{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T07:03:55Z","timestamp":1791097435146,"version":"4.1.0"},"reference-count":35,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,5,1]]},"abstract":"<jats:p>Previous studies have provided histological evidence of an association between primary<jats:italic>Pneumocystis<\/jats:italic>infection and sudden infant death syndrome (SIDS). The aim of this work was to determine the species of clustered<jats:italic>Pneumocystis<\/jats:italic>organisms found in formalin-fixed paraffin-embedded (FFPE) lung tissue sections from Chilean sudden infant death (SID) victims. This approach needed first to optimize a DNA extraction method from such histological sections. For that purpose, the QIAamp DNA Isolation from Paraffin-Embedded Tissue method (Qiagen) was first tested on FFPE lung tissue sections of immunosuppressed Wistar rats inoculated with rat-derived<jats:italic>Pneumocystis<\/jats:italic>. Successful DNA extraction was assessed by the amplification of a 346\u2005bp fragment of the mitochondrial large subunit rRNA gene of the<jats:italic>Pneumocystis<\/jats:italic>species using a previously described PCR assay. PCR products were analysed by direct sequencing and sequences corresponding to<jats:italic>Pneumocystis carinii<\/jats:italic>were found in all the samples. This method was then applied to FFPE lung tissue sections from Chilean SID victims.<jats:italic>Pneumocystis jirovecii<\/jats:italic>was successfully identified in the three tested samples. In conclusion, an efficient protocol for isolating PCR-ready DNA from FFPE lung tissue sections was developed. It established that the<jats:italic>Pneumocystis<\/jats:italic>species found in the lungs of Chilean SID victims was<jats:italic>P. jirovecii<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/mic.0.26895-0","type":"journal-article","created":{"date-parts":[[2004,5,8]],"date-time":"2004-05-08T00:53:04Z","timestamp":1083977584000},"page":"1167-1172","source":"Crossref","is-referenced-by-count":19,"title":["Molecular typing of Pneumocystis jirovecii found in formalin-fixed paraffin-embedded lung tissue sections from sudden infant death victims"],"prefix":"10.1099","volume":"150","author":[{"given":"M.","family":"Chab\u00e9","sequence":"first","affiliation":[{"name":"Ecology of Parasitism, EA-3609-IFR17, Pasteur Institute of Lille, 1 rue du Professeur-Calmette BP245, 59019 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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