{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:54:34Z","timestamp":1791255274638,"version":"4.1.0"},"reference-count":47,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,9,1]]},"abstract":"<jats:p><jats:italic>Lactococcus lactis<\/jats:italic>, a lactic acid bacterium widely used for food fermentations, is often exposed to damaging stress conditions. In particular, oxidative stress leads to DNA, protein and membrane damages that can be lethal. As<jats:italic>L. lactis<\/jats:italic>has no catalase, the impact of production of the<jats:italic>Bacillus subtilis<\/jats:italic>haem catalase KatE on its oxidative stress resistance was tested. This cytoplasmic catalase was engineered for extracellular expression in<jats:italic>L. lactis<\/jats:italic>with an optimization strategy based on fusion to the nisin-inducible promoter and a lactococcal signal peptide (SP<jats:sub>Usp45<\/jats:sub>). The production of KatE by<jats:italic>L. lactis<\/jats:italic>conferred an 800-fold increase in survival after 1\u2005h exposure to 4\u2005mM hydrogen peroxide, and a 160-fold greater survival in long-term (3\u2005days) survival of aerated cultures in a<jats:italic>cydA<\/jats:italic>mutant, which is unable to respire. The presence of KatE protected DNA from oxidative damage and limited its degradation after long-term aeration in a<jats:italic>cydA<\/jats:italic>\/<jats:italic>recA<\/jats:italic>mutant, defective in DNA repair.<jats:italic>L. lactis<\/jats:italic>is thus able to produce active catalase that can provide efficient antioxidant activity.<\/jats:p>","DOI":"10.1099\/mic.0.27861-0","type":"journal-article","created":{"date-parts":[[2005,9,8]],"date-time":"2005-09-08T22:34:01Z","timestamp":1126218841000},"page":"3011-3018","source":"Crossref","is-referenced-by-count":60,"title":["High-level resistance to oxidative stress in Lactococcus lactis conferred by Bacillus subtilis catalase KatE"],"prefix":"10.1099","volume":"151","author":[{"given":"T.","family":"Rochat","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Recherches Laiti\u00e8res et de G\u00e9n\u00e9tique Appliqu\u00e9e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Miyoshi","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Federal University of Minas Gerais (UFMG-ICB), Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. J.","family":"Gratadoux","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherches Laiti\u00e8res et de G\u00e9n\u00e9tique Appliqu\u00e9e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Duwat","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherches Laiti\u00e8res et de G\u00e9n\u00e9tique Appliqu\u00e9e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Sourice","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherches Laiti\u00e8res et de G\u00e9n\u00e9tique Appliqu\u00e9e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Federal University of Minas Gerais (UFMG-ICB), Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Langella","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherches Laiti\u00e8res et de G\u00e9n\u00e9tique Appliqu\u00e9e, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1128\/AEM.70.1.603-606.2004","article-title":"Cloning and heterologous expression of hematin-dependent catalase produced by Lactobacillus plantarum CNRZ 1228","volume":"70","author":"Abriouel","year":"2004","journal-title":"Appl Environ Microbiol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"20313","DOI":"10.1074\/jbc.272.33.20313","article-title":"Protein oxidation in aging, disease, and oxidative stress","volume":"272","author":"Berlett","year":"1997","journal-title":"J Biol Chem"},{"key":"R3","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1128\/AEM.68.2.917-922.2002","article-title":"Production of human papillomavirus type 16 E7 protein in Lactococcus lactis","volume":"68","author":"Bermudez-Humaran","year":"2002","journal-title":"Appl Environ Microbiol"},{"key":"R4","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0378-1097(03)00475-0","article-title":"Controlled intra- or extracellular production of staphylococcal nuclease and ovine omega interferon in Lactococcus lactis","volume":"224","author":"Bermudez-Humaran","year":"2003","journal-title":"FEMS Microbiol Lett"},{"key":"R5","doi-asserted-by":"crossref","first-page":"4702","DOI":"10.1128\/AEM.70.8.4702-4710.2004","article-title":"Expression of a heterologous manganese superoxide dismutase gene in intestinal lactobacilli provides protection against hydrogen peroxide toxicity","volume":"70","author":"Bruno-Barcena","year":"2004","journal-title":"Appl Environ Microbiol"},{"key":"R6","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1111\/j.1574-6968.1987.tb02465.x","article-title":"Responses of lactic acid bacteria to oxygen","volume":"46","author":"Condon","year":"1987","journal-title":"FEMS Microbiology Lett"},{"key":"R7","doi-asserted-by":"crossref","first-page":"3434","DOI":"10.1128\/JB.178.12.3434-3439.1996","article-title":"Functional analysis of promoters in the nisin gene cluster of Lactococcus lactis","volume":"178","author":"de Ruyter","year":"1996a","journal-title":"J Bacteriol"},{"key":"R8","doi-asserted-by":"crossref","first-page":"3662","DOI":"10.1128\/AEM.62.10.3662-3667.1996","article-title":"Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin","volume":"62","author":"de Ruyter","year":"1996b","journal-title":"Appl Environ Microbiol"},{"key":"R9","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1111\/j.1365-2958.1995.mmi_17061121.x","article-title":"The recA gene of Lactococcus lactis : characterization and involvement in oxidative and thermal stress","volume":"17","author":"Duwat","year":"1995","journal-title":"Mol Microbiol"},{"key":"R10","doi-asserted-by":"crossref","first-page":"4509","DOI":"10.1128\/JB.183.15.4509-4516.2001","article-title":"Respiration capacity of the fermenting bacterium Lactococcus lactis and its positive effects on growth and survival","volume":"183","author":"Duwat","year":"2001","journal-title":"J Bacteriol"},{"key":"R11","doi-asserted-by":"crossref","first-page":"5598","DOI":"10.1128\/JB.177.19.5598-5605.1995","article-title":"Cloning, nucleotide sequence, and regulation of katE encoding a sigma B-dependent catalase in Bacillus subtilis","volume":"177","author":"Engelmann","year":"1995","journal-title":"J Bacteriol"},{"key":"R12","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1128\/MMBR.55.4.561-585.1991","article-title":"Oxidative stress responses in Escherichia coli and Salmonella typhimurium","volume":"55","author":"Farr","year":"1991","journal-title":"Microbiol Rev"},{"key":"R13","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1242\/jeb.201.8.1203","article-title":"Oxygen toxicity: a radical explanation","volume":"201","author":"Fridovich","year":"1998","journal-title":"J Exp Biol"},{"key":"R14","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1046\/j.1365-2958.2003.03700.x","article-title":"CcpA regulation of aerobic and respiration growth in Lactococcus lactis","volume":"50","author":"Gaudu","year":"2003","journal-title":"Mol Microbiol"},{"key":"R15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/JB.154.1.1-9.1983","article-title":"Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing","volume":"154","author":"Gasson","year":"1983","journal-title":"J Bacteriol"},{"key":"R16","volume-title":"Studies of the Eppstein \u2013 Barr virus genome","author":"Gibson","year":"1984"},{"key":"R17","doi-asserted-by":"crossref","first-page":"29521","DOI":"10.1074\/jbc.271.47.29521","article-title":"Molecular cloning of manganese catalase from Lactobacillus plantarum","volume":"271","author":"Igarashi","year":"1996","journal-title":"J Biol Chem"},{"key":"R18","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1146\/annurev.micro.57.030502.090938","article-title":"Pathways of oxidative damage","volume":"57","author":"Imlay","year":"2003","journal-title":"Annu Rev Microbiol"},{"key":"R19","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1128\/AEM.58.3.832-839.1992","article-title":"Cloning, sequence, and phenotypic expression of katA , which encodes the catalase of Lactobacillus sake LTH677","volume":"58","author":"Knauf","year":"1992","journal-title":"Appl Environ Microbiol"},{"key":"R20","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1046\/j.1365-2036.2002.01378.x","article-title":"Review article: oxidative stress as a pathogenic factor in inflammatory bowel disease \u2013 radicals or ridiculous?","volume":"16","author":"Kruidenier","year":"2002","journal-title":"Aliment Pharmacol Ther"},{"key":"R21","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1002\/path.1409","article-title":"Intestinal oxidative damage in inflammatory bowel disease: semi-quantification, localization, and association with mucosal antioxidants","volume":"201","author":"Kruidenier","year":"2003a","journal-title":"J Pathol"},{"key":"R22","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1002\/path.1407","article-title":"Differential mucosal expression of three superoxide dismutase isoforms in inflammatory bowel disease","volume":"201","author":"Kruidenier","year":"2003b","journal-title":"J Pathol"},{"key":"R23","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1002\/path.1408","article-title":"Imbalanced secondary mucosal antioxidant response in inflammatory bowel disease","volume":"201","author":"Kruidenier","year":"2003c","journal-title":"J Pathol"},{"key":"R24","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1016\/S0891-5849(02)01426-0","article-title":"Attenuated mild colonic inflammation and improved survival from severe DSS-colitis of transgenic Cu\/Zn-SOD mice","volume":"34","author":"Kruidenier","year":"2003d","journal-title":"Free Radic Biol Med"},{"key":"R25","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0168-1656(98)00100-X","article-title":"Quorum sensing-controlled gene expression in lactic acid bacteria","volume":"64","author":"Kuipers","year":"1998","journal-title":"J Biotechnol"},{"key":"R26","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1038\/36786","article-title":"The complete genome sequence of the gram-positive bacterium Bacillus subtilis","volume":"390","author":"Kunst","year":"1997","journal-title":"Nature"},{"key":"R27","doi-asserted-by":"crossref","first-page":"5806","DOI":"10.1128\/JB.175.18.5806-5813.1993","article-title":"Efficient plasmid mobilization by pIP501 in Lactococcus lactis subsp. lactis","volume":"175","author":"Langella","year":"1993","journal-title":"J Bacteriol"},{"key":"R28","doi-asserted-by":"crossref","first-page":"5135","DOI":"10.1128\/JB.176.16.5135-5139.1994","article-title":"Direct screening of recombinants in gram-positive bacteria using the secreted staphylococcal nuclease as a reporter","volume":"176","author":"Le Loir","year":"1994","journal-title":"J Bacteriol"},{"key":"R29","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1128\/JB.180.7.1895-1903.1998","article-title":"A nine-residue synthetic propeptide enhances secretion efficiency of heterologous proteins in Lactococcus lactis","volume":"180","author":"Le Loir","year":"1998","journal-title":"J Bacteriol"},{"key":"R30","doi-asserted-by":"crossref","first-page":"4119","DOI":"10.1128\/AEM.67.9.4119-4127.2001","article-title":"Signal peptide and propeptide optimization for heterologous protein secretion in Lactococcus lactis","volume":"67","author":"Le Loir","year":"2001","journal-title":"Appl Environ Microbiol"},{"key":"R31","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0168-1605(94)90118-X","article-title":"Influence of pH, salt and nitrite on the heme-dependent catalase activity of lactic acid bacteria","volume":"24","author":"Mares","year":"1994","journal-title":"Int J Food Microbiol"},{"key":"R32","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.ijfoodmicro.2004.01.020","article-title":"Expression of the catalase gene katA in starter culture Lactobacillus plantarum TISTR850 tolerates oxidative stress and reduces lipid oxidation in fermented meat product","volume":"95","author":"Noonpakdee","year":"2004","journal-title":"Int J Food Microbiol"},{"key":"R33","first-page":"102","article-title":"Heterologous protein production and delivery systems for Lactococcus lactis","volume":"2","author":"Nouaille","year":"2003","journal-title":"Genet Mol Res"},{"key":"R34","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/0032-9592(94)80033-2","article-title":"Immobilization of catalase on macromolecular supports activated with acid dyes","volume":"28","author":"Pifferi","year":"1993","journal-title":"Process Biochem"},{"key":"R35","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0378-1119(96)00554-9","article-title":"Plasmid vectors for gram-positive bacteria switching from high to low copy number","volume":"183","author":"Renault","year":"1996","journal-title":"Gene"},{"key":"R36","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1111\/j.1365-2958.2004.04217.x","article-title":"Respiration metabolism reduces oxidative and acid stress to improve long-term survival of Lactococcus lactis","volume":"53","author":"Rezai\u0308ki","year":"2004","journal-title":"Mol Microbiol"},{"key":"R37","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1128\/AEM.71.5.2782-2788.2005","article-title":"Lactococcus lactis SpOx spontaneous mutants: a family of oxidative-stress-resistant dairy strains","volume":"71","author":"Rochat","year":"2005","journal-title":"Appl Environ Microbiol"},{"key":"R38","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1007\/BF01645142","article-title":"The involvement of oxygen radicals in microbicidal mechanism of leukocytes and macrophages","volume":"69","author":"Ross","year":"1991","journal-title":"Klin Wochenschr"},{"key":"R39","volume-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"R40","doi-asserted-by":"crossref","first-page":"5254","DOI":"10.1128\/JB.177.18.5254-5260.1995","article-title":"Stress response in Lactococcus lactis : cloning, expression analysis, and mutation of the lactococcal superoxide dismutase gene","volume":"177","author":"Sanders","year":"1995","journal-title":"J Bacteriol"},{"key":"R41","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/0300-9084(88)90093-4","article-title":"Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis","volume":"70","author":"Simon","year":"1988","journal-title":"Biochimie"},{"key":"R42","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1128\/MMBR.57.1.109-137.1993","article-title":"Protein secretion in Bacillus species","volume":"57","author":"Simonen","year":"1993","journal-title":"Microbiol Rev"},{"key":"R43","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/0003-2697(72)90132-7","article-title":"Colorimetric assay of catalase","volume":"47","author":"Sinha","year":"1972","journal-title":"Anal Biochem"},{"key":"R44","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/S1369-5274(99)80033-2","article-title":"Oxidative stress","volume":"2","author":"Storz","year":"1999","journal-title":"Curr Opin Microbiol"},{"key":"R45","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1016\/S0891-5849(97)00291-8","article-title":"Role of antioxidant enzymes on ionizing radiation resistance","volume":"24","author":"Sun","year":"1998","journal-title":"Free Radic Biol Med"},{"key":"R46","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/0378-1119(90)90428-T","article-title":"Cloning of usp45 , a gene encoding a secreted protein from Lactococcus lactis subsp lactis MG1363","volume":"95","author":"van Asseldonk","year":"1990","journal-title":"Gene"},{"key":"R47","doi-asserted-by":"crossref","first-page":"2452","DOI":"10.1128\/AEM.53.10.2452-2457.1987","article-title":"Isolation and characterization of Streptococcus cremoris Wg2-specific promoters","volume":"53","author":"van der Vossen","year":"1987","journal-title":"Appl Environ Microbiol"}],"container-title":["Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.27861-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,17]],"date-time":"2021-07-17T06:28:48Z","timestamp":1626503328000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/micro\/10.1099\/mic.0.27861-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,9,1]]},"references-count":47,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2005,9,1]]}},"URL":"https:\/\/doi.org\/10.1099\/mic.0.27861-0","relation":{},"ISSN":["1350-0872","1465-2080"],"issn-type":[{"value":"1350-0872","type":"print"},{"value":"1465-2080","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,9,1]]}}}