{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T11:44:21Z","timestamp":1771328661166,"version":"3.50.1"},"reference-count":70,"publisher":"Microbiology Society","issue":"12","funder":[{"name":"Natural Science and Technology Major Project of China","award":["2013ZX10004803"],"award-info":[{"award-number":["2013ZX10004803"]}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2010CB530200\/2010CB530205"],"award-info":[{"award-number":["2010CB530200\/2010CB530205"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31170161"],"award-info":[{"award-number":["31170161"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81371767"],"award-info":[{"award-number":["81371767"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"<jats:p>The obligate intracellular Gram-negative bacterium <jats:italic>Coxiella burnetii<\/jats:italic> causes Q fever, a worldwide zoonosis. Here we labelled <jats:italic>Cox<\/jats:italic>\n                  <jats:italic>. burnetii<\/jats:italic> with biotin and used biotin-streptavidin affinity chromatography to isolate surface-exposed proteins (SEPs). Using two-dimensional electrophoresis combined with mass spectrometry, we identified 37 proteins through bioinformatics analysis. Thirty SEPs expressed in <jats:italic>Escherichia coli<\/jats:italic> (recombinant SEPs, rSEPs) were used to generate microarrays, which were probed with sera from mice experimentally infected with <jats:italic>Cox. burnetii<\/jats:italic> or sera from Q fever patients. Thirteen rSEPs were recognized as seroreactive, and the majority reacted with at least 50\u200a% of the sera from mice infected with <jats:italic>Cox. burnetii<\/jats:italic> but not with sera from mice infected with <jats:italic>Rickettsia rickettsii<\/jats:italic>, <jats:italic>R. heilongjiangensis,<\/jats:italic> or <jats:italic>R. typhi.<\/jats:italic> Further, 13 proteins that reacted with sera from patients with Q fever did not react with sera from patients with brucellosis or mycoplasma pneumonia. Our results suggest that these seroreactive SEPs have potential as serodiagnostic antigens or as subunit vaccine antigens against Q fever.<\/jats:p>","DOI":"10.1099\/mic.0.082131-0","type":"journal-article","created":{"date-parts":[[2014,10,9]],"date-time":"2014-10-09T04:42:59Z","timestamp":1412829779000},"page":"2718-2731","source":"Crossref","is-referenced-by-count":17,"title":["Serological characterization of surface-exposed proteins of Coxiella burnetii"],"prefix":"10.1099","volume":"160","author":[{"given":"Jun","family":"Jiao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolu","family":"Xiong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Medical and Pharmaceutical Biotechnology, Huadong Research Institute for Medicine and Biotechniques, Nanjing 210002, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenping","family":"Gong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changsong","family":"Duan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomei","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bohai","family":"Wen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"704","DOI":"10.5694\/j.1326-5377.1994.tb125909.x","article-title":"Vaccine prophylaxis of Q fever. 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