Corporation"},{"name":"National Science Foundation","award":["EPS 0236913"],"award-info":[{"award-number":["EPS 0236913"]}]},{"name":"National Science Foundation","award":["MCB 0455318"],"award-info":[{"award-number":["MCB 0455318"]}]},{"name":"National Science Foundation","award":["0920663"],"award-info":[{"award-number":["0920663"]}]},{"name":"National Science Foundation","award":["DBI 0521587"],"award-info":[{"award-number":["DBI 0521587"]}]},{"name":"NIH\/NIAID","award":["R21AI074077-01A2"],"award-info":[{"award-number":["R21AI074077-01A2"]}]},{"name":"NIH\/NIDCR","award":["R01 DE017486-01A1"],"award-info":[{"award-number":["R01 DE017486-01A1"]}]},{"name":"NIH\/NIDCR","award":["R01DE 13932-4"],"award-info":[{"award-number":["R01DE 13932-4"]}]},{"name":"NIH\/NIAID","award":["RO1 AI035097"],"award-info":[{"award-number":["RO1 AI035097"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,11,1]]},"abstract":"<jats:p>\n                  <jats:italic>Candida albicans<\/jats:italic>-associated bloodstream infections are linked to the ability of this yeast to form biofilms. In this study, we used lipidomics to compare the lipid profiles of <jats:italic>C. albicans<\/jats:italic> biofilms and planktonic cells, in early and mature developmental phases. Our results showed that significant differences exist in lipid composition in both developmental phases. Biofilms contained higher levels of phospholipid and sphingolipids than planktonic cells (nmol per g biomass, <jats:italic>P<\/jats:italic>&lt;0.05 for all comparisons). In the early phase, levels of lipid in most classes were significantly higher in biofilms compared to planktonic cells (<jats:italic>P<\/jats:italic>\u22640.05). The ratio of phosphatidylcholine to phosphatidylethanolamine was lower in biofilms compared to planktonic cells in both early (1.17 vs 2.52, <jats:italic>P<\/jats:italic>\u22640.001) and late (2.34 vs 3.81, <jats:italic>P<\/jats:italic>\u22640.001) developmental phases. The unsaturation index of phospholipids decreased with time, with this effect being particularly strong for biofilms. Inhibition of the biosynthetic pathway for sphingolipid [mannosyl diinositolphosphoryl ceramide, M(IP)<jats:sub>2<\/jats:sub>C] by myriocin or aureobasidin A, and disruption of the gene encoding inositolphosphotransferase (Ipt1p), abrogated the ability of <jats:italic>C. albicans<\/jats:italic> to form biofilms. The differences in lipid profiles between biofilms and planktonic <jats:italic>Candida<\/jats:italic> cells may have important implications for the biology and antifungal resistance of biofilms.<\/jats:p>","DOI":"10.1099\/mic.0.051086-0","type":"journal-article","created":{"date-parts":[[2011,9,9]],"date-time":"2011-09-09T13:21:36Z","timestamp":1315574496000},"page":"3232-3242","source":"Crossref","is-referenced-by-count":119,"title":["Lipidomics of Candida albicans biofilms reveals phase-dependent production of phospholipid molecular classes and role for lipid rafts in biofilm formation"],"prefix":"10.1099","volume":"157","author":[{"given":"Ali Abdul","family":"Lattif","sequence":"first","affiliation":[{"name":"Center for Medical Mycology, University Hospitals Case Medical Center, and Department of Dermatology, Case Western Reserve University, 11100 Euclid Ave, Cleveland, OH 44106-502, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pranab K.","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Center for Medical Mycology, University Hospitals Case Medical Center, and Department of Dermatology, Case Western Reserve University, 11100 Euclid Ave, Cleveland, OH 44106-502, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyotsna","family":"Chandra","sequence":"additional","affiliation":[{"name":"Center for Medical Mycology, University Hospitals Case Medical Center, and Department of Dermatology, Case Western Reserve University, 11100 Euclid Ave, Cleveland, OH 44106-502, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary R.","family":"Roth","sequence":"additional","affiliation":[{"name":"Kansas Lipidomics Research Center, Division of Biology, Kansas State University, Manhattan, KS 66506-4901, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Welti","sequence":"additional","affiliation":[{"name":"Kansas Lipidomics Research Center, Division of Biology, Kansas State University, Manhattan, KS 66506-4901, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud","family":"Rouabhia","sequence":"additional","affiliation":[{"name":"Groupe de Recherche en \u00c9cologie Buccale, Facult\u00e9 de M\u00e9decine Dentaire, Universit\u00e9 Laval, Qu\u00e9bec City, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud A.","family":"Ghannoum","sequence":"additional","affiliation":[{"name":"Center for Medical Mycology, University Hospitals Case Medical Center, and Department of Dermatology, Case Western Reserve University, 11100 Euclid Ave, Cleveland, OH 44106-502, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006852207366"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00633-08"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1993.tb06556.x"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.6.2339"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06628.x"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01841-06"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.192"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.07018.x"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2006.06.005"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.47.29620"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1007\/BF02927612"},{"key":"r12","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1128\/IAI.54.1.189-193.1986","article-title":"Experimental evidence for the role of lipids in adherence of Candida spp. to human buccal epithelial cells","volume":"54","author":"Ghannoum","year":"1986","journal-title":"Infect Immun"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1080\/02681219080000151"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M101568200"},{"key":"r15","first-page":"2421","article-title":"The lipid composition of azole-sensitive and azole-resistant strains of Candida albicans","volume":"132","author":"Hitchcock","year":"1986","journal-title":"J Gen Microbiol"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1080\/02681218780000041"},{"key":"r17","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1007\/978-3-642-79837-5_4","article-title":"Chromatographic analysis of lipids","volume-title":"Manual on Membrane Lipids","author":"Ibrahim","year":"1996"},{"key":"r18","first-page":"1215","article-title":"Status of membrane lipids and amino acid transport in morphological mutants of Candida albicans","volume":"35","author":"Koul","year":"1995","journal-title":"Biochem Mol Biol Int"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.2174\/1875039700801010005"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1139\/o75-177"},{"key":"r21","first-page":"30","article-title":"Influence of lipid composition on the sensitivity of Candida albicans to antifungal agents","volume":"26","author":"Mago","year":"1989","journal-title":"Indian J Biochem Biophys"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.3.675-684.2004"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4333-4340.2003"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1080\/13693780500107554"},{"key":"r25","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.15.9809"},{"key":"r26","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00861-07"},{"key":"r27","first-page":"305","article-title":"Lipid content and ESR determination of plasma membrane order parameter in Candida albicans sterol mutants","volume":"32","author":"Pesti","year":"1985","journal-title":"Acta Microbiol Hung"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.8.3442-3452.2005"},{"key":"r29","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.025718-0"},{"key":"r30","doi-asserted-by":"publisher","DOI":"10.1128\/EC.5.3.488-498.2006"},{"key":"r31","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(78)90407-1"},{"key":"r32","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2010.0041"},{"key":"r33","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.160077797"},{"key":"r34","doi-asserted-by":"publisher","DOI":"10.2174\/138945005774912771"},{"key":"r35","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2006.07.020"},{"key":"r36","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2005.11.008"},{"key":"r37","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205375200"},{"key":"r38","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/006163-0"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.051086-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T11:18:31Z","timestamp":1605784711000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.051086-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11,1]]},"references-count":38,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2011,11,1]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.051086-0","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11,1]]}}}