{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T21:41:04Z","timestamp":1788212464469,"version":"build-2803163510"},"reference-count":59,"publisher":"Microbiology Society","issue":"8","funder":[{"name":"Netherlands Technology Foundation","award":["10467"],"award-info":[{"award-number":["10467"]}]},{"name":"Netherlands Technology Foundation","award":["11992"],"award-info":[{"award-number":["11992"]}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,8,1]]},"abstract":"<jats:p>The rapid emergence of multidrug-resistant (MDR) bacterial pathogens poses a major threat for human health. In recent years, genome sequencing has unveiled many poorly expressed antibiotic clusters in actinomycetes. Here, we report a well-defined ecological collection of &gt;800 actinomycetes obtained from sites in the Himalaya and Qinling mountains, and we used these in a concept study to see how efficiently antibiotics can be elicited against MDR pathogens isolated recently from the clinic. Using 40 different growth conditions, 96 actinomycetes were identified \u2013 predominantly<jats:italic>Streptomyces<\/jats:italic>\u2013 that produced antibiotics with efficacy against the MDR clinical isolates referred to as ESKAPE pathogens:<jats:italic>Enterococcus faecium<\/jats:italic>,<jats:italic>Staphylococcus aureus<\/jats:italic>,<jats:italic>Klebsiella pneumoniae<\/jats:italic>,<jats:italic>Acinetobacter baumannii<\/jats:italic>,<jats:italic>Pseudomonas aeruginosa<\/jats:italic>and\/or<jats:italic>Enterobacter cloacae<\/jats:italic>. Antimicrobial activities that fluctuated strongly with growth conditions were correlated with specific compounds, including borrelidin, resistomycin, carbomethoxy-phenazine, and 6,7,8- and 5,6,8-trimethoxy-3-methylisocoumarin, of which the latter was not described previously. Our work provided insights into the potential of actinomycetes as producers of drugs with efficacy against clinical isolates that have emerged recently and also underlined the importance of targeting a specific pathogen.<\/jats:p>","DOI":"10.1099\/mic.0.078295-0","type":"journal-article","created":{"date-parts":[[2014,5,3]],"date-time":"2014-05-03T05:22:42Z","timestamp":1399094562000},"page":"1714-1725","source":"Crossref","is-referenced-by-count":96,"title":["Eliciting antibiotics active against the ESKAPE pathogens in a collection of actinomycetes isolated from mountain soils"],"prefix":"10.1099","volume":"160","author":[{"given":"Hua","family":"Zhu","sequence":"first","affiliation":[{"name":"Molecular Biotechnology, Institute of Biology, Molecular Biotechnology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasper","family":"Swierstra","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Centre, \u2018s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changsheng","family":"Wu","sequence":"additional","affiliation":[{"name":"Molecular Biotechnology, Institute of Biology, Molecular Biotechnology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevi\u00e8ve","family":"Girard","sequence":"additional","affiliation":[{"name":"Molecular Biotechnology, Institute of Biology, Molecular Biotechnology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Hae","family":"Choi","sequence":"additional","affiliation":[{"name":"Natural Products Laboratory, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem","family":"van Wamel","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Centre, \u2018s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie K.","family":"Sandiford","sequence":"additional","affiliation":[{"name":"Molecular Biotechnology, Institute of Biology, Molecular Biotechnology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles P.","family":"van Wezel","sequence":"additional","affiliation":[{"name":"Molecular Biotechnology, Institute of Biology, Molecular Biotechnology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201200619"},{"key":"r2","first-page":"125","article-title":"Antimicrobials from actinomycetes: back to the future","volume":"2","author":"Baltz","year":"2007","journal-title":"Microbe"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2008.04.008"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1038\/417141a"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1086\/533590"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2004.03.022"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1934677100"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2009.00206.x"},{"key":"r57","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1038\/nrmicro3178","article-title":"Bacterial solutions to multicellularity; a tale of biofilms, filaments and fruiting bodies","volume":"12","author":"Claessen","year":"2014","journal-title":"Nat Rev Microbiol"},{"key":"r9","volume-title":"Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria; Approved Standard","year":"2004"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/011940-0"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2012.06.013"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evt082"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1016\/S1526-0550(02)00003-3"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1126\/science.1176667"},{"key":"r58","doi-asserted-by":"crossref","first-page":"130073","DOI":"10.1098\/rsob.130073","article-title":"A novel taxonomic marker that discriminates between morphologically complex actinomycetes","volume":"3","author":"Girard","year":"2013","journal-title":"Open Biol"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01169-07"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1007\/s10637-011-9700-y"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.3630150604"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1016\/0385-6380(87)90108-7"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.3209\/saj.3_95"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1007\/BF01574078"},{"key":"r21","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780195150667.001.0001","volume-title":"Streptomyces in Nature and Medicine: The Antibiotic Makers","author":"Hopwood","year":"2007"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1007\/BF02877385"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-251-9_3"},{"key":"r24","volume-title":"Practical Streptomyces Genetics","author":"Kieser","year":"2000"},{"key":"r25","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2010.02.001"},{"key":"r26","doi-asserted-by":"publisher","DOI":"10.1086\/499367"},{"key":"r27","doi-asserted-by":"publisher","DOI":"10.1038\/202928a0"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010287600557"},{"key":"r29","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(92)90603-M"},{"key":"r30","doi-asserted-by":"publisher","DOI":"10.3209\/saj.7_100"},{"key":"r31","doi-asserted-by":"publisher","DOI":"10.4103\/0253-7613.24625"},{"key":"r32","doi-asserted-by":"publisher","DOI":"10.1007\/BF00343181"},{"key":"r33","first-page":"96","article-title":"Isolation, screening and characterization of antibiotic-producing actinomycetes from rhizosphere region of different plants from a farm of Sungai Ramal Luar, Malaysia","volume":"2","author":"Ng","year":"2012","journal-title":"J Adv Biomed Pathobiol Res"},{"key":"r34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.07020.x"},{"key":"r35","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00204-08"},{"key":"r36","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-289673-6.50007-5"},{"key":"r37","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2201"},{"key":"r38","doi-asserted-by":"publisher","DOI":"10.1086\/533452"},{"key":"r39","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05319.x"},{"key":"r40","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2008.83"},{"key":"r41","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.47.136"},{"key":"r42","doi-asserted-by":"publisher","DOI":"10.1002\/mrc.1270190203"},{"key":"r43","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2010.534993"},{"key":"r44","doi-asserted-by":"publisher","DOI":"10.1038\/ja.2010.78"},{"key":"r45","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1515\/znc-2002-9-1028","article-title":"Effects of polyphenolic anthrone derivatives, resistomycin and hypercin, on apoptosis in human megakaryoblastic leukemia CMK-7 cell line","volume":"57","author":"Shiono","year":"2002","journal-title":"Z Naturforsch C"},{"key":"r46","doi-asserted-by":"publisher","DOI":"10.3923\/biotech.2006.217.221"},{"key":"r47","doi-asserted-by":"publisher","DOI":"10.1086\/524891"},{"key":"r48","doi-asserted-by":"publisher","DOI":"10.1128\/JB.06370-11"},{"key":"r49","doi-asserted-by":"publisher","DOI":"10.2323\/jgam.49.141"},{"key":"r50","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msr121"},{"key":"r51","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2007.08.001"},{"key":"r52","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.2013.838218"},{"key":"r53","doi-asserted-by":"publisher","DOI":"10.1039\/c1np00003a"},{"key":"r54","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(09)04805-8"},{"key":"r55","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-011-1260-5"},{"key":"r59","doi-asserted-by":"crossref","first-page":"671","DOI":"10.7164\/antibiotics.50.671","article-title":"Borrelidin is an angiogenesis inhibitor; disruption of angiogenic capillary vessels in a rat aorta matrix culture model","volume":"50","author":"Wakabayashi","year":"1997","journal-title":"J Antibiot (Tokyo)"},{"key":"r56","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-013-1309-z"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.078295-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,26]],"date-time":"2024-05-26T09:24:16Z","timestamp":1716715456000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.078295-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,1]]},"references-count":59,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,8,1]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.078295-0","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,1]]}}}