{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:12:54Z","timestamp":1792174374792,"version":"4.4.0"},"reference-count":59,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,5,1]]},"abstract":"<jats:p>The regulation of the cyanide-insensitive oxidase (CIO) in<jats:italic>Pseudomonas aeruginosa<\/jats:italic>, a bacterium that can synthesize HCN, is reported. The expression of a<jats:italic>cioA\u2013lacZ<\/jats:italic>transcriptional fusion, CioA protein levels and CIO activity were low in exponential phase but induced about fivefold upon entry into stationary phase. Varying the O<jats:sub>2<\/jats:sub>transfer coefficient from 11\u00b75\u2005h<jats:sup>\u22121<\/jats:sup>to 87\u00b74\u2005h<jats:sup>\u22121<\/jats:sup>had no effect on CIO expression and no correlation was observed between CIO induction and the dissolved O<jats:sub>2<\/jats:sub>levels in the growth medium. However, a mutant deleted for the O<jats:sub>2<\/jats:sub>-sensitive transcriptional regulator ANR derepressed CIO expression in an O<jats:sub>2<\/jats:sub>-sensitive manner, with the highest induction occurring under low-O<jats:sub>2<\/jats:sub>conditions. Therefore, CIO expression can respond to a signal generated by low O<jats:sub>2<\/jats:sub>levels, but this response is normally kept in check by ANR repression. ANR may play an important role in preventing overexpression of the CIO in relation to other terminal oxidases. A component present in spent culture medium was able to induce CIO expression. However, experiments with purified<jats:italic>N<\/jats:italic>-butanoyl-<jats:sc>l<\/jats:sc>-homoserine lactone or<jats:italic>N<\/jats:italic>-(3-oxododecanoyl)homoserine lactone ruled out a role for these quorum-sensing molecules in the control of CIO expression. Cyanide was a potent inducer of the CIO at physiologically relevant concentrations and experiments using spent culture medium from a \u0394<jats:italic>hcnB<\/jats:italic>mutant, which is unable to synthesize cyanide, showed that cyanide was the inducing factor present in<jats:italic>P. aeruginosa<\/jats:italic>spent culture medium. However, the finding that in a \u0394<jats:italic>hcnB<\/jats:italic>mutant<jats:italic>cioA\u2013lacZ<\/jats:italic>expression was induced normally upon entry into stationary phase indicated that cyanide was not the endogenous inducer of the terminal oxidase. The authors suggest that the failure of O<jats:sub>2<\/jats:sub>to have an effect on CIO expression in the wild-type can be explained either by the requirement for an additional, stationary-phase-specific inducing signal or by the loss of an exponential-phase-specific repressing signal.<\/jats:p>","DOI":"10.1099\/mic.0.26017-0","type":"journal-article","created":{"date-parts":[[2003,5,1]],"date-time":"2003-05-01T02:03:40Z","timestamp":1051754620000},"page":"1275-1284","source":"Crossref","is-referenced-by-count":66,"title":["Regulation of expression of the cyanide-insensitive terminal oxidase in Pseudomonas aeruginosa"],"prefix":"10.1099","volume":"149","author":[{"given":"Megan","family":"Cooper","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Imperial College London, Sir Alexander Fleming Building, South Kensington Campus, London SW7 2AZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gholam 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