{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T15:05:35Z","timestamp":1791039935375,"version":"4.1.0"},"reference-count":23,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,9,1]]},"abstract":"A novel, fast-growing spirochaete was isolated from the hard tick Hyalomma aegyptium<\/jats:italic> (family Ixodidae<\/jats:italic>, subfamily Metastriata<\/jats:italic>) using Barbour\u2013Stoenner\u2013Kelly (BSK) II medium. Tick samples were taken during the summer of 2000 from the Istanbul area in northwestern Turkey. Sixty-seven of 153 adults (44\u200a%) and 72 of 185 nymphs (39\u200a%) were infected with the novel spirochaete, whereas none of the 20 larvae examined were infected. The optimal growth temperature of the spirochaete in BSK II medium was 34\u201337\u200a\u00b0C, and it could grow at 39\u200a\u00b0C. Doubling times at 34 and 37\u200a\u00b0C were 5\u00b73 and 5\u00b71\u2005h, respectively. Six pure cultures of the spirochaete were obtained and characterized by microscopic observation, sequence analysis of the flagellin gene (flaB<\/jats:italic>), SDS-PAGE and Western blotting. The spirochaete was morphologically similar to those of the genus Borrelia<\/jats:italic> and contained a 41\u2005kDa protein reactive with mAb H9724 specific to the flagellin of a Borrelia<\/jats:italic> species. Polyclonal antibody raised to this spirochaete reacted with several antigen bands, whereas no bands were detected with Borrelia burgdorferi<\/jats:italic>, Borrelia hermsii<\/jats:italic>, Borrelia turicatae<\/jats:italic> and Borrelia parkeri<\/jats:italic>. The flaB<\/jats:italic> sequences of the six isolates showed high similarity, with sequence similarity values ranging from 99\u00b72 to 100\u200a%; however, the similarity of the isolates' flaB<\/jats:italic> sequences to those of the Lyme-disease-related Borrelia<\/jats:italic> and relapsing-fever-associated Borrelia<\/jats:italic> species was less than 90\u200a%. These findings suggest that the unique spirochaete is a member of the genus Borrelia<\/jats:italic>, and differs from previously described Borrelia<\/jats:italic> species.<\/jats:p>","DOI":"10.1099\/mic.0.26464-0","type":"journal-article","created":{"date-parts":[[2003,8,29]],"date-time":"2003-08-29T23:35:15Z","timestamp":1062200115000},"page":"2539-2544","source":"Crossref","is-referenced-by-count":38,"title":["A novel, fast-growing Borrelia sp. isolated from the hard tick Hyalomma aegyptium in Turkey"],"prefix":"10.1099","volume":"149","author":[{"given":"Ece S.","family":"G\u00fcner","sequence":"first","affiliation":[{"name":"Medical School, Yeditepe University, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoya","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Microbiology, and COE Program in the 21st Century, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, 422-8526, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teruki","family":"Kadosaka","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Aichi Medical University, Aichi 480-1195, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyuki","family":"Imai","sequence":"additional","affiliation":[{"name":"Department of Microbiology, and COE Program in the 21st Century, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, 422-8526, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiyuki","family":"Masuzawa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, and COE Program in the 21st Century, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, 422-8526, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","first-page":"10","article-title":"Sur le classement des spirochetes recurrentis","volume":"47","author":"Baltazard","year":"1953","journal-title":"Bull Soc Pathol Exot"},{"key":"R2","first-page":"521","article-title":"Isolation and cultivation of Lyme disease spirochetes","volume":"57","author":"Barbour","year":"1984","journal-title":"Yale J Biol Med"},{"key":"R3","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1128\/MMBR.50.4.381-400.1986","article-title":"Biology of Borrelia species","volume":"50","author":"Barbour","year":"1986","journal-title":"Microbiol Rev"},{"key":"R4","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1128\/IAI.41.2.795-804.1983","article-title":"Lyme disease spirochetes and ixodid tick spirochetes share a common surface antigenic determinant defined by a monoclonal antibody","volume":"41","author":"Barbour","year":"1983","journal-title":"Infect Immun"},{"key":"R5","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1128\/IAI.52.2.549-554.1986","article-title":"A Borrelia -specific monoclonal antibody binds to a flagellar epitope","volume":"52","author":"Barbour","year":"1986","journal-title":"Infect Immun"},{"key":"R6","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1093\/infdis\/173.2.403","article-title":"Identification of an uncultivable Borrelia species in the hard tick Amblyomma americanum : possible agent of a Lyme disease-like illness","volume":"173","author":"Barbour","year":"1996","journal-title":"J Infect Dis"},{"key":"R7","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1099\/00207713-49-4-1793","article-title":"Successful in vitro cultivation of Borrelia duttonii and its comparison with Borrelia recurrentis","volume":"49","author":"Cutler","year":"1999","journal-title":"Int J Syst Bacteriol"},{"key":"R8","doi-asserted-by":"crossref","first-page":"2128","DOI":"10.1128\/JCM.38.6.2128-2133.2000","article-title":"Genetic diversity of Borrelia burgdorferi sensu lato in ticks from mainland Portugal","volume":"38","author":"De Michelis","year":"2000","journal-title":"J Clin Microbiol"},{"key":"R9","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1099\/00207713-45-4-804","article-title":"Genetic and phenotypic analysis of Borrelia miyamotoi sp. nov., isolated from the ixodid tick Ixodes persulcatus , the vector for Lyme disease in Japan","volume":"45","author":"Fukunaga","year":"1995","journal-title":"Int J Syst Bacteriol"},{"key":"R10","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1099\/jmm.0.05205-0","article-title":"First isolation and characterization of Borrelia burgdorferi sensu lato strains from Ixodes ricinus ticks in Turkey","volume":"52","author":"Gu\u0308ner","year":"2003","journal-title":"J Med Microbiol"},{"key":"R11","first-page":"57","article-title":"Genus IV. 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