{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:51:49Z","timestamp":1788871909215,"version":"build-2803163510"},"reference-count":35,"publisher":"Microbiology Society","issue":"6","funder":[{"name":"DBT","award":["BT\/PR11453\/BID\/07\/296\/2009"],"award-info":[{"award-number":["BT\/PR11453\/BID\/07\/296\/2009"]}]},{"name":"Biotechnology Unit, Aligarh Muslim University"},{"name":"Department of Biotechnology (DBT), Government of India"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,6,1]]},"abstract":"<jats:p>\n                    We studied the molecular mechanism of resistance in extended-spectrum \u03b2-lactamase (ESBL)-producing\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    isolated from a neonatal intensive care unit (NICU) of one of the hospitals in North India. A total of 3000 clinical samples were collected from a NICU (January 2009 to February 2011), of which 523 strains were\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    positive and 262 of them were ESBL-producing\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    strains. All of the ESBL-producing clinical isolates were susceptible to carbapenems. However, the majority of the clinical isolates (30\u201396\u200a%) were resistant to a wide range of antibiotics including antibiotic\/inhibitor combinations. The MIC values confirmed that these isolates were highly resistant to cephalosporins and aztreonam. In the 262 ESBL-producing\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolates, 15 different enterobacterial repetitive intergenic consensus (ERIC)-PCR-typed phylogenetic groups were identified and reconfirmed by PFGE. Characterization of plasmids from each representative member of these phylogenetic groups revealed the presence of three plasmids of different sizes. Conjugation experiments confirmed the presence of different resistance markers only on the 154 kb plasmid. PCR amplification and sequence analysis revealed that\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M-3<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM-1<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>SHV-1<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA-1<\/jats:sub>\n                    and\n                    <jats:italic>armA<\/jats:italic>\n                    were the predominant resistance markers. Plasmid-replicon typing showed that IncI1-I\u03b3 and IncFIA-FIB types are the most prevalent. This study shows the co-existence of multiple ESBL-encoding genes and their polyclonal dissemination among\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    clinical isolates in the NICU of a North Indian hospital.\n                  <\/jats:p>","DOI":"10.1099\/mic.0.075762-0","type":"journal-article","created":{"date-parts":[[2014,3,31]],"date-time":"2014-03-31T20:15:46Z","timestamp":1396296946000},"page":"1153-1161","source":"Crossref","is-referenced-by-count":7,"title":["Prevalence of IncI1-I\u03b3 and IncFIA-FIB type plasmids in extended-spectrum \u03b2-lactamase-producing Klebsiella pneumoniae strains isolated from the NICU of a North Indian hospital"],"prefix":"10.1099","volume":"160","author":[{"given":"Saeedut Zafar","family":"Ali","sequence":"first","affiliation":[{"name":"Medical Microbiology and Molecular Biology Lab, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed Manazir","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, J. 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Medical College and Hospital, Aligarh Muslim University, Aligarh 202002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asad U.","family":"Khan","sequence":"additional","affiliation":[{"name":"Medical Microbiology and Molecular Biology Lab, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","first-page":"179","article-title":"Rep-PCR reveals a high genetic homogeneity among Ugandan isolates of Xanthomonas campestris pv musacearum","volume":"6","author":"Aritua","year":"2007","journal-title":"Afr J Biotechnol"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl077"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"r4","article-title":"Performance Standards for Antimicrobial Susceptibility Testing; M100-S21","year":"2011"},{"key":"r5","volume-title":"L\u2019Antibiogramme","author":"Courvalin","year":"1985"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.5.1843-1849.2003"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.2.491-496.2004"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.03057.x"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00879-06"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.8.2565-2571.2003"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.005850-0"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.8.2166-2169.2000"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1016\/j.patbio.2008.07.025"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1016\/S1995-7645(12)60003-0"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp256"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01099-12"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(84)90063-5"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.12.003"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1086\/319757"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp068"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2009.03.034"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822010000300009"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-9-97"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01773-10"},{"key":"r25","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4264-4269.2003"},{"key":"r26","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl317"},{"key":"r27","first-page":"392","article-title":"Extended-spectrum \u03b2-lactamase producing Klebsiella pneumoniae from blood cultures in Puducherry, India","volume":"134","author":"Parveen","year":"2011","journal-title":"Indian J Med Res"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01247-10"},{"key":"r29","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.06.007"},{"key":"r30","doi-asserted-by":"publisher","DOI":"10.4172\/1948-5948.1000044"},{"key":"r31","doi-asserted-by":"publisher","DOI":"10.1093\/tropej\/fmp060"},{"key":"r32","first-page":"211","article-title":"Prevalence of antimicrobial drug resistance of Klebsiella pneumoniae in India","volume":"1","author":"Sikarwar","year":"2011","journal-title":"Int J Biosci Biochem Bioinform"},{"key":"r33","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"},{"key":"r34","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.7.2242-2248.2003"},{"key":"r35","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2003.07.011"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.075762-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T12:18:52Z","timestamp":1605788332000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.075762-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,1]]},"references-count":35,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,6,1]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.075762-0","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,1]]}}}