{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:49:34Z","timestamp":1790254174143,"version":"4.1.0"},"reference-count":45,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,6,1]]},"abstract":"<jats:p>Inactivation of the gene encoding DNA adenine methylase (<jats:italic>dam<\/jats:italic>) has been shown to attenuate some pathogens such as<jats:italic>Salmonella enterica<\/jats:italic>serovar Typhimurium and is a lethal mutation in others such as<jats:italic>Yersinia pseudotuberculosis<\/jats:italic>strain YPIII. In this study the<jats:italic>dam<\/jats:italic>methylase gene in<jats:italic>Yersinia pseudotuberculosis<\/jats:italic>strain IP32953 was inactivated. Unlike the wild-type, DNA isolated from the mutant could be digested with<jats:italic>Mbo<\/jats:italic>I, which is consistent with an altered pattern of DNA methylation. The mutant was sensitive to bile salts but not to 2-aminopurine. The effect of<jats:italic>dam<\/jats:italic>inactivation on gene expression was examined using a DNA microarray. In BALB\/c mice inoculated orally or intravenously with the<jats:italic>dam<\/jats:italic>mutant, the median lethal dose (MLD) was at least 10<jats:sup>6<\/jats:sup>-fold higher than the MLD of the wild-type. BALB\/c mice inoculated with the mutant were protected against a subcutaneous challenge with 100 MLDs of<jats:italic>Yersinia pestis<\/jats:italic>strain GB and an intravenous challenge with 300 MLDs of<jats:italic>Y. pseudotuberculosis<\/jats:italic>IP32953.<\/jats:p>","DOI":"10.1099\/mic.0.27959-0","type":"journal-article","created":{"date-parts":[[2005,6,7]],"date-time":"2005-06-07T19:33:51Z","timestamp":1118172831000},"page":"1919-1926","source":"Crossref","is-referenced-by-count":72,"title":["Oral immunization with a dam mutant of Yersinia pseudotuberculosis protects against plague"],"prefix":"10.1099","volume":"151","author":[{"given":"Victoria L.","family":"Taylor","sequence":"first","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard W.","family":"Titball","sequence":"additional","affiliation":[{"name":"Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel St, London WC1E 7HT, UK"},{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra C. 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