{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T07:05:27Z","timestamp":1768547127504,"version":"3.49.0"},"reference-count":59,"publisher":"Microbiology Society","issue":"8","funder":[{"DOI":"10.13039\/501100005416","name":"Norwegian Research Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["SI 848\/4-1"],"award-info":[{"award-number":["SI 848\/4-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,8,1]]},"abstract":"<jats:p>Global warming is moving more and more into the public consciousness. Besides the commonly mentioned carbon dioxide and methane, nitrous oxide (N<jats:sub>2<\/jats:sub>O) is a powerful greenhouse gas in addition to its contribution to depletion of stratospheric ozone. The increasing concern about N<jats:sub>2<\/jats:sub>O emission has focused interest on underlying microbial energy-converting processes and organisms harbouring N<jats:sub>2<\/jats:sub>O reductase (NosZ), such as denitrifiers and ammonifiers of nitrate and nitrite. Here, the epsilonproteobacterial model organism<jats:italic>Wolinella succinogenes<\/jats:italic>is investigated with regard to its capacity to produce and consume N<jats:sub>2<\/jats:sub>O during growth by anaerobic nitrate ammonification. This organism synthesizes an unconventional cytochrome<jats:italic>c<\/jats:italic>nitrous oxide reductase (<jats:italic>c<\/jats:italic>NosZ), which is encoded by the first gene of an atypical<jats:italic>nos<\/jats:italic>gene cluster. However,<jats:italic>W. succinogenes<\/jats:italic>lacks a nitric oxide (NO)-producing nitrite reductase of the NirS- or NirK-type as well as an NO reductase of the Nor-type. Using a robotized incubation system, the wild-type strain and suitable mutants of<jats:italic>W. succinogenes<\/jats:italic>that either produced or lacked<jats:italic>c<\/jats:italic>NosZ were analysed as to their production of NO, N<jats:sub>2<\/jats:sub>O and N<jats:sub>2<\/jats:sub>in both nitrate-sufficient and nitrate-limited growth medium using formate as electron donor. It was found that cells growing in nitrate-sufficient medium produced small amounts of N<jats:sub>2<\/jats:sub>O, which derived from nitrite and, most likely, from the presence of NO. Furthermore, cells employing<jats:italic>c<\/jats:italic>NosZ were able to reduce N<jats:sub>2<\/jats:sub>O to N<jats:sub>2<\/jats:sub>. This reaction, which was fully inhibited by acetylene, was also observed after adding N<jats:sub>2<\/jats:sub>O to the culture headspace. The results indicate that<jats:italic>W. succinogenes<\/jats:italic>cells are competent in N<jats:sub>2<\/jats:sub>O and N<jats:sub>2<\/jats:sub>production despite being correctly grouped as respiratory nitrate ammonifiers. N<jats:sub>2<\/jats:sub>O production is assumed to result from NO detoxification and nitrosative stress defence, while N<jats:sub>2<\/jats:sub>O serves as a terminal electron acceptor in anaerobic respiration. The ecological implications of these findings are discussed.<\/jats:p>","DOI":"10.1099\/mic.0.079293-0","type":"journal-article","created":{"date-parts":[[2014,4,30]],"date-time":"2014-04-30T03:40:04Z","timestamp":1398829204000},"page":"1749-1759","source":"Crossref","is-referenced-by-count":24,"title":["Production and consumption of nitrous oxide in nitrate-ammonifying Wolinella succinogenes cells"],"prefix":"10.1099","volume":"160","author":[{"given":"Monique","family":"Luckmann","sequence":"first","affiliation":[{"name":"Microbial Energy Conversion and Biotechnology, Department of Biology, Technische Universit\u00e4t Darmstadt, Schnittspahnstra\u00dfe 10, 64287 Darmstadt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Mania","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Chr Falsens vei 1, N1432 \u00c5s, 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