{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:53:13Z","timestamp":1787503993218,"version":"build-2736575974"},"reference-count":36,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,5,1]]},"abstract":"\n Enterococcus faecalis divIVA<\/jats:italic>\n (\n divIVA<\/jats:italic>\n Ef<\/jats:sub>\n ) is an essential gene implicated in cell division and chromosome segregation. This gene was disrupted by insertional inactivation creating\n E. faecalis<\/jats:italic>\n JHSR1, which was viable only when a wild-type copy of\n divIVA<\/jats:italic>\n Ef<\/jats:sub>\n was expressed\n in trans<\/jats:italic>\n , confirming the essentiality of the gene. The absence of DivIVA\n Ef<\/jats:sub>\n in\n E. faecalis<\/jats:italic>\n JHSR1 inhibited proper cell division, which resulted in abnormal cell clusters possessing enlarged cells of altered shape instead of the characteristic diplococcal morphology of enterococci. The lower viability of the\n divIVA<\/jats:italic>\n Ef<\/jats:sub>\n mutant is caused by improper nucleoid segregation and impaired septation within the numerous cells generated in each cluster. Overexpression of DivIVA\n Ef<\/jats:sub>\n in\n Escherichia coli<\/jats:italic>\n KJB24 resulted in enlarged cells with disrupted cell division, suggesting that this round\n E. coli<\/jats:italic>\n mutant strain could be used as an indicator for functionality of DivIVA\n Ef<\/jats:sub>\n . A\n Bacillus subtilis divIVA<\/jats:italic>\n mutant was not complemented by DivIVA\n Ef<\/jats:sub>\n , indicating that this protein does not recognize DivIVA-specific target sites in\n B. subtilis<\/jats:italic>\n , or that it does not interact with other proteins of the cell division machinery of this micro-organism. DivIVA\n Ef<\/jats:sub>\n also failed to complement a\n Streptococcus pneumoniae divIVA<\/jats:italic>\n mutant, supporting the phylogenetic distance between\n Enterococcus<\/jats:italic>\n and\n Streptococcus<\/jats:italic>\n . Our results indicate that DivIVA is a species-specific multifunctional protein implicated in cell division and chromosome segregation in\n E. faecalis<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1099\/mic.0.27718-0","type":"journal-article","created":{"date-parts":[[2005,5,3]],"date-time":"2005-05-03T17:28:21Z","timestamp":1115141301000},"page":"1381-1393","source":"Crossref","is-referenced-by-count":56,"title":["Enterococcus faecalis divIVA: an essential gene involved in cell division, cell growth and chromosome segregation"],"prefix":"10.1099","volume":"151","author":[{"given":"Sandra","family":"Ramirez-Arcos","sequence":"first","affiliation":[{"name":"Centre for Research in Biopharmaceuticals and Biotechnology, University of Ottawa, Ottawa, ON, Canada K1H 8M5"},{"name":"Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada K1H 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