{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T03:22:15Z","timestamp":1793762535187,"version":"4.6.1"},"reference-count":46,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,8,1]]},"DOI":"10.1099\/mic.0.2007\/006312-0","type":"journal-article","created":{"date-parts":[[2007,7,27]],"date-time":"2007-07-27T20:23:08Z","timestamp":1185567788000},"page":"2518-2529","source":"Crossref","is-referenced-by-count":109,"title":["An Escherichia coli undecaprenyl-pyrophosphate phosphatase implicated in undecaprenyl phosphate recycling"],"prefix":"10.1099","volume":"153","author":[{"given":"Laura D.","family":"Tatar","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Infectious Diseases Research Group, Siebens Drake Research Institute, University of Western Ontario, London, Ontario N6A 5C1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina L.","family":"Marolda","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Infectious Diseases Research Group, Siebens Drake Research Institute, University of Western Ontario, London, Ontario N6A 5C1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew N.","family":"Polischuk","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Infectious Diseases Research Group, Siebens Drake Research Institute, University of Western Ontario, London, Ontario N6A 5C1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"van Leeuwen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Infectious Diseases Research Group, Siebens Drake Research Institute, University of Western Ontario, London, Ontario N6A 5C1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel A.","family":"Valvano","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Western Ontario, London, Ontario N6A 5C1, Canada"},{"name":"Department of Microbiology and Immunology, Infectious Diseases Research Group, Siebens Drake Research Institute, University of Western Ontario, London, Ontario N6A 5C1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/0968-0004(92)90424-8","article-title":"Topography of glycosylation reactions in the endoplasmic reticulum","volume":"17","author":"Abeijon","year":"1992","journal-title":"Trends Biochem Sci"},{"key":"R2","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/0003-9861(66)90056-7","article-title":"Biosynthesis of the peptidoglycan of bacterial cell walls. I. Utilization of uridine diphosphate acetylmuramyl pentapeptide and uridine diphosphate acetylglucosamine for peptidoglycan synthesis by particulate enzymes from Staphylococcus aureus and Micrococcus lysodeikticus","volume":"116","author":"Anderson","year":"1966","journal-title":"Arch Biochem Biophys"},{"key":"R3","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1128\/JB.181.2.483-492.1999","article-title":"Use of genomics to identify bacterial undecaprenyl pyrophosphate synthetase: cloning, expression, and characterization of the essential uppS gene","volume":"181","author":"Apfel","year":"1999","journal-title":"J Bacteriol"},{"key":"R4","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1111\/j.1574-6968.1994.tb06898.x","article-title":"From peptidoglycan to glycoproteins: common features of lipid-linked oligosaccharide biosynthesis","volume":"119","author":"Bugg","year":"1994","journal-title":"FEMS Microbiol Lett"},{"key":"R5","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0304-4165(98)00127-5","article-title":"The dolichol pathway of N -linked glycosylation","volume":"1426","author":"Burda","year":"1999","journal-title":"Biochim Biophys Acta"},{"key":"R6","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.1073\/pnas.69.8.2110","article-title":"Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA","volume":"69","author":"Cohen","year":"1972","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R7","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.1073\/pnas.120163297","article-title":"One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products","volume":"97","author":"Datsenko","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R8","doi-asserted-by":"crossref","first-page":"3764","DOI":"10.1128\/JB.177.13.3764-3770.1995","article-title":"Escherichia coli alkaline phosphatase localized to the cytoplasm slowly acquires enzymatic activity in cells whose growth has been suspended: a caution for gene fusion studies","volume":"177","author":"Derman","year":"1995","journal-title":"J Bacteriol"},{"key":"R9","doi-asserted-by":"crossref","first-page":"6127","DOI":"10.1093\/nar\/16.13.6127","article-title":"High efficiency transformation of E. coli by high voltage electroporation","volume":"16","author":"Dower","year":"1988","journal-title":"Nucleic Acids Res"},{"key":"R10","doi-asserted-by":"crossref","first-page":"2690","DOI":"10.1073\/pnas.052018199","article-title":"Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA\/GFP fusion analysis","volume":"99","author":"Drew","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R11","doi-asserted-by":"crossref","first-page":"30106","DOI":"10.1074\/jbc.M401701200","article-title":"The bacA gene of Escherichia coli encodes an undecaprenyl pyrophosphate phosphatase activity","volume":"279","author":"El Ghachi","year":"2004","journal-title":"J Biol Chem"},{"key":"R12","doi-asserted-by":"crossref","first-page":"18689","DOI":"10.1074\/jbc.M412277200","article-title":"Identification of multiple genes encoding membrane proteins with undecaprenyl pyrophosphate phosphatase (UppP) activity in Escherichia coli","volume":"280","author":"El Ghachi","year":"2005","journal-title":"J Biol Chem"},{"key":"R13","doi-asserted-by":"crossref","first-page":"4068","DOI":"10.1128\/JB.182.14.4068-4076.2000","article-title":"Green fluorescent protein functions as a reporter for protein localization in Escherichia coli","volume":"182","author":"Feilmeier","year":"2000","journal-title":"J Bacteriol"},{"key":"R14","doi-asserted-by":"crossref","first-page":"35129","DOI":"10.1074\/jbc.274.49.35129","article-title":"The activity of a putative polyisoprenol-linked sugar translocase (Wzx) involved in Escherichia coli O antigen assembly is independent of the chemical structure of the O repeat","volume":"274","author":"Feldman","year":"1999","journal-title":"J Biol Chem"},{"key":"R15","doi-asserted-by":"crossref","first-page":"41455","DOI":"10.1074\/jbc.M105544200","article-title":"The CWH8 gene encodes a dolichyl pyrophosphate phosphatase with a luminally oriented active site in the endoplasmic reticulum of Saccharomyces cerevisiae","volume":"276","author":"Fernandez","year":"2001","journal-title":"J Biol Chem"},{"key":"R16","doi-asserted-by":"crossref","first-page":"5116","DOI":"10.1016\/S0021-9258(19)44947-8","article-title":"Purification and properties of C55-isoprenylpyrophosphate phosphatase from Micrococcus lysodeikticus","volume":"247","author":"Goldman","year":"1972","journal-title":"J Biol Chem"},{"key":"R17","doi-asserted-by":"crossref","first-page":"4121","DOI":"10.1128\/JB.177.14.4121-4130.1995","article-title":"Tight regulation, modulation, and high-level expression by vectors containing the arabinose pBAD promoter","volume":"177","author":"Guzman","year":"1995","journal-title":"J Bacteriol"},{"key":"R18","doi-asserted-by":"crossref","first-page":"3021","DOI":"10.1002\/j.1460-2075.1986.tb04601.x","article-title":"The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology","volume":"5","author":"Heijne","year":"1986","journal-title":"EMBO J"},{"key":"R19","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S1084-9521(02)00045-9","article-title":"Transmembrane movement of dolichol linked carbohydrates during N -glycoprotein biosynthesis in the endoplasmic reticulum","volume":"13","author":"Helenius","year":"2002","journal-title":"Semin Cell Dev Biol"},{"key":"R20","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1038\/415447a","article-title":"Translocation of lipid-linked oligosaccharides across the ER membrane requires Rft1 protein","volume":"415","author":"Helenius","year":"2002","journal-title":"Nature"},{"key":"R21","first-page":"95","article-title":"Hidden Markov models for sequence analysis: extension and analysis of the basic method","volume":"12","author":"Hughey","year":"1996","journal-title":"Comput Appl Biosci"},{"key":"R22","doi-asserted-by":"crossref","first-page":"2412","DOI":"10.1093\/emboj\/19.11.2412","article-title":"X-ray structures of a novel acid phosphatase from Escherichia blattae and its complex with the transition-state analog molybdate","volume":"19","author":"Ishikawa","year":"2000","journal-title":"EMBO J"},{"key":"R23","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1271\/bbb.66.1619","article-title":"Mevalonate and nonmevalonate pathways for the biosynthesis of isoprene units","volume":"66","author":"Kuzuyama","year":"2002","journal-title":"Biosci Biotechnol Biochem"},{"key":"R24","doi-asserted-by":"crossref","first-page":"7913","DOI":"10.1016\/S0040-4039(98)01755-9","article-title":"Fosmidomycin, a specific inhibitor of 1-deoxy-d-xylulose 5-phosphate reductoisomerase in the nonmevalonate pathway for terpenoid biosynthesis","volume":"39","author":"Kuzuyama","year":"1998a","journal-title":"Tetrahedron Lett"},{"key":"R25","doi-asserted-by":"crossref","first-page":"4509","DOI":"10.1016\/S0040-4039(98)00802-8","article-title":"Direct formation of 2-C-methyl-d-erythritol 4-phosphate from 1-deoxy-d-xylulose 5-phosphate by 1-deoxy-d-xylulose 5-phosphate reductoisomerase, a new enzyme in the non-mevalonate pathway to isopentenyl diphosphate","volume":"39","author":"Kuzuyama","year":"1998b","journal-title":"Tetrahedron Lett"},{"key":"R26","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1099\/13500872-140-1-49","article-title":"Escherichia coli K12 regains its O antigen","volume":"140","author":"Liu","year":"1994","journal-title":"Microbiology"},{"key":"R27","doi-asserted-by":"crossref","first-page":"2105","DOI":"10.1073\/pnas.95.5.2105","article-title":"Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of d-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis","volume":"95","author":"Lois","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R28","doi-asserted-by":"crossref","first-page":"2200","DOI":"10.1128\/AAC.37.10.2200","article-title":"A new method to determine postantibiotic effect and effects of subinhibitory antibiotic concentrations","volume":"37","author":"Lo\u0308wdin","year":"1993","journal-title":"Antimicrob Agents Chemother"},{"key":"R29","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0091-679X(08)61676-3","article-title":"Analysis of membrane protein topology using alkaline phosphatase and beta-galactosidase gene fusions","volume":"34","author":"Manoil","year":"1991","journal-title":"Methods Cell Biol"},{"key":"R30","doi-asserted-by":"crossref","first-page":"3590","DOI":"10.1128\/JB.172.7.3590-3599.1990","article-title":"Genetic analysis of the O7-polysaccharide biosynthesis region from the Escherichia coli O7\u200a:\u200aK1 strain VW187","volume":"172","author":"Marolda","year":"1990","journal-title":"J Bacteriol"},{"key":"R32","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.1002\/pro.5560060817","article-title":"An unexpected structural relationship between integral membrane phosphatases and soluble haloperoxidases","volume":"6","author":"Neuwald","year":"1997","journal-title":"Protein Sci"},{"key":"R33","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1104\/pp.007138","article-title":"Elucidation of the methylerythritol phosphate pathway for isoprenoid biosynthesis in bacteria and plastids. A metabolic milestone achieved through genomics","volume":"130","author":"Rodriguez-Concepcion","year":"2002","journal-title":"Plant Physiol"},{"key":"R34","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1039\/a709175c","article-title":"The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher plants","volume":"16","author":"Rohmer","year":"1999","journal-title":"Nat Prod Rep"},{"key":"R35","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1042\/bj2950517","article-title":"Isoprenoid biosynthesis in bacteria: a novel pathway for the early steps leading to isopentenyl diphosphate","volume":"295","author":"Rohmer","year":"1993","journal-title":"Biochem J"},{"key":"R36","doi-asserted-by":"crossref","first-page":"45226","DOI":"10.1074\/jbc.M207076200","article-title":"Identification and characterization of a cDNA encoding a dolichyl pyrophosphate phosphatase located in the endoplasmic reticulum of mammalian cells","volume":"277","author":"Rush","year":"2002","journal-title":"J Biol Chem"},{"key":"R37","doi-asserted-by":"crossref","first-page":"19476","DOI":"10.1074\/jbc.273.31.19476","article-title":"Molecular cloning, expression, and purification of undecaprenyl diphosphate synthase. No sequence similarity between E- and Z-prenyl diphosphate synthases","volume":"273","author":"Shimizu","year":"1998","journal-title":"J Biol Chem"},{"key":"R38","first-page":"175","article-title":"A hidden Markov model for predicting transmembrane helices in protein sequences","volume-title":"Proceedings of Sixth International Conference on Intelligent Systems for Molecular Biology","author":"Sonnhammer","year":"1998"},{"key":"R39","doi-asserted-by":"crossref","first-page":"12857","DOI":"10.1073\/pnas.94.24.12857","article-title":"Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-d-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol","volume":"94","author":"Sprenger","year":"1997","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R40","first-page":"18401","article-title":"Structural basis of fosmidomycin action revealed by the complex with 2-C-methyl-d-erythritol 4-phosphate synthase (IspC","volume":"278","author":"Steinbacher","year":"2003","journal-title":"Implications for the catalytic mechanism and anti-malaria drug development. J Biol Chem"},{"key":"R41","doi-asserted-by":"crossref","first-page":"4144","DOI":"10.1128\/JB.176.13.4144-4156.1994","article-title":"Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb cluster","volume":"176","author":"Stevenson","year":"1994","journal-title":"J Bacteriol"},{"key":"R42","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1002\/pro.5560060226","article-title":"Identification of a novel phosphatase sequence motif","volume":"6","author":"Stukey","year":"1997","journal-title":"Protein Sci"},{"key":"R43","doi-asserted-by":"crossref","first-page":"9879","DOI":"10.1073\/pnas.95.17.9879","article-title":"A 1-deoxy-d-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-d-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis","volume":"95","author":"Takahashi","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R44","doi-asserted-by":"crossref","first-page":"s452","DOI":"10.2741\/1079","article-title":"Export of O-specific lipopolysaccharide","volume":"8","author":"Valvano","year":"2003","journal-title":"Front Biosci"},{"key":"R45","first-page":"637","article-title":"Biosynthesis of isoprenoids in bacteria","volume-title":"Escherichia coli and Salmonella. Cellular and Molecular Biology","author":"White","year":"1996"},{"key":"R46","doi-asserted-by":"crossref","first-page":"4133","DOI":"10.1128\/JB.176.13.4133-4143.1994","article-title":"Genetic analysis of the O-specific lipopolysaccharide biosynthesis region ( rfb ) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a","volume":"176","author":"Yao","year":"1994","journal-title":"J Bacteriol"},{"key":"R47","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1515\/znc-1998-11-1208","article-title":"Inhibition of the non-mevalonate 1-deoxy-d-xylulose-5-phosphate pathway of plant isoprenoid biosynthesis by fosmidomycin","volume":"[C] 53c","author":"Zeidler","year":"1998","journal-title":"Z Naturforsch"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.2007\/006312-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,20]],"date-time":"2021-08-20T17:39:24Z","timestamp":1629481164000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.2007\/006312-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8,1]]},"references-count":46,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2007,8,1]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.2007\/006312-0","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8,1]]}}}