{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T14:49:43Z","timestamp":1789915783508,"version":"4.0.1"},"reference-count":33,"publisher":"Microbiology Society","issue":"10","funder":[{"name":"EU","award":["HEALTH-F3-2009-222983"],"award-info":[{"award-number":["HEALTH-F3-2009-222983"]}]},{"DOI":"10.13039\/501100004681","name":"Higher Education Commission (HEC) Pakistan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004681","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,10,1]]},"abstract":"\n The human pathogen\n Streptococcus pneumoniae<\/jats:italic>\n harbours many genes encoding phosphotransferase systems and sugar ABC (ATP-binding cassette) transporters, including systems for the utilization of the \u03b2-glucoside sugar cellobiose. In this study, we show that the transcriptional regulator CelR, which has previously been found to be important for pneumococcal virulence, activates the expression of the cellobiose-utilization gene cluster (\n cel<\/jats:italic>\n locus) of\n S. pneumoniae<\/jats:italic>\n . Expression directed by the two promoters present in the\n cel<\/jats:italic>\n locus was increased in the presence of cellobiose as sole carbon source in the medium, while expression decreased in the presence of glucose in the medium. Furthermore, we have predicted a 22 bp putative CelR regulatory site (5\u2032-YTTTCCWTAWCAWTWAGGAAAA-3\u2032) in the promoters of\n celA<\/jats:italic>\n and\n celB<\/jats:italic>\n , and\n in silico<\/jats:italic>\n analysis showed that it is highly conserved in other pathogenic streptococci as well. Promoter truncations of\n celA<\/jats:italic>\n and\n celB<\/jats:italic>\n , where the half or full CelR regulatory site was deleted, confirmed that the CelR-binding site in P\n celA<\/jats:italic>\n and P\n celB<\/jats:italic>\n is functional. Transcriptome studies with the\n celR<\/jats:italic>\n mutant and in\n silico<\/jats:italic>\n prediction of the CelR regulatory site in the entire D39 genome sequence show that the\n cel<\/jats:italic>\n locus is the only cluster of genes under the direct control of CelR. Therefore, CelR is a regulator dedicated to the cellobiose-dependent transcriptional activation of the\n cel<\/jats:italic>\n locus.\n <\/jats:p>","DOI":"10.1099\/mic.0.051359-0","type":"journal-article","created":{"date-parts":[[2011,7,21]],"date-time":"2011-07-21T20:47:35Z","timestamp":1311281255000},"page":"2854-2861","source":"Crossref","is-referenced-by-count":33,"title":["CelR-mediated activation of the cellobiose-utilization gene cluster in Streptococcus pneumoniae"],"prefix":"10.1099","volume":"157","author":[{"given":"Sulman","family":"Shafeeq","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomas G.","family":"Kloosterman","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar P.","family":"Kuipers","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","first-page":"28","article-title":"Fitting a mixture model by expectation maximization to discover motifs in biopolymers","volume":"2","author":"Bailey","year":"1994","journal-title":"Proc Int Conf Intell Syst Mol Biol"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)00938-7"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1955"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00024-06"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00584.x"},{"key":"r7","first-page":"1389","article-title":"Large-scale identification of serotype 4 Streptococcus pneumoniae virulence factors","volume":"45","author":"Hava","year":"2002","journal-title":"Mol Microbiol"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1871"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.26.14367"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.13.4246-4253.2004"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1014.2009.00564.x"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28521-0"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M601661200"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01148-06"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02335.x"},{"key":"r17a","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1007\/s004380050315","article-title":"A general system for generating unlabelled gene replacements in bacterial chromosomes","volume":"253","author":"Leenhouts","year":"1996","journal-title":"Mol Gen Genet"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.6.1228"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M010192200"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1159\/000096463"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01170-06"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro771"},{"key":"r22","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1099\/0022-1317-51-2-98","article-title":"The pneumococcus: carriage, disease and conjugate vaccines","volume":"51","author":"Obaro","year":"2002","journal-title":"J Med Microbiol"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2005.00246.x"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.9.4850"},{"key":"r25","doi-asserted-by":"publisher","DOI":"10.1039\/c1mt00030f"},{"key":"r26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07758.x"},{"key":"r27","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2008.04.002"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2008.10.006"},{"key":"r29","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1128\/am.29.6.807-813.1975","article-title":"Improved medium for lactic streptococci and their bacteriophages","volume":"29","author":"Terzaghi","year":"1975","journal-title":"Appl Microbiol"},{"key":"r30","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01725.x"},{"key":"r31","first-page":"241","article-title":"MicroPreP: a cDNA microarray data pre-processing framework","volume":"2","author":"van Hijum","year":"2003","journal-title":"Appl Bioinformatics"},{"key":"r32","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(01)00267-6"},{"key":"r33","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01641-08"},{"key":"r34","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01013-06"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.051359-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,28]],"date-time":"2021-11-28T08:46:15Z","timestamp":1638089175000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.051359-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10,1]]},"references-count":33,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2011,10,1]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.051359-0","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10,1]]}}}