{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:38:15Z","timestamp":1791175095397,"version":"4.1.0"},"reference-count":38,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,12,1]]},"abstract":"<jats:p>\n                  <jats:italic>Helicobacter hepaticus<\/jats:italic> is a pathogen of rodents, which causes diverse enteric and hepatic inflammatory diseases and malignancies. The urease enzyme is an important colonization factor of gastric <jats:italic>Helicobacter<\/jats:italic> species like <jats:italic>Helicobacter pylori<\/jats:italic>, but little is known about the role and regulation of urease in enterohepatic <jats:italic>Helicobacter<\/jats:italic> species. Here it is reported that urease activity of <jats:italic>H. hepaticus<\/jats:italic> does not contribute to acid resistance, and that it is nickel-responsive at the post-translational level. <jats:italic>H. hepaticus<\/jats:italic> strain ATCC 51449 did not grow or survive at pH\u20053\u00b70, and supplementation with urea or NiCl<jats:sub>2<\/jats:sub> did not abrogate this acid sensitivity. Furthermore, urease enzyme activity of <jats:italic>H. hepaticus<\/jats:italic> was acid-independent, which contrasts with the acid-induced urease system of <jats:italic>H. pylori<\/jats:italic>. Nickel supplementation of Brucella medium resulted in a tenfold increase in urease activity in both <jats:italic>H. hepaticus<\/jats:italic> and <jats:italic>H. pylori<\/jats:italic>, but the maximum level of urease activity in <jats:italic>H. hepaticus<\/jats:italic> was still three- to fivefold lower when compared to <jats:italic>H. pylori<\/jats:italic> in the same conditions. The increase in urease activity of <jats:italic>H. hepaticus<\/jats:italic> was not associated with elevation of urease mRNA or protein levels. Inhibition of protein synthesis by chloramphenicol did not affect nickel-responsive induction of urease activity in <jats:italic>H. hepaticus<\/jats:italic>, and confirmed that nickel induction occurs at the post-translational level, probably by activation of preformed apo-enzyme. In conclusion, both the role of the urease enzyme and the regulation of urease activity differ between the enterohepatic pathogen <jats:italic>H. hepaticus<\/jats:italic> and the gastric pathogen <jats:italic>H. pylori<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/mic.0.28188-0","type":"journal-article","created":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T21:32:50Z","timestamp":1134163970000},"page":"3989-3995","source":"Crossref","is-referenced-by-count":31,"title":["Differential regulation of urease activity in Helicobacter hepaticus and Helicobacter pylori"],"prefix":"10.1099","volume":"151","author":[{"given":"Clara","family":"Belzer","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Erasmus MC \u2013 University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen","family":"Stoof","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Erasmus MC \u2013 University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine S.","family":"Beckwith","sequence":"additional","affiliation":[{"name":"Department of Comparative Medicine, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernst J.","family":"Kuipers","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Erasmus MC \u2013 University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes G.","family":"Kusters","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Erasmus MC \u2013 University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnoud H. 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