{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T07:19:11Z","timestamp":1791098351615,"version":"4.1.0"},"reference-count":37,"publisher":"Microbiology Society","issue":"2","funder":[{"name":"University of Kentucky Faculty Research Support"},{"name":"PHS","award":["R01 AI48491"],"award-info":[{"award-number":["R01 AI48491"]}]},{"name":"PHS","award":["R21 AI083861"],"award-info":[{"award-number":["R21 AI083861"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,2,1]]},"abstract":"<jats:p>YadB and YadC are putative trimeric autotransporters present only in the plague bacterium <jats:italic>Yersinia pestis<\/jats:italic> and its evolutionary predecessor, <jats:italic>Yersinia pseudotuberculosis<\/jats:italic>. Previously, <jats:italic>yadBC<\/jats:italic> was found to promote invasion of epithelioid cells by <jats:italic>Y. pestis<\/jats:italic> grown at 37 \u00b0C. In this study, we found that <jats:italic>yadBC<\/jats:italic> also promotes uptake of 37 \u00b0C-grown <jats:italic>Y. pestis<\/jats:italic> by mouse monocyte\/macrophage cells. We tested whether <jats:italic>yadBC<\/jats:italic> might be required for lethality of the systemic stage of plague in which the bacteria would be pre-adapted to mammalian body temperature before colonizing internal organs and found no requirement for early colonization or growth over 3 days. We tested the hypothesis that YadB and YadC function on ambient temperature-grown <jats:italic>Y. pestis<\/jats:italic> in the flea vector or soon after infection of the dermis in bubonic plague. We found that <jats:italic>yadBC<\/jats:italic> did not promote uptake by monocyte\/macrophage cells if the bacteria were grown at 28 \u00b0C, nor was there a role of <jats:italic>yadBC<\/jats:italic> in colonization of fleas by <jats:italic>Y. pestis<\/jats:italic> grown at 21 \u00b0C. However, the presence of <jats:italic>yadBC<\/jats:italic> did promote recoverability of the bacteria from infected skin for 28 \u00b0C-grown <jats:italic>Y. pestis<\/jats:italic>. Furthermore, the gene for the proinflammatory chemokine CXCL1 was upregulated in expression if the infecting <jats:italic>Y. pestis<\/jats:italic> lacked <jats:italic>yadBC<\/jats:italic> but not if <jats:italic>yadBC<\/jats:italic> was present. Also, <jats:italic>yadBC<\/jats:italic> was not required for recoverability if the bacteria were grown at 37 \u00b0C. These findings imply that thermally induced virulence properties dominate over effects of <jats:italic>yadBC<\/jats:italic> during plague but that <jats:italic>yadBC<\/jats:italic> has a unique function early after transmission of <jats:italic>Y. pestis<\/jats:italic> to skin.<\/jats:p>","DOI":"10.1099\/mic.0.073205-0","type":"journal-article","created":{"date-parts":[[2013,11,13]],"date-time":"2013-11-13T02:44:28Z","timestamp":1384310668000},"page":"396-405","source":"Crossref","is-referenced-by-count":7,"title":["Temperature-dependence of yadBC phenotypes in Yersinia pestis"],"prefix":"10.1099","volume":"160","author":[{"given":"Annette M.","family":"Uittenbogaard","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536-0298, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanya","family":"Myers-Morales","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536-0298, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda A.","family":"Gorman","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536-0298, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin","family":"Welsh","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536-0298, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Wulff","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536-0298, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. 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