{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:02:13Z","timestamp":1791295333347,"version":"4.1.0"},"reference-count":49,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,5,1]]},"DOI":"10.1099\/mic.0.2006\/004200-0","type":"journal-article","created":{"date-parts":[[2007,4,26]],"date-time":"2007-04-26T18:57:57Z","timestamp":1177613877000},"page":"1593-1603","source":"Crossref","is-referenced-by-count":64,"title":["The outer membrane secretin PilQ from Neisseria meningitidis binds DNA"],"prefix":"10.1099","volume":"153","author":[{"given":"Reza","family":"Assalkhou","sequence":"first","affiliation":[{"name":"Centre for Molecular Biology and Neuroscience and Institute of Microbiology, Rikshospitalet-Radiumhospitalet Medical Centre, Oslo, Norway"},{"name":"Centre for Molecular Biology and Neuroscience and Institute of Microbiology, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seetha","family":"Balasingham","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology and Neuroscience and Institute of Microbiology, Rikshospitalet-Radiumhospitalet Medical Centre, Oslo, Norway"},{"name":"Centre for Molecular Biology and Neuroscience and Institute of Microbiology, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard F","family":"Collins","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences, The University of Manchester, Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester M1 7DN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan A","family":"Frye","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology and Neuroscience and Institute of Microbiology, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonje","family":"Davidsen","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology and Neuroscience and Institute of Microbiology, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afsaneh V","family":"Benam","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology and Neuroscience and Institute of Microbiology, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnar","family":"Bj\u00f8r\u00e5s","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology and Neuroscience and Institute of Microbiology, Rikshospitalet-Radiumhospitalet Medical Centre, Oslo, Norway"},{"name":"Centre for Molecular Biology and Neuroscience and Institute of Microbiology, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy P","family":"Derrick","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences, The University of Manchester, Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester M1 7DN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tone","family":"T\u00f8njum","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology and Neuroscience and Institute of Microbiology, Rikshospitalet-Radiumhospitalet Medical Centre, Oslo, Norway"},{"name":"Centre for Molecular Biology and Neuroscience and Institute of Microbiology, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1046\/j.1365-2958.2002.03193.x","article-title":"Competence for natural transformation in Neisseria gonorrhoeae : components of DNA binding and uptake linked to type IV pilus expression","volume":"46","author":"Aas","year":"2002","journal-title":"Mol Microbiol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1084\/jem.20050112","article-title":"A chromosomally integrated bacteriophage in invasive meningococci","volume":"201","author":"Bille","year":"2005","journal-title":"J Exp Med"},{"key":"R3","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1046\/j.1365-2958.1998.00677.x","article-title":"Formation of oligomeric rings by XcpQ and PilQ, which are involved in protein transport across the outer membrane of Pseudomonas aeruginosa","volume":"27","author":"Bitter","year":"1998","journal-title":"Mol Microbiol"},{"key":"R4","first-page":"765","article-title":"Electron microscopical and serological characteristics associated with colony type in Moraxella nonliquefaciens . Acta Pathol Microbiol Scand [B]","volume":"78","author":"B\u00f8vre","year":"1970","journal-title":"Microbiol Immunol"},{"key":"R5","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"R6","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/0042-6822(74)90150-0","article-title":"The adsorption of Pseudomonas aeruginosa pilus-dependent bacteriophages to a host mutant with nonretractile pili","volume":"58","author":"Bradley","year":"1974","journal-title":"Virology"},{"key":"R7","first-page":"155","article-title":"Uses of pili in gonorrhea control: role of bacterial pili in disease, purification and properties of gonococcal pili, and progress in the development of a gonococcal pilus vaccine for gonorrhoeae","volume-title":"Immunobiology of Neisseria gonorrhoeae","author":"Brinton","year":"1978"},{"key":"R8","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1111\/apm.1964.61.3.457","article-title":"Studies on transformation in moraxella and organisms assumed to be related to moraxella. 1. A method for quantitative transformation in moraxella and neisseria, with streplomycin resistance as the genetic marker","volume":"61","author":"B\u00f8vre","year":"1964","journal-title":"Acta Pathol Microbiol Scand"},{"key":"R9","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1111\/j.1365-2958.2006.05341.x","article-title":"A systematic genetic analysis in Neisseria meningitidis defines the Pil proteins required for assembly, functionality, stabilization and export of type IV pili","volume":"61","author":"Carbonnelle","year":"2006","journal-title":"Mol Microbiol"},{"key":"R10","doi-asserted-by":"crossref","first-page":"5117","DOI":"10.1128\/JB.180.19.5117-5122.1998","article-title":"Formation of single-stranded DNA during DNA transformation of Neisseria gonorrhoeae","volume":"180","author":"Chaussee","year":"1998","journal-title":"J Bacteriol"},{"key":"R11","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1038\/nrmicro844","article-title":"DNA uptake during bacterial transformation","volume":"2","author":"Chen","year":"2004","journal-title":"Nat Rev Microbiol"},{"key":"R12","doi-asserted-by":"crossref","first-page":"3160","DOI":"10.1128\/JB.183.10.3160-3168.2001","article-title":"ComE, a competence protein from Neisseria gonorrhoeae with DNA-binding activity","volume":"183","author":"Chen","year":"2001","journal-title":"J Bacteriol"},{"key":"R13","doi-asserted-by":"crossref","first-page":"3825","DOI":"10.1128\/JB.183.13.3825-3832.2001","article-title":"Analysis of the PilQ secretin from Neisseria meningitidis by transmission electron microscopy reveals a dodecameric quaternary structure","volume":"183","author":"Collins","year":"2001","journal-title":"J Bacteriol"},{"key":"R14","doi-asserted-by":"crossref","first-page":"2611","DOI":"10.1128\/JB.185.8.2611-2617.2003","article-title":"Three-dimensional structure of the Neisseria meningitidis secretin PilQ determined from negative-stain transmission electron microscopy","volume":"185","author":"Collins","year":"2003","journal-title":"J Bacteriol"},{"key":"R15","doi-asserted-by":"crossref","first-page":"39750","DOI":"10.1074\/jbc.M405971200","article-title":"Structure of the Neisseria meningitidis outer membrane PilQ secretin complex at 12\u2005A\u030a resolution","volume":"279","author":"Collins","year":"2004","journal-title":"J Biol Chem"},{"key":"R16","doi-asserted-by":"crossref","first-page":"18923","DOI":"10.1074\/jbc.M411603200","article-title":"Interaction with type IV pili induces structural changes in the bacterial outer membrane secretin PilQ","volume":"280","author":"Collins","year":"2005","journal-title":"J Biol Chem"},{"key":"R17","doi-asserted-by":"crossref","first-page":"2801","DOI":"10.1128\/JB.187.8.2801-2809.2005","article-title":"Antimutator role of DNA glycosylase MutY in pathogenic Neisseria species","volume":"187","author":"Davidsen","year":"2005","journal-title":"J Bacteriol"},{"key":"R18","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1111\/j.1365-2958.1995.18050975.x","article-title":"The product of the pilQ gene is essential for the biogenesis of type IV pili in Neisseria gonorrhoeae","volume":"18","author":"Drake","year":"1995","journal-title":"Mol Microbiol"},{"key":"R19","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1128\/IAI.5.1.98-102.1972","article-title":"Classification of Neisseria meningitidis group B into distinct serotypes. I. Serological typing by a microbactericidal method","volume":"5","author":"Frasch","year":"1972","journal-title":"Infect Immun"},{"key":"R20","doi-asserted-by":"crossref","first-page":"3140","DOI":"10.1128\/AEM.67.7.3140-3148.2001","article-title":"Natural transformation in mesophilic and thermophilic bacteria: identification and characterization of novel, closely related competence genes in Acinetobacter sp. strain BD413 and Thermus thermophilus HB27","volume":"67","author":"Friedrich","year":"2001","journal-title":"Appl Environ Microbiol"},{"key":"R22","first-page":"525","article-title":"Electron microscopical and cultural features of Neisseria meningitidis competence variants","volume":"81","author":"Fr\u00f8holm","year":"1973","journal-title":"Acta Pathol Microbiol Scand [B] Microbiol Immunol"},{"key":"R21","doi-asserted-by":"crossref","first-page":"3751","DOI":"10.1099\/mic.0.2006\/000315-0","article-title":"Topology of the outer-membrane secretin PilQ from Neisseria meningitidis","volume":"152","author":"Frye","year":"2006","journal-title":"Microbiology"},{"key":"R23","doi-asserted-by":"crossref","first-page":"6982","DOI":"10.1073\/pnas.85.18.6982","article-title":"Identification and arrangement of the DNA sequence recognized in specific transformation of Neisseria gonorrhoeae","volume":"85","author":"Goodman","year":"1988","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R24","doi-asserted-by":"crossref","first-page":"4694","DOI":"10.1128\/JB.183.16.4694-4701.2001","article-title":"Requirement of novel competence genes pilT and pilU of Pseudomonas stutzeri for natural transformation and suppression of pilT deficiency by a hexahistidine tag on the type IV pilus protein PilAI","volume":"183","author":"Graupner","year":"2001","journal-title":"J Bacteriol"},{"key":"R25","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1111\/j.1365-2958.2005.04964.x","article-title":"Natural transformation of Neisseria gonorrhoeae : from DNA donation to homologous recombination","volume":"59","author":"Hamilton","year":"2006","journal-title":"Mol Microbiol"},{"key":"R26","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1111\/j.1600-0463.1998.tb05649.x","article-title":"Competence for natural transformation in Neisseria gonorrhoeae : a model system for studies of horizontal gene transfer","volume":"84","author":"Koomey","year":"1998","journal-title":"APMIS Suppl"},{"key":"R27","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1128\/JB.169.2.790-795.1987","article-title":"Cloning of the recA gene of Neisseria gonorrhoeae and construction of gonococcal recA mutants","volume":"169","author":"Koomey","year":"1987","journal-title":"J Bacteriol"},{"key":"R28","doi-asserted-by":"crossref","first-page":"6279","DOI":"10.1128\/IAI.71.11.6279-6291.2003","article-title":"Low-level pilin expression allows for substantial DNA transformation competence in Neisseria gonorrhoeae","volume":"71","author":"Long","year":"2003","journal-title":"Infect Immun"},{"key":"R29","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1007\/BF00276535","article-title":"Natural genetic transformation of Pseudomonas stutzeri by sand-adsorbed DNA","volume":"154","author":"Lorenz","year":"1990","journal-title":"Arch Microbiol"},{"key":"R30","first-page":"3165","article-title":"On the role of pili in transformation of Neisseria gonorrhoeae","volume":"130","author":"Mathis","year":"1984","journal-title":"J Gen Microbiol"},{"key":"R31","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1146\/annurev.micro.56.012302.160938","article-title":"Type IV pili and twitching motility","volume":"56","author":"Mattick","year":"2002","journal-title":"Annu Rev Microbiol"},{"key":"R32","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1046\/j.1365-2958.1998.01089.x","article-title":"Differential roles of homologous recombination pathways in Neisseria gonorrhoeae pilin antigenic variation, DNA transformation and DNA repair","volume":"30","author":"Mehr","year":"1998","journal-title":"Mol Microbiol"},{"key":"R33","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1111\/j.1574-6968.2002.tb11031.x","article-title":"Natural transformation of Pseudomonas stutzeri by single-stranded DNA requires type IV pili, competence state and comA","volume":"207","author":"Meier","year":"2002","journal-title":"FEMS Microbiol Lett"},{"key":"R34","doi-asserted-by":"crossref","first-page":"4691","DOI":"10.1073\/pnas.92.10.4691","article-title":"On the magnitude of the electrostatic contribution to ligand\u2013DNA interactions","volume":"92","author":"Misra","year":"1995","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R35","doi-asserted-by":"crossref","first-page":"8173","DOI":"10.1073\/pnas.96.14.8173","article-title":"Secretin PulD: association with pilot PulS, structure, and ion-conducting channel formation","volume":"96","author":"Nouwen","year":"1999","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R36","doi-asserted-by":"crossref","first-page":"2229","DOI":"10.1093\/emboj\/19.10.2229","article-title":"Domain structure of secretin PulD revealed by limited proteolysis and electron microscopy","volume":"19","author":"Nouwen","year":"2000","journal-title":"EMBO J"},{"key":"R37","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1046\/j.1365-2958.1998.01062.x","article-title":"Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development","volume":"30","author":"O'Toole","year":"1998","journal-title":"Mol Microbiol"},{"key":"R38","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/378032a0","article-title":"Structure of the fibre-forming protein pilin at 2.6\u2005A\u030a resolution","volume":"378","author":"Parge","year":"1995","journal-title":"Nature"},{"key":"R39","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/S0168-9525(00)02024-2","article-title":"emboss: the European Molecular Biology Open Software Suite","volume":"16","author":"Rice","year":"2000","journal-title":"Trends Genet"},{"key":"R40","volume-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"R41","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1128\/jb.184.4.919-927.2002","article-title":"Roles of the recJ and recN genes in homologous recombination and DNA repair pathways of Neisseria gonorrhoeae","volume":"184","author":"Skaar","year":"2002","journal-title":"J Bacteriol"},{"key":"R42","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1128\/JB.181.5.1395-1402.1999","article-title":"Natural competence for DNA transformation by Legionella pneumophila and its association with expression of type IV pili","volume":"181","author":"Stone","year":"1999","journal-title":"J Bacteriol"},{"key":"R43","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1084\/jem.134.4.886","article-title":"Studies on gonococcus infection. I. Pili and zones of adhesion: their relation to gonococcal growth patterns","volume":"134","author":"Swanson","year":"1971","journal-title":"J Exp Med"},{"key":"R44","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0378-1119(97)00018-8","article-title":"The pilus colonization factor of pathogenic neisserial species: organelle biogenesis and structure\/function relationships \u2013 a review","volume":"192","author":"T\u00f8njum","year":"1997","journal-title":"Gene"},{"key":"R45","first-page":"389","article-title":"Identification of Eikenella corrodens and Cardiobacterium hominis by genetic transformation","volume":"[B] 93","author":"T\u00f8njum","year":"1985","journal-title":"Acta Pathol Microbiol Immunol Scand"},{"key":"R46","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1111\/j.1365-2958.1995.tb02410.x","article-title":"Identification and characterization of pilG , a highly conserved pilus-assembly gene in pathogenic Neisseria","volume":"16","author":"T\u00f8njum","year":"1995","journal-title":"Mol Microbiol"},{"key":"R47","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1046\/j.1365-2958.1998.00910.x","article-title":"Structure and function of repetitive sequence elements associated with a highly polymorphic domain of the Neisseria meningitidis PilQ protein","volume":"29","author":"T\u00f8njum","year":"1998","journal-title":"Mol Microbiol"},{"key":"R48","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1128\/JB.187.4.1455-1464.2005","article-title":"DNA binding: a novel function of Pseudomonas aeruginosa type IV pili","volume":"187","author":"van Schaik","year":"2005","journal-title":"J Bacteriol"},{"key":"R49","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1046\/j.1365-2958.1999.01269.x","article-title":"The comP locus of Neisseria gonorrhoeae encodes a type IV prepilin that is dispensable for pilus biogenesis but essential for natural transformation","volume":"31","author":"Wolfgang","year":"1999","journal-title":"Mol Microbiol"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.2006\/004200-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,14]],"date-time":"2020-01-14T12:19:08Z","timestamp":1579004348000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.2006\/004200-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5,1]]},"references-count":49,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,5,1]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.2006\/004200-0","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5,1]]}}}