{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T21:13:17Z","timestamp":1720818797906},"reference-count":58,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,6,1]]},"abstract":"<jats:p>The expression of bacterial cold-shock proteins (CSPs) is highly induced in response to cold shock, and some CSPs are essential for cells to resume growth at low temperature.<jats:italic>Bordetella bronchiseptica<\/jats:italic>encodes five CSPs (named CspA to CspE) with significant amino acid homology to CspA of<jats:italic>Escherichia coli<\/jats:italic>. In contrast to<jats:italic>E. coli<\/jats:italic>, the insertional knock-out of a single<jats:italic>csp<\/jats:italic>gene (<jats:italic>cspB<\/jats:italic>) strongly affected growth of<jats:italic>B. bronchiseptica<\/jats:italic>independent of temperature. In the case of three of the<jats:italic>csp<\/jats:italic>genes (<jats:italic>cspA<\/jats:italic>,<jats:italic>cspB<\/jats:italic>,<jats:italic>cspC<\/jats:italic>) more than one specific transcript could be detected. The net amount of<jats:italic>cspA<\/jats:italic>,<jats:italic>cspB<\/jats:italic>and<jats:italic>cspC<\/jats:italic>transcripts increased strongly after cold shock, while no such effect could be observed for<jats:italic>cspD<\/jats:italic>and<jats:italic>cspE<\/jats:italic>. The exposure to other stress conditions, including translation inhibitors, heat shock, osmotic stress and nutrient deprivation in the stationary phase, indicated that the<jats:italic>csp<\/jats:italic>genes are also responsive to these conditions. The coding regions of all of the cold-shock genes are preceded by a long non-translated upstream region (5\u2032-UTR). In the case of the<jats:italic>cspB<\/jats:italic>gene, a deletion of parts of this region led to a significant reduction of translation of the resulting truncated transcript, indicating a role of the 5\u2032-UTR in translational control. The cold-shock stimulon was investigated by 2D-PAGE and mass spectrometric characterization, leading to the identification of additional cold-inducible proteins (CIPs). Interestingly, two cold-shock genes (<jats:italic>cspC<\/jats:italic>and<jats:italic>cspD<\/jats:italic>) were found to be under the negative control of the BvgAS system, the main transcriptional regulator of<jats:italic>Bordetella<\/jats:italic>virulence genes. Moreover, a negative effect of slight overexpression of CspB, but not of the other CSPs, on the transcription of the adenylate cyclase toxin CyaA of<jats:italic>Bordetella pertussis<\/jats:italic>was observed, suggesting cross-talk between the CSP-mediated stress response stimulon and the<jats:italic>Bordetella<\/jats:italic>virulence regulon.<\/jats:p>","DOI":"10.1099\/mic.0.27785-0","type":"journal-article","created":{"date-parts":[[2005,6,7]],"date-time":"2005-06-07T19:33:51Z","timestamp":1118172831000},"page":"1895-1909","source":"Crossref","is-referenced-by-count":14,"title":["Identification and regulation of cold-inducible factors of Bordetella bronchiseptica"],"prefix":"10.1099","volume":"151","author":[{"given":"Dorothee","family":"St\u00fcbs","sequence":"first","affiliation":[{"name":"Lehrstuhl f\u00fcr Mikrobiologie, Biozentrum der Universit\u00e4t W\u00fcrzburg, Am Hubland, D-97074 W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thilo M.","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Zentralinstitut f\u00fcr Ern\u00e4hrungs- und Lebensmittelforschung, Abteilung Mikrobiologie, D-85354 Freising, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boris","family":"Schneider","sequence":"additional","affiliation":[{"name":"Lehrstuhl f\u00fcr Mikrobiologie, Biozentrum der Universit\u00e4t W\u00fcrzburg, Am Hubland, D-97074 W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armin","family":"Bosserhoff","sequence":"additional","affiliation":[{"name":"Zentrum f\u00fcr Molekulare Biologie, Universit\u00e4t Heidelberg, D-69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roy","family":"Gross","sequence":"additional","affiliation":[{"name":"Lehrstuhl f\u00fcr Mikrobiologie, Biozentrum der Universit\u00e4t W\u00fcrzburg, Am Hubland, D-97074 W\u00fcrzburg, 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