{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T03:52:57Z","timestamp":1775706777328,"version":"3.50.1"},"reference-count":33,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,11,1]]},"abstract":"<jats:p>An aquaporin gene (<jats:italic>aqpX<\/jats:italic>) was previously detected in the pathogenic bacterium<jats:italic>Brucella abortus<\/jats:italic>. Earlier studies showed that AqpX mediated rapid and large water fluxes in both directions in response to sudden osmotic up- or downshifts. Here, to study the role and the expression of the<jats:italic>aqpX<\/jats:italic>gene in<jats:italic>B. abortus<\/jats:italic>, an<jats:italic>aqpX<\/jats:italic>null mutant was constructed using an<jats:italic>aqpX<\/jats:italic>\u200a:\u200a:\u200a<jats:italic>lacZ<\/jats:italic>gene fusion. This mutant showed no significant difference in growth rate compared to the wild-type strain when grown in rich and minimal media, demonstrating that disruption of the<jats:italic>aqpX<\/jats:italic>gene was not lethal for<jats:italic>B. abortus<\/jats:italic>. The role of the<jats:italic>B. abortus<\/jats:italic>AqpX water channel was investigated by exposing the cells to hypo- and hyperosmolar conditions. While in hyperosmolar environments the growth rate of the knockout mutant was not affected, in hypo-osmolar conditions this mutant showed reduced viability after 50\u2005h of growth.<jats:italic>\u03b2<\/jats:italic>-Galactosidase assays and RT-PCR revealed that<jats:italic>aqpX<\/jats:italic>gene expression and the amount of<jats:italic>aqpX<\/jats:italic>mRNA were markedly increased in hyperosmolar conditions. Moreover,<jats:italic>B. abortus<\/jats:italic><jats:italic>aqpX<\/jats:italic>expression levels were enhanced during the mid-exponential phase of growth. These results indicated that the expression of<jats:italic>aqpX<\/jats:italic>was regulated during the growth curve and induced in hyperosmolar conditions. This report is believed to be the first example of the induction of a bacterial aquaporin in hypertonic conditions.<\/jats:p>","DOI":"10.1099\/mic.0.26678-0","type":"journal-article","created":{"date-parts":[[2003,11,5]],"date-time":"2003-11-05T13:32:46Z","timestamp":1068039166000},"page":"3185-3192","source":"Crossref","is-referenced-by-count":18,"title":["The aquaporin gene aqpX of Brucella abortus is induced in hyperosmotic conditions"],"prefix":"10.1099","volume":"149","author":[{"given":"Rigoberto","family":"Hern\u00e1ndez-Castro","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Coyoacan 04510, Mexico DF, Mexico"},{"name":"Departamento de Biolog\u00eda Molecular, Facultad de Medicina, Universidad de Cantabria, Unidad Asociada al Centro de Investigaciones Biol\u00f3gicas (CSIC), Cardenal Herrera Oria s\/n, 39011 Santander, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Cruz","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular, Facultad de Medicina, Universidad de Cantabria, Unidad Asociada al Centro de Investigaciones Biol\u00f3gicas (CSIC), Cardenal Herrera Oria s\/n, 39011 Santander, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asunci\u00f3n","family":"Seoane","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular, Facultad de Medicina, Universidad de Cantabria, Unidad Asociada al Centro de Investigaciones Biol\u00f3gicas (CSIC), Cardenal Herrera Oria s\/n, 39011 Santander, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Mar\u00eda","family":"Garc\u00eda Lobo","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular, Facultad de Medicina, Universidad de Cantabria, Unidad Asociada al Centro de Investigaciones Biol\u00f3gicas (CSIC), Cardenal Herrera Oria s\/n, 39011 Santander, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1681\/ASN.V114764","article-title":"Homer W. 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