{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T06:46:05Z","timestamp":1784616365111,"version":"3.55.0"},"reference-count":13,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,5,1]]},"abstract":"<jats:p>The genome of<jats:italic>Cryptosporidium parvum<\/jats:italic>contains a relatively small number of introns, which includes the<jats:italic>\u03b2<\/jats:italic>-tubulin gene with only a single intron. Recently, it was observed that the intron was not removed from some of the<jats:italic>\u03b2<\/jats:italic>-tubulin transcripts in the late life cycle stages cultured<jats:italic>in vitro<\/jats:italic>. Although normally spliced<jats:italic>\u03b2<\/jats:italic>-tubulin mRNA was detected in all parasite intracellular stages by RT-PCR (e.g. HCT-8 or Caco-2 cells infected with<jats:italic>C. parvum<\/jats:italic>for 12\u201372\u2005h), at 48\u201372\u2005h post-infection unprocessed<jats:italic>\u03b2<\/jats:italic>-tubulin transcripts containing intact introns started to appear in parasite mRNA within infected host cells. The intron-containing transcripts could be detected by fluorescence<jats:italic>in situ<\/jats:italic>hybridization (FISH) using an intron-specific probe. The intron-containing<jats:italic>\u03b2<\/jats:italic>-tubulin transcripts appeared unique to the<jats:italic>in vitro<\/jats:italic>-cultured<jats:italic>C. parvum<\/jats:italic>, since they were not detected in parasite-infected calves at 72\u2005h. As yet, it is unclear whether the late life cycle stages of<jats:italic>C. parvum<\/jats:italic>are partially deficient in intron-splicing or the intron-splicing processes have merely slowed, both of which would allow the detection of intron-containing transcripts. Another possible explanation is that the decay in transcript processing might simply be due to the onset of parasite death. Nonetheless, the appearance of intron-containing transcripts coincides with the arrest of<jats:italic>C. parvum<\/jats:italic>development<jats:italic>in vitro<\/jats:italic>. This unusual observation prompts speculation that the abnormal intron-splicing of<jats:italic>\u03b2<\/jats:italic>-tubulin transcripts may be one of the factors preventing complete development of this parasite<jats:italic>in vitro<\/jats:italic>. Furthermore, the presence of both processed and unprocessed introns in<jats:italic>\u03b2<\/jats:italic>-tubulin transcripts<jats:italic>in vitro<\/jats:italic>may provide a venue for studying overall mechanisms for intron-splicing in this parasite.<\/jats:p>","DOI":"10.1099\/mic.0.26897-0","type":"journal-article","created":{"date-parts":[[2004,5,8]],"date-time":"2004-05-08T00:53:04Z","timestamp":1083977584000},"page":"1191-1195","source":"Crossref","is-referenced-by-count":13,"title":["Intron-containing \u03b2-tubulin transcripts in Cryptosporidium parvum cultured in vitro"],"prefix":"10.1099","volume":"150","author":[{"given":"Xiaomin","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Pathobiology, Texas A&M University, 4467 TAMU, College Station, TX 77843-4467, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl A.","family":"Lancto","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, University of Minnesota, St Paul, MN 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitchell S.","family":"Abrahamsen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, University of Minnesota, St Paul, MN 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Faculty of Genetics Program, Texas A&M University, 4467 TAMU, College Station, TX 77843-4467, USA"},{"name":"Department of Veterinary Pathobiology, College of Veterinary Pathobiology, Texas A&M University, 4467 TAMU, College Station, TX 77843-4467, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0166-6851(99)00081-X","article-title":"Characterization of 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