{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T04:12:14Z","timestamp":1777176734970,"version":"3.51.4"},"reference-count":30,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,10,1]]},"abstract":"<jats:p>\n                  <jats:italic>Brucella abortus<\/jats:italic> needs to synthesize haem in order to replicate intracellularly and to produce virulence in mice. Thus, to gain insight into the pathogenesis of the bacterium, regulatory proteins of the haem biosynthetic pathway were sought. An iron response regulator (Irr) from <jats:italic>Bradyrhizobium japonicum<\/jats:italic>, which is a close relative of <jats:italic>Brucella<\/jats:italic>, was previously described as being involved in the coordination of haem biosynthesis and iron availability. The <jats:italic>Bru. abortus<\/jats:italic> genome was searched for an <jats:italic>irr<\/jats:italic> orthologue gene, and the <jats:italic>Bru. abortus irr<\/jats:italic> gene was cloned, sequenced and disrupted. A null mutant was constructed that accumulated protoporphyrin IX under conditions of iron deprivation. This phenotype was overcome by a complementing plasmid carrying the wild-type <jats:italic>irr<\/jats:italic>. Purified recombinant <jats:italic>Bru. abortus<\/jats:italic> Irr behaved as a stable dimer and bound haem. Interestingly, <jats:italic>in vivo<\/jats:italic>, Irr was only detected in cells obtained from iron-limited cultures and the protein downregulated its own transcription. Through <jats:italic>lacZ<\/jats:italic> fusion, it was demonstrated that iron did not regulate <jats:italic>irr<\/jats:italic> transcription. The data reported show that <jats:italic>Bru. abortus<\/jats:italic> Irr is a homodimeric protein that is accumulated in iron-limited cells, controls its own transcription and downregulates the biosynthesis of haem precursors.<\/jats:p>","DOI":"10.1099\/mic.0.28213-0","type":"journal-article","created":{"date-parts":[[2005,10,5]],"date-time":"2005-10-05T22:08:00Z","timestamp":1128550080000},"page":"3427-3433","source":"Crossref","is-referenced-by-count":42,"title":["Dimeric Brucella abortus Irr protein controls its own expression and binds haem"],"prefix":"10.1099","volume":"151","author":[{"given":"Marcela","family":"Mart\u00ednez","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas, Instituto Tecnol\u00f3gico de Chascom\u00fas (IIB, INTECH), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Universidad Nacional de General San Mart\u00edn (CONICET-UNSAM), Ed 24, Av. 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