{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:43:20Z","timestamp":1792806200783,"version":"4.5.0"},"reference-count":54,"publisher":"Microbiology Society","issue":"3","funder":[{"name":"National Science Foundation","award":["NSF-EF-0913042"],"award-info":[{"award-number":["NSF-EF-0913042"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,3,1]]},"abstract":"<jats:p>\n                    Shiga toxin (\n                    <jats:italic>stx<\/jats:italic>\n                    )-producing\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    O157\u200a:\u200aH7 is a prominent food-borne pathogen. Symptoms in human infections range from asymptomatic to haemorrhagic colitis and haemolytic uraemic syndrome, and there is a need for methods that yield information that can be used to better predict clinical and epidemiological outcomes. IS\n                    <jats:italic>629<\/jats:italic>\n                    is an insertion sequence notable for its prevalence and variable distribution in the chromosome of\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157\u200a:\u200aH7, which has been exploited for subtyping and strain characterization. In particular, IS\n                    <jats:italic>629<\/jats:italic>\n                    distribution is closely aligned with the major phylogenetic lineages that are known to be distinctive in their genome structure and virulence potential. In the present study, a comprehensive subtyping method in which IS\n                    <jats:italic>629<\/jats:italic>\n                    -typing was combined with\n                    <jats:italic>stx<\/jats:italic>\n                    genotyping was developed using a conventional PCR approach. This method consisted of a set of 32 markers based on the unique distribution of IS\n                    <jats:italic>629<\/jats:italic>\n                    in the three major phylogenetic lineages of\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157\u200a:\u200aH7 and six additional markers to determine the\n                    <jats:italic>stx<\/jats:italic>\n                    genotype, a key virulence signature associated with each lineage. The analysis of IS\n                    <jats:italic>629<\/jats:italic>\n                    loci variation with the 32 markers allowed us to determine the IS\n                    <jats:italic>629<\/jats:italic>\n                    distribution profile (IDP), phylogenetic lineage and genetic relatedness of the 31\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157\u200a:\u200aH7 strains examined. An association between IDP typing and\n                    <jats:italic>stx<\/jats:italic>\n                    genotype was observed. The use of both IDP and the\n                    <jats:italic>stx<\/jats:italic>\n                    genotype for strain characterization provided confirmative and complementary data in support of lineage placement of closely related strains. In addition, IS\n                    <jats:italic>629<\/jats:italic>\n                    \/\n                    <jats:italic>stx<\/jats:italic>\n                    profiles were in agreement with strain segregation based on LSPA-6 (lineage-specific polymorphism assay) and PFGE subtyping, demonstrating its potential as a subtyping and strain tracking method.\n                  <\/jats:p>","DOI":"10.1099\/mic.0.073437-0","type":"journal-article","created":{"date-parts":[[2014,1,15]],"date-time":"2014-01-15T01:38:42Z","timestamp":1389749922000},"page":"502-513","source":"Crossref","is-referenced-by-count":16,"title":["Phylogenetic characterization of Escherichia coli O157\u200a:\u200aH7 based on IS629 distribution and Shiga toxin genotype"],"prefix":"10.1099","volume":"160","author":[{"given":"Eliot","family":"Stanton","sequence":"first","affiliation":[{"name":"Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, 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