{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T07:03:51Z","timestamp":1791097431545,"version":"4.1.0"},"reference-count":70,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,5,1]]},"abstract":"<jats:p><jats:italic>Pneumocystis<\/jats:italic>is a fungal genus that contains multiple species. One member of the genus that has not been formally analysed for its phylogenetic relationships and possible species status is the<jats:italic>Pneumocystis<\/jats:italic>found in laboratory mice,<jats:italic>Pneumocystis murina<\/jats:italic>sp. nov. (type strain ATCC PRA-111<jats:sup>T<\/jats:sup>=CBS 114898<jats:sup>T<\/jats:sup>), formerly known as<jats:italic>Pneumocystis carinii<\/jats:italic>f. sp.<jats:italic>muris<\/jats:italic>. To advance research in this area, approximately 3000\u2005bp of additional DNA sequence were obtained from the locus encoding rRNAs. This sequence and others were used to determine genetic distances between<jats:italic>P. murina<\/jats:italic>and other members of the genus. These distances indicated that<jats:italic>P. murina<\/jats:italic>DNA is most similar to that of the species of<jats:italic>Pneumocystis<\/jats:italic>found in laboratory rats. Nevertheless,<jats:italic>P. murina<\/jats:italic>is at least as diverged from these other<jats:italic>Pneumocystis<\/jats:italic>species as species in other fungal genera are from each other. The 18S rRNA gene sequence divergence exhibited by<jats:italic>P. murina<\/jats:italic>could not be ascribed to accelerated evolution of this gene as similar levels of divergence were observed at seven other loci. When five genes were used to construct phylogenetic trees for five<jats:italic>Pneumocystis<\/jats:italic>taxa, including<jats:italic>P. murina<\/jats:italic>, all the trees had the same topology, indicating that genes do not flow among these taxa. The gene trees were all strongly supported by statistical tests. When sequences from the rRNA-encoding locus were used to estimate the time of divergence of<jats:italic>P. murina<\/jats:italic>, the results indicated that<jats:italic>P. murina<\/jats:italic>is as old as the mouse. Taken together, these data support previous recognition of multiple species in the genus and indicate that<jats:italic>P. murina<\/jats:italic>is a phylogenetic species as well.<\/jats:p>","DOI":"10.1099\/mic.0.26921-0","type":"journal-article","created":{"date-parts":[[2004,5,8]],"date-time":"2004-05-08T00:53:04Z","timestamp":1083977584000},"page":"1153-1165","source":"Crossref","is-referenced-by-count":81,"title":["Phylogenetic identification of Pneumocystis murina sp. nov., a new species in laboratory mice"],"prefix":"10.1099","volume":"150","author":[{"given":"Scott P.","family":"Keely","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267-0524, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jared M.","family":"Fischer","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267-0524, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie T.","family":"Cushion","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Infectious Diseases, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267-0524, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James R.","family":"Stringer","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267-0524, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","first-page":"121S","article-title":"Revised nomenclature for Pneumocystis carinii. 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